HPLC Analysis and In Vivo Renoprotective Evaluation of Hydroalcoholic Extract of <i>Cucumis melo</i> Seeds in Gentamicin-Induced Renal Damage
<i>Background and objectives: Cucumis melo</i>, of family Cucurbitaceae, has traditionally been used to treat variety of kidney disorders. However to best of our knowledge there is no scientific study available that validates its renaoprotective uses. Therefore, this study aimed to evalu...
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MDPI AG
2019-04-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1010-660X/55/4/107 |
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| author | Mohammad Saleem Fatima Javed Muhammad Asif Muhammad Kashif Baig Mehwish Arif |
| author_facet | Mohammad Saleem Fatima Javed Muhammad Asif Muhammad Kashif Baig Mehwish Arif |
| author_sort | Mohammad Saleem |
| collection | DOAJ |
| description | <i>Background and objectives: Cucumis melo</i>, of family Cucurbitaceae, has traditionally been used to treat variety of kidney disorders. However to best of our knowledge there is no scientific study available that validates its renaoprotective uses. Therefore, this study aimed to evaluate nephroprotective effects of hydroalcoholic extract of <i>Cucumis melo</i> seeds (CMHE) and to identify its phytoconstituents. <i>Materials and Methods</i>: HPLC was performed to identify key phytochemicals of CMHE. Gentamicin (100 mg/kg/day, i.p) was administered to induce nephrotoxicity in Swiss albino mice for 8 days. Gentamicin (100 mg/kg/day, i.p) and oral CMHE were co-administered to mice at doses of 250 and 500 mg/kg to evaluate protective effects of CMHE. Normal control group mice were administered normal saline. Changes in body weights, biochemical and histopathological studies were conducted to establish nephroprotective effects of CMHE. <i>Results</i>: HPLC analysis indicated presence of quercetin, <i>m</i>-coumaric acid, gallic acid, chlorogenic acid, and <i>trans</i>-4-hydroxy-3-methoxy cinnamic acid in CMHE. Mice treated with CMHE showed significant increase in body weight and decrease in kidney weight as compared with toxic control group. Dose-dependent significant decrease in total blood urea nitrogen, serum creatinine, serum urea, and uric acid levels were observed in CMHE-treated groups as compared with toxic control group. Histopathological analysis of CMHE-treated groups showed improvement in kidney structures as compared with toxic control group. <i>Conclusions</i>: Biochemical, histopathological, and phytochemical screening of hydroalcoholic extract of <i>Cucumis melo</i> seeds suggest that it has nephroprotective potential. Furthermore, standardization of extract against identified phytochemicals, as well as long-term toxicological studies are suggested before commencement of clinical trials. |
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| language | English |
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| publishDate | 2019-04-01 |
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| series | Medicina |
| spelling | doaj.art-c0ff1358cf9040c7bb9b4d58a45751a02023-09-02T14:13:38ZengMDPI AGMedicina1010-660X2019-04-0155410710.3390/medicina55040107medicina55040107HPLC Analysis and In Vivo Renoprotective Evaluation of Hydroalcoholic Extract of <i>Cucumis melo</i> Seeds in Gentamicin-Induced Renal DamageMohammad Saleem0Fatima Javed1Muhammad Asif2Muhammad Kashif Baig3Mehwish Arif4Department of Pharmacology, University College of Pharmacy, University of the Punjab, Lahore, Punjab 54000, PakistanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Punjab 38000, PakistanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Punjab 38000, PakistanDepartment of Pathology, Aziz Fatima Medical and Dental College, Faisalabad, Punjab 38000, PakistanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Punjab 38000, Pakistan<i>Background and objectives: Cucumis melo</i>, of family Cucurbitaceae, has traditionally been used to treat variety of kidney disorders. However to best of our knowledge there is no scientific study available that validates its renaoprotective uses. Therefore, this study aimed to evaluate nephroprotective effects of hydroalcoholic extract of <i>Cucumis melo</i> seeds (CMHE) and to identify its phytoconstituents. <i>Materials and Methods</i>: HPLC was performed to identify key phytochemicals of CMHE. Gentamicin (100 mg/kg/day, i.p) was administered to induce nephrotoxicity in Swiss albino mice for 8 days. Gentamicin (100 mg/kg/day, i.p) and oral CMHE were co-administered to mice at doses of 250 and 500 mg/kg to evaluate protective effects of CMHE. Normal control group mice were administered normal saline. Changes in body weights, biochemical and histopathological studies were conducted to establish nephroprotective effects of CMHE. <i>Results</i>: HPLC analysis indicated presence of quercetin, <i>m</i>-coumaric acid, gallic acid, chlorogenic acid, and <i>trans</i>-4-hydroxy-3-methoxy cinnamic acid in CMHE. Mice treated with CMHE showed significant increase in body weight and decrease in kidney weight as compared with toxic control group. Dose-dependent significant decrease in total blood urea nitrogen, serum creatinine, serum urea, and uric acid levels were observed in CMHE-treated groups as compared with toxic control group. Histopathological analysis of CMHE-treated groups showed improvement in kidney structures as compared with toxic control group. <i>Conclusions</i>: Biochemical, histopathological, and phytochemical screening of hydroalcoholic extract of <i>Cucumis melo</i> seeds suggest that it has nephroprotective potential. Furthermore, standardization of extract against identified phytochemicals, as well as long-term toxicological studies are suggested before commencement of clinical trials.https://www.mdpi.com/1010-660X/55/4/107nephroprotective effect<i>Cucumis melo</i> seedshydroalcoholic extractgentamicinHPLC analysis |
| spellingShingle | Mohammad Saleem Fatima Javed Muhammad Asif Muhammad Kashif Baig Mehwish Arif HPLC Analysis and In Vivo Renoprotective Evaluation of Hydroalcoholic Extract of <i>Cucumis melo</i> Seeds in Gentamicin-Induced Renal Damage Medicina nephroprotective effect <i>Cucumis melo</i> seeds hydroalcoholic extract gentamicin HPLC analysis |
| title | HPLC Analysis and In Vivo Renoprotective Evaluation of Hydroalcoholic Extract of <i>Cucumis melo</i> Seeds in Gentamicin-Induced Renal Damage |
| title_full | HPLC Analysis and In Vivo Renoprotective Evaluation of Hydroalcoholic Extract of <i>Cucumis melo</i> Seeds in Gentamicin-Induced Renal Damage |
| title_fullStr | HPLC Analysis and In Vivo Renoprotective Evaluation of Hydroalcoholic Extract of <i>Cucumis melo</i> Seeds in Gentamicin-Induced Renal Damage |
| title_full_unstemmed | HPLC Analysis and In Vivo Renoprotective Evaluation of Hydroalcoholic Extract of <i>Cucumis melo</i> Seeds in Gentamicin-Induced Renal Damage |
| title_short | HPLC Analysis and In Vivo Renoprotective Evaluation of Hydroalcoholic Extract of <i>Cucumis melo</i> Seeds in Gentamicin-Induced Renal Damage |
| title_sort | hplc analysis and in vivo renoprotective evaluation of hydroalcoholic extract of i cucumis melo i seeds in gentamicin induced renal damage |
| topic | nephroprotective effect <i>Cucumis melo</i> seeds hydroalcoholic extract gentamicin HPLC analysis |
| url | https://www.mdpi.com/1010-660X/55/4/107 |
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