<i>Bacillus amyloliquefaciens</i> Probiotics Mix Supplementation in a Broiler Leaky Gut Model
The supplementation of antimicrobial growth promoters (AGPs) has been banned in many countries because of the emergence of antimicrobial-resistant pathogens in poultry products and the environment. Probiotics have been broadly studied and demonstrated as a promising AGP substitute. Our study is cent...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/2/419 |
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| author | Darwin Horyanto Yadav S. Bajagai Advait Kayal Juhani von Hellens Xiaojing Chen Thi Thu Hao Van Anita Radovanović Dragana Stanley |
| author_facet | Darwin Horyanto Yadav S. Bajagai Advait Kayal Juhani von Hellens Xiaojing Chen Thi Thu Hao Van Anita Radovanović Dragana Stanley |
| author_sort | Darwin Horyanto |
| collection | DOAJ |
| description | The supplementation of antimicrobial growth promoters (AGPs) has been banned in many countries because of the emergence of antimicrobial-resistant pathogens in poultry products and the environment. Probiotics have been broadly studied and demonstrated as a promising AGP substitute. Our study is centred on the effects of a multi-strain <i>Bacillus</i>-based probiotic product on broiler production performance and gut microbial profile in a dexamethasone-induced leaky gut challenge. Two hundred and fifty-six broiler chicks were hatched and randomly assigned into four groups (wheat-soybean meal basal diet (BD) = non-supplemented control (C), BD supplemented with dexamethasone in week 4 (CD), BD containing a probiotic from day one (P), and BD containing a probiotic from day one and supplemented with dexamethasone during challenge week 4 (PD)). The production performance and caecal, gizzard, jejunal lumen and jejunal mucosa swab microbiota were studied by 16S rRNA gene sequencing. The <i>Bacillus</i> probiotic product significantly improved production performance and altered caecal gut microbiota (<i>p</i> ≤ 0.05), but no significant impact on microbiota was observed in other gut sections. |
| first_indexed | 2024-03-07T22:20:11Z |
| format | Article |
| id | doaj.art-c0f97da52125410083973966c3231a68 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-07T22:20:11Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c0f97da52125410083973966c3231a682024-02-23T15:28:29ZengMDPI AGMicroorganisms2076-26072024-02-0112241910.3390/microorganisms12020419<i>Bacillus amyloliquefaciens</i> Probiotics Mix Supplementation in a Broiler Leaky Gut ModelDarwin Horyanto0Yadav S. Bajagai1Advait Kayal2Juhani von Hellens3Xiaojing Chen4Thi Thu Hao Van5Anita Radovanović6Dragana Stanley7Institute for Future Farming Systems, Central Queensland University, Rockhampton, QLD 4702, AustraliaInstitute for Future Farming Systems, Central Queensland University, Rockhampton, QLD 4702, AustraliaInstitute for Future Farming Systems, Central Queensland University, Rockhampton, QLD 4702, AustraliaBioproton Pty Ltd., Acacia Ridge, QLD 4110, AustraliaBioproton Pty Ltd., Acacia Ridge, QLD 4110, AustraliaSchool of Science, RMIT University, Bundoora, VIC 3083, AustraliaFaculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, SerbiaInstitute for Future Farming Systems, Central Queensland University, Rockhampton, QLD 4702, AustraliaThe supplementation of antimicrobial growth promoters (AGPs) has been banned in many countries because of the emergence of antimicrobial-resistant pathogens in poultry products and the environment. Probiotics have been broadly studied and demonstrated as a promising AGP substitute. Our study is centred on the effects of a multi-strain <i>Bacillus</i>-based probiotic product on broiler production performance and gut microbial profile in a dexamethasone-induced leaky gut challenge. Two hundred and fifty-six broiler chicks were hatched and randomly assigned into four groups (wheat-soybean meal basal diet (BD) = non-supplemented control (C), BD supplemented with dexamethasone in week 4 (CD), BD containing a probiotic from day one (P), and BD containing a probiotic from day one and supplemented with dexamethasone during challenge week 4 (PD)). The production performance and caecal, gizzard, jejunal lumen and jejunal mucosa swab microbiota were studied by 16S rRNA gene sequencing. The <i>Bacillus</i> probiotic product significantly improved production performance and altered caecal gut microbiota (<i>p</i> ≤ 0.05), but no significant impact on microbiota was observed in other gut sections.https://www.mdpi.com/2076-2607/12/2/419<i>Bacillus</i>probioticsgut microbiotacolonisationbroilersdexamethasone |
| spellingShingle | Darwin Horyanto Yadav S. Bajagai Advait Kayal Juhani von Hellens Xiaojing Chen Thi Thu Hao Van Anita Radovanović Dragana Stanley <i>Bacillus amyloliquefaciens</i> Probiotics Mix Supplementation in a Broiler Leaky Gut Model Microorganisms <i>Bacillus</i> probiotics gut microbiota colonisation broilers dexamethasone |
| title | <i>Bacillus amyloliquefaciens</i> Probiotics Mix Supplementation in a Broiler Leaky Gut Model |
| title_full | <i>Bacillus amyloliquefaciens</i> Probiotics Mix Supplementation in a Broiler Leaky Gut Model |
| title_fullStr | <i>Bacillus amyloliquefaciens</i> Probiotics Mix Supplementation in a Broiler Leaky Gut Model |
| title_full_unstemmed | <i>Bacillus amyloliquefaciens</i> Probiotics Mix Supplementation in a Broiler Leaky Gut Model |
| title_short | <i>Bacillus amyloliquefaciens</i> Probiotics Mix Supplementation in a Broiler Leaky Gut Model |
| title_sort | i bacillus amyloliquefaciens i probiotics mix supplementation in a broiler leaky gut model |
| topic | <i>Bacillus</i> probiotics gut microbiota colonisation broilers dexamethasone |
| url | https://www.mdpi.com/2076-2607/12/2/419 |
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