Differential enrichment of bacteria and phages in the vaginal microbiomes in PCOS and obesity: shotgun sequencing analysis
IntroductionPrevious research has linked vaginal bacteria to polycystic ovary syndrome (PCOS) and obesity in women, yet the specific disparities in vaginal microbiota between these conditions remain unclear.MethodsIn this study, we aimed to elucidate the contribution of dysregulated vaginal microbio...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-03-01
|
| Series: | Frontiers in Microbiomes |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/frmbi.2023.1229723/full |
| _version_ | 1797238982537379840 |
|---|---|
| author | Senlin Zheng Huimin Chen Hongyi Yang Xulan Zheng Tengwei Fu Xiaoyan Qiu Meiqin Wang |
| author_facet | Senlin Zheng Huimin Chen Hongyi Yang Xulan Zheng Tengwei Fu Xiaoyan Qiu Meiqin Wang |
| author_sort | Senlin Zheng |
| collection | DOAJ |
| description | IntroductionPrevious research has linked vaginal bacteria to polycystic ovary syndrome (PCOS) and obesity in women, yet the specific disparities in vaginal microbiota between these conditions remain unclear.MethodsIn this study, we aimed to elucidate the contribution of dysregulated vaginal microbiota to PCOS and obesity by analyzing the vaginal microbiota in reproductive-aged women with and without PCOS, as well as obese and non-obese women, using shotgun sequencing.ResultsSwab specimens were collected from four groups of subjects: PCOS and obese, PCOS and non-obese, non-PCOS and obese, and non-PCOS and non-obese. A total of 333 bacteria and 24 viruses/phages were identified to the species level. Clustering analysis revealed that non-PCOS and non-obese individuals exhibit a similar “healthy” vaginal microbiome, while both obesity and PCOS were associated with microbial dysbiosis. Significant differences in abundance were observed for 26 bacterial species and 6 phages/viruses between groups. Notably, pathobionts such as Streptococcus pyogenes, Leptospira santarosai, Citrobacter amalonaticus, Listeria ivanovii, and Clostridium perfringens were significantly less abundant or absent in the non-PCOS and non-obese group. Furthermore, the abundance of Lactobacillus, Pseudomonas bacteria, and their corresponding phages exhibited positive correlations. Lactobacillus bacteria, lactobacillus phage, and pseudomonas phage/virus were identified as indicators of a healthy vaginal microbiome. Importantly, the differentially enriched bacteria in the PCOS and obesity groups were distinct.DiscussionThis study confirms that PCOS and obesity are associated with differing enrichment of bacteria and viruses/phages, with both conditions linked to microbial dysbiosis. Moreover, our findings suggest that vaginal phage diversity is associated with a healthy vaginal microbiota, while dysbiosis is associated with a decrease in phages alongside increased bacterial diversity. |
| first_indexed | 2024-04-24T17:44:18Z |
| format | Article |
| id | doaj.art-c1047f232e84437595e48af6603b6821 |
| institution | Directory Open Access Journal |
| issn | 2813-4338 |
| language | English |
| last_indexed | 2024-04-24T17:44:18Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiomes |
| spelling | doaj.art-c1047f232e84437595e48af6603b68212024-03-27T14:42:32ZengFrontiers Media S.A.Frontiers in Microbiomes2813-43382024-03-01210.3389/frmbi.2023.12297231229723Differential enrichment of bacteria and phages in the vaginal microbiomes in PCOS and obesity: shotgun sequencing analysisSenlin Zheng0Huimin Chen1Hongyi Yang2Xulan Zheng3Tengwei Fu4Xiaoyan Qiu5Meiqin Wang6Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaDepartment of Gynecology and Obstetrics, The First Affiliated Hospital of Xiamen University, Xiamen, ChinaDepartment of Gynecology and Obstetrics, The First Affiliated Hospital of Xiamen University, Xiamen, ChinaDepartment of Psychology, University of British Columbia, Vancouver, BC, CanadaYanxuan Biotech Ltd., Xiamen, ChinaYanxuan Biotech Ltd., Xiamen, ChinaDepartment of Gynecology and Obstetrics, The First Affiliated Hospital of Xiamen University, Xiamen, ChinaIntroductionPrevious research has linked vaginal bacteria to polycystic ovary syndrome (PCOS) and