Huangshui Polysaccharide Exerts Intestinal Barrier Protective Effects through the TLR4/MyD88/NF-<i>κ</i>B and MAPK Signaling Pathways in Caco-2 Cells
Several reports have demonstrated that natural polysaccharides exert protective effects on intestinal barrier function. In our previous study, we isolated a polysaccharide named HSP-W from Huangshui (HS). In the present study, the protective role of HSP-W in LPS-induced intestinal barrier dysfunctio...
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MDPI AG
2023-01-01
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| author | Jiaying Huo Wenhao Pei Guoying Liu Weizheng Sun Jihong Wu Mingquan Huang Wei Lu Jinyuan Sun Baoguo Sun |
| author_facet | Jiaying Huo Wenhao Pei Guoying Liu Weizheng Sun Jihong Wu Mingquan Huang Wei Lu Jinyuan Sun Baoguo Sun |
| author_sort | Jiaying Huo |
| collection | DOAJ |
| description | Several reports have demonstrated that natural polysaccharides exert protective effects on intestinal barrier function. In our previous study, we isolated a polysaccharide named HSP-W from Huangshui (HS). In the present study, the protective role of HSP-W in LPS-induced intestinal barrier dysfunction was determined by several molecular biological techniques. The results showed that HSP-W treatment alleviated the deduced TEER and increased the permeability of intestinal epithelial cells induced by LPS through inhibiting the release of inflammatory cytokines and enhancing the expression of tight junction (TJ) proteins. The underlying molecular mechanisms were elucidated by RNA-seq technique, which indicated that the differentially expressed genes (DEGs) between the LPS-treated and LPS+HSP-W-treated groups were enriched in the “MAPK” signaling pathway. Notably, the overlapping DEGs reversed by HSP-W intervention highlighted the pathways of the “Toll-like receptor” and “NF-<i>κ</i>B” signaling pathways. The suppression of p38 and NF-<i>κ</i>B were mediated by the inhibition of MyD88. Furthermore, HSP-W treatment prevented the translocation of NF-<i>κ</i>B to nucleus, thus inhibiting the release of TNF-<i>α</i>, IL-6, and IL-1<i>β</i>. Overall, HSP-W has beneficial effects on LPS-induced inflammation; it protects the intestinal barrier from injury in Caco-2 cells through inhibiting the TLR4/MyD88/NF-<i>κ</i>B and p38 MAPK signaling pathways. |
| first_indexed | 2024-03-11T09:45:22Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T09:45:22Z |
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| series | Foods |
| spelling | doaj.art-c0fc31eeebe44e2e9f8f2cd5460354d82023-11-16T16:39:30ZengMDPI AGFoods2304-81582023-01-0112345010.3390/foods12030450Huangshui Polysaccharide Exerts Intestinal Barrier Protective Effects through the TLR4/MyD88/NF-<i>κ</i>B and MAPK Signaling Pathways in Caco-2 CellsJiaying Huo0Wenhao Pei1Guoying Liu2Weizheng Sun3Jihong Wu4Mingquan Huang5Wei Lu6Jinyuan Sun7Baoguo Sun8Key Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, ChinaKey Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, ChinaAnhui Gujing Distillery Co. Ltd., Bozhou 236820, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaKey Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, ChinaKey Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, ChinaAnhui Gujing Distillery Co. Ltd., Bozhou 236820, ChinaKey Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, ChinaKey Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, ChinaSeveral reports have demonstrated that natural polysaccharides exert protective effects on intestinal barrier function. In our previous study, we isolated a polysaccharide named HSP-W from Huangshui (HS). In the present study, the protective role of HSP-W in LPS-induced intestinal barrier dysfunction was determined by several molecular biological techniques. The results showed that HSP-W treatment alleviated the deduced TEER and increased the permeability of intestinal epithelial cells induced by LPS through inhibiting the release of inflammatory cytokines and enhancing the expression of tight junction (TJ) proteins. The underlying molecular mechanisms were elucidated by RNA-seq technique, which indicated that the differentially expressed genes (DEGs) between the LPS-treated and LPS+HSP-W-treated groups were enriched in the “MAPK” signaling pathway. Notably, the overlapping DEGs reversed by HSP-W intervention highlighted the pathways of the “Toll-like receptor” and “NF-<i>κ</i>B” signaling pathways. The suppression of p38 and NF-<i>κ</i>B were mediated by the inhibition of MyD88. Furthermore, HSP-W treatment prevented the translocation of NF-<i>κ</i>B to nucleus, thus inhibiting the release of TNF-<i>α</i>, IL-6, and IL-1<i>β</i>. Overall, HSP-W has beneficial effects on LPS-induced inflammation; it protects the intestinal barrier from injury in Caco-2 cells through inhibiting the TLR4/MyD88/NF-<i>κ</i>B and p38 MAPK signaling pathways.https://www.mdpi.com/2304-8158/12/3/450Huangshui<i>α</i>-D-glucanintestinal barrier protectionTLR4/MyD88/NF-<i>κ</i>Bp38 MAPKRNA-seq |
| spellingShingle | Jiaying Huo Wenhao Pei Guoying Liu Weizheng Sun Jihong Wu Mingquan Huang Wei Lu Jinyuan Sun Baoguo Sun Huangshui Polysaccharide Exerts Intestinal Barrier Protective Effects through the TLR4/MyD88/NF-<i>κ</i>B and MAPK Signaling Pathways in Caco-2 Cells Foods Huangshui <i>α</i>-D-glucan intestinal barrier protection TLR4/MyD88/NF-<i>κ</i>B p38 MAPK RNA-seq |
| title | Huangshui Polysaccharide Exerts Intestinal Barrier Protective Effects through the TLR4/MyD88/NF-<i>κ</i>B and MAPK Signaling Pathways in Caco-2 Cells |
| title_full | Huangshui Polysaccharide Exerts Intestinal Barrier Protective Effects through the TLR4/MyD88/NF-<i>κ</i>B and MAPK Signaling Pathways in Caco-2 Cells |
| title_fullStr | Huangshui Polysaccharide Exerts Intestinal Barrier Protective Effects through the TLR4/MyD88/NF-<i>κ</i>B and MAPK Signaling Pathways in Caco-2 Cells |
| title_full_unstemmed | Huangshui Polysaccharide Exerts Intestinal Barrier Protective Effects through the TLR4/MyD88/NF-<i>κ</i>B and MAPK Signaling Pathways in Caco-2 Cells |
| title_short | Huangshui Polysaccharide Exerts Intestinal Barrier Protective Effects through the TLR4/MyD88/NF-<i>κ</i>B and MAPK Signaling Pathways in Caco-2 Cells |
| title_sort | huangshui polysaccharide exerts intestinal barrier protective effects through the tlr4 myd88 nf i κ i b and mapk signaling pathways in caco 2 cells |
| topic | Huangshui <i>α</i>-D-glucan intestinal barrier protection TLR4/MyD88/NF-<i>κ</i>B p38 MAPK RNA-seq |
| url | https://www.mdpi.com/2304-8158/12/3/450 |
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