Selection of reference genes for RT-qPCR analysis in Coccinella septempunctata to assess un-intended effects of RNAi transgenic plants

Selection of reference genes for RT-qPCR analysis in Coccinella septempunctata to assess un-intended effects of RNAi transgenic plants

The development of genetically-engineered plants that employ RNA interference (RNAi) to suppress invertebrate pests opens up new avenues for insect control. While this biotechnology shows tremendous promise, the potential for both non-target and off-target impacts, which likely manifest via altered...

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Bibliographic Details
Main Authors: Chunxiao Yang, Evan L. Preisser, Hongjun Zhang, Yong Liu, Liangying Dai, Huipeng Pan, Xuguo Zhou
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-11-01
Series:Frontiers in Plant Science
Subjects:
Environmental Risk Assessment
reference gene
Coccinella septempunctata
RT-qPCR analysis
Plant incorporated protectant
RNAi-transgenic plants
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01672/full

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