Molecular biomarkers for oxidative stress and neuronal damage in red-bellied pacu (Piaractus brachypomus)
Biomarkers are biological molecules that provide information about the physiological condition of an organism such as early signals of health deterioration. The aim of this study was to compare the relative gene expression of four molecular biomarkers related to oxidative stress, and neuronal damage...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-11-01
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| Series: | Aquaculture and Fisheries |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468550X22001435 |
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| author | Julián David Ortiz Muñoz Kelly Johanna Lozano Villegas Iang Schroniltgen Rondón Barragán |
| author_facet | Julián David Ortiz Muñoz Kelly Johanna Lozano Villegas Iang Schroniltgen Rondón Barragán |
| author_sort | Julián David Ortiz Muñoz |
| collection | DOAJ |
| description | Biomarkers are biological molecules that provide information about the physiological condition of an organism such as early signals of health deterioration. The aim of this study was to compare the relative gene expression of four molecular biomarkers related to oxidative stress, and neuronal damage in Piaractus brachypomus under three different conditions (exposure to chlorpyrifos (CPF), menthol and eugenol anesthesia, and brain injury). Bioinformatic analyses were conducted to design primers for the molecular biomarkers, then, the relative gene expression of COX1, GPX1, BDNF and CALB1 genes was measured by qPCR using brain tissue cDNA from the treated animals as template and 2-ΔΔCT method was performed. EF1a gene was used as reference gene. The results showed a response to chemical stress in the CPF treatment samples where GPX1, BDNF and CALB1 genes were up-regulated. Finally, in menthol/eugenol and brain injury treatments, no alterations in the relative gene expression were observed. |
| first_indexed | 2024-04-09T17:20:54Z |
| format | Article |
| id | doaj.art-c1002edd780f4a93aea1b3a6f1099ce8 |
| institution | Directory Open Access Journal |
| issn | 2468-550X |
| language | English |
| last_indexed | 2024-04-09T17:20:54Z |
| publishDate | 2023-11-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Aquaculture and Fisheries |
| spelling | doaj.art-c1002edd780f4a93aea1b3a6f1099ce82023-04-19T04:23:08ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2023-11-0186720726Molecular biomarkers for oxidative stress and neuronal damage in red-bellied pacu (Piaractus brachypomus)Julián David Ortiz Muñoz0Kelly Johanna Lozano Villegas1Iang Schroniltgen Rondón Barragán2Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad Del Tolima, Santa Helena Highs, Ibagué-Tolima, 730006299, ColombiaResearch Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad Del Tolima, Santa Helena Highs, Ibagué-Tolima, 730006299, ColombiaResearch Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad Del Tolima, Santa Helena Highs, Ibagué-Tolima, 730006299, Colombia; Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad Del Tolima, Santa Helena Highs, Ibagué-Tolima, 730006299, Colombia; Corresponding author. Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Ibagué-Tolima, 730006299, Colombia.Biomarkers are biological molecules that provide information about the physiological condition of an organism such as early signals of health deterioration. The aim of this study was to compare the relative gene expression of four molecular biomarkers related to oxidative stress, and neuronal damage in Piaractus brachypomus under three different conditions (exposure to chlorpyrifos (CPF), menthol and eugenol anesthesia, and brain injury). Bioinformatic analyses were conducted to design primers for the molecular biomarkers, then, the relative gene expression of COX1, GPX1, BDNF and CALB1 genes was measured by qPCR using brain tissue cDNA from the treated animals as template and 2-ΔΔCT method was performed. EF1a gene was used as reference gene. The results showed a response to chemical stress in the CPF treatment samples where GPX1, BDNF and CALB1 genes were up-regulated. Finally, in menthol/eugenol and brain injury treatments, no alterations in the relative gene expression were observed.http://www.sciencedirect.com/science/article/pii/S2468550X22001435MentholEugenolChlorpyrifosBrain injuryGene expression |
| spellingShingle | Julián David Ortiz Muñoz Kelly Johanna Lozano Villegas Iang Schroniltgen Rondón Barragán Molecular biomarkers for oxidative stress and neuronal damage in red-bellied pacu (Piaractus brachypomus) Aquaculture and Fisheries Menthol Eugenol Chlorpyrifos Brain injury Gene expression |
| title | Molecular biomarkers for oxidative stress and neuronal damage in red-bellied pacu (Piaractus brachypomus) |
| title_full | Molecular biomarkers for oxidative stress and neuronal damage in red-bellied pacu (Piaractus brachypomus) |
| title_fullStr | Molecular biomarkers for oxidative stress and neuronal damage in red-bellied pacu (Piaractus brachypomus) |
| title_full_unstemmed | Molecular biomarkers for oxidative stress and neuronal damage in red-bellied pacu (Piaractus brachypomus) |
| title_short | Molecular biomarkers for oxidative stress and neuronal damage in red-bellied pacu (Piaractus brachypomus) |
| title_sort | molecular biomarkers for oxidative stress and neuronal damage in red bellied pacu piaractus brachypomus |
| topic | Menthol Eugenol Chlorpyrifos Brain injury Gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2468550X22001435 |
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