obesity in women, yet the specific disparities in vaginal microbiota between these conditions remain unclear.MethodsIn this study, we aimed to elucidate the contribution of dysregulated vaginal microbiota to PCOS and obesity by analyzing the vaginal microbiota in reproductive-aged women with and without PCOS, as well as obese and non-obese women, using shotgun sequencing.ResultsSwab specimens were collected from four groups of subjects: PCOS and obese, PCOS and non-obese, non-PCOS and obese, and non-PCOS and non-obese. A total of 333 bacteria and 24 viruses/phages were identified to the species level. Clustering analysis revealed that non-PCOS and non-obese individuals exhibit a similar “healthy” vaginal microbiome, while both obesity and PCOS were associated with microbial dysbiosis. Significant differences in abundance were observed for 26 bacterial species and 6 phages/viruses between groups. Notably, pathobionts such as Streptococcus pyogenes, Leptospira santarosai, Citrobacter amalonaticus, Listeria ivanovii, and Clostridium perfringens were significantly less abundant or absent in the non-PCOS and non-obese group. Furthermore, the abundance of Lactobacillus, Pseudomonas bacteria, and their corresponding phages exhibited positive correlations. Lactobacillus bacteria, lactobacillus phage, and pseudomonas phage/virus were identified as indicators of a healthy vaginal microbiome. Importantly, the differentially enriched bacteria in the PCOS and obesity groups were distinct.DiscussionThis study confirms that PCOS and obesity are associated with differing enrichment of bacteria and viruses/phages, with both conditions linked to microbial dysbiosis. Moreover, our findings suggest that vaginal phage diversity is associated with a healthy vaginal microbiota, while dysbiosis is associated with a decrease in phages alongside increased bacterial diversity.https://www.frontiersin.org/articles/10.3389/frmbi.2023.1229723/fullvaginal microbiomepolycystic ovary syndromeobesitybacteriaphages |
| spellingShingle | Senlin Zheng Huimin Chen Hongyi Yang Xulan Zheng Tengwei Fu Xiaoyan Qiu Meiqin Wang Differential enrichment of bacteria and phages in the vaginal microbiomes in PCOS and obesity: shotgun sequencing analysis Frontiers in Microbiomes vaginal microbiome polycystic ovary syndrome obesity bacteria phages |
| title | Differential enrichment of bacteria and phages in the vaginal microbiomes in PCOS and obesity: shotgun sequencing analysis |
| title_full | Differential enrichment of bacteria and phages in the vaginal microbiomes in PCOS and obesity: shotgun sequencing analysis |
| title_fullStr | Differential enrichment of bacteria and phages in the vaginal microbiomes in PCOS and obesity: shotgun sequencing analysis |
| title_full_unstemmed | Differential enrichment of bacteria and phages in the vaginal microbiomes in PCOS and obesity: shotgun sequencing analysis |
| title_short | Differential enrichment of bacteria and phages in the vaginal microbiomes in PCOS and obesity: shotgun sequencing analysis |
| title_sort | differential enrichment of bacteria and phages in the vaginal microbiomes in pcos and obesity shotgun sequencing analysis |
| topic | vaginal microbiome polycystic ovary syndrome obesity bacteria phages |
| url | https://www.frontiersin.org/articles/10.3389/frmbi.2023.1229723/full |
| work_keys_str_mv | AT senlinzheng differentialenrichmentofbacteriaandphagesinthevaginalmicrobiomesinpcosandobesityshotgunsequencinganalysis AT huiminchen differentialenrichmentofbacteriaandphagesinthevaginalmicrobiomesinpcosandobesityshotgunsequencinganalysis AT hongyiyang differentialenrichmentofbacteriaandphagesinthevaginalmicrobiomesinpcosandobesityshotgunsequencinganalysis AT xulanzheng differentialenrichmentofbacteriaandphagesinthevaginalmicrobiomesinpcosandobesityshotgunsequencinganalysis AT tengweifu differentialenrichmentofbacteriaandphagesinthevaginalmicrobiomesinpcosandobesityshotgunsequencinganalysis AT xiaoyanqiu differentialenrichmentofbacteriaandphagesinthevaginalmicrobiomesinpcosandobesityshotgunsequencinganalysis AT meiqinwang differentialenrichmentofbacteriaandphagesinthevaginalmicrobiomesinpcosandobesityshotgunsequencinganalysis |