Effects of <i>Rhodiola sachalinensis</i> on ocular blood flow in diabetic retinopathy rats
AIM: To investigate the effects of <i>Rhodiola sachalinensis</i> on ocular blood flow in diabetic retinopathy rats. <p>METHