Composition and niche-specific characteristics of microbial consortia colonizing Marsberg copper mine in the Rhenish Massif
<p>The Kilianstollen Marsberg (Rhenish Massif, Germany) has been extensively mined for copper ores, dating from early medieval period until 1945. The exposed organic-rich alum shale rocks influenced by the diverse mine drainages at an ambient temperature of 10 <span class="inline-formu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Copernicus Publications
2022-10-01
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| Series: | Biogeosciences |
| Online Access: | https://bg.copernicus.org/articles/19/4883/2022/bg-19-4883-2022.pdf |
| _version_ | 1811340814001897472 |
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| author | S. Arif H. Nacke E. Schliekmann A. Reimer G. Arp M. Hoppert |
| author_facet | S. Arif H. Nacke E. Schliekmann A. Reimer G. Arp M. Hoppert |
| author_sort | S. Arif |
| collection | DOAJ |
| description | <p>The Kilianstollen Marsberg (Rhenish Massif, Germany) has
been extensively mined for copper ores, dating from early medieval period
until 1945. The exposed organic-rich alum shale rocks influenced by the
diverse mine drainages at an ambient temperature of 10 <span class="inline-formula"><sup>∘</sup></span>C could
naturally enrich biogeochemically distinct heavy metal resistant microbiota.
This amplicon-sequence-based study evaluates the microbially colonized
subterranean rocks of the abandoned copper mine Kilianstollen to
characterize the colonization patterns and biogeochemical pathways of
individual microbial groups. Under the selective pressure of the heavy metal
contaminated environment at illuminated sites, Chloroflexi (Ktedonobacteria) and Cyanobacteria (Oxyphotobacteria) build up
whitish–greenish biofilms. In contrast, Proteobacteria, Firmicutes and Actinobacteria dominate rocks around the
uncontaminated spring water streams. The additional metagenomic analysis
revealed that the heavy metal resistant microbiome was evidently involved in
redox cycling of transition metals (Cu, Zn, Co, Ni, Mn, Fe, Cd, Hg). No
deposition of metals or minerals, though, was observed by transmission
electron microscopy in Ktedonobacteria biofilms which may be indicative for the presence of
different detoxification pathways. The underlying heavy metal resistance
mechanisms, as revealed by analysis of metagenome-assembled genomes, were
mainly attributed to transition metal efflux pumps, redox enzymes,
volatilization of Hg, methylated intermediates of As<span class="inline-formula"><sup>3+</sup></span>, and reactive
oxygen species detoxification pathways.</p> |
| first_indexed | 2024-04-13T18:47:32Z |
| format | Article |
| id | doaj.art-c0fa3fa4157d4077ae1b862fb7eaa858 |
| institution | Directory Open Access Journal |
| issn | 1726-4170 1726-4189 |
| language | English |
| last_indexed | 2024-04-13T18:47:32Z |
| publishDate | 2022-10-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Biogeosciences |
| spelling | doaj.art-c0fa3fa4157d4077ae1b862fb7eaa8582022-12-22T02:34:33ZengCopernicus PublicationsBiogeosciences1726-41701726-41892022-10-01194883490210.5194/bg-19-4883-2022Composition and niche-specific characteristics of microbial consortia colonizing Marsberg copper mine in the Rhenish MassifS. Arif0H. Nacke1E. Schliekmann2A. Reimer3G. Arp4M. Hoppert5Department of General Microbiology, Institute of Microbiology and Genetics, Georg-August-Universität, 37077 Göttingen, GermanyDepartment of Genomic and Applied Microbiology, Institute of Microbiology and Genetics, Georg-August-Universität, 37077 Göttingen, GermanyDepartment of General Microbiology, Institute of Microbiology and Genetics, Georg-August-Universität, 37077 Göttingen, GermanyGeoscience Centre, Department of Geobiology, Georg-August-Universität Göttingen, 37077 Göttingen, GermanyGeoscience Centre, Department of Geobiology, Georg-August-Universität Göttingen, 37077 Göttingen, GermanyDepartment of General Microbiology, Institute of Microbiology and Genetics, Georg-August-Universität, 37077 Göttingen, Germany<p>The Kilianstollen Marsberg (Rhenish Massif, Germany) has been extensively mined for copper ores, dating from early medieval period until 1945. The exposed organic-rich alum shale rocks influenced by the diverse mine drainages at an ambient temperature of 10 <span class="inline-formula"><sup>∘</sup></span>C could naturally enrich biogeochemically distinct heavy metal resistant microbiota. This amplicon-sequence-based study evaluates the microbially colonized subterranean rocks of the abandoned copper mine Kilianstollen to characterize the colonization patterns and biogeochemical pathways of individual microbial groups. Under the selective pressure of the heavy metal contaminated environment at illuminated sites, Chloroflexi (Ktedonobacteria) and Cyanobacteria (Oxyphotobacteria) build up whitish–greenish biofilms. In contrast, Proteobacteria, Firmicutes and Actinobacteria dominate rocks around the uncontaminated spring water streams. The additional metagenomic analysis revealed that the heavy metal resistant microbiome was evidently involved in redox cycling of transition metals (Cu, Zn, Co, Ni, Mn, Fe, Cd, Hg). No deposition of metals or minerals, though, was observed by transmission electron microscopy in Ktedonobacteria biofilms which may be indicative for the presence of different detoxification pathways. The underlying heavy metal resistance mechanisms, as revealed by analysis of metagenome-assembled genomes, were mainly attributed to transition metal efflux pumps, redox enzymes, volatilization of Hg, methylated intermediates of As<span class="inline-formula"><sup>3+</sup></span>, and reactive oxygen species detoxification pathways.</p>https://bg.copernicus.org/articles/19/4883/2022/bg-19-4883-2022.pdf |
| spellingShingle | S. Arif H. Nacke E. Schliekmann A. Reimer G. Arp M. Hoppert Composition and niche-specific characteristics of microbial consortia colonizing Marsberg copper mine in the Rhenish Massif Biogeosciences |
| title | Composition and niche-specific characteristics of microbial consortia colonizing Marsberg copper mine in the Rhenish Massif |
| title_full | Composition and niche-specific characteristics of microbial consortia colonizing Marsberg copper mine in the Rhenish Massif |
| title_fullStr | Composition and niche-specific characteristics of microbial consortia colonizing Marsberg copper mine in the Rhenish Massif |
| title_full_unstemmed | Composition and niche-specific characteristics of microbial consortia colonizing Marsberg copper mine in the Rhenish Massif |
| title_short | Composition and niche-specific characteristics of microbial consortia colonizing Marsberg copper mine in the Rhenish Massif |
| title_sort | composition and niche specific characteristics of microbial consortia colonizing marsberg copper mine in the rhenish massif |
| url | https://bg.copernicus.org/articles/19/4883/2022/bg-19-4883-2022.pdf |
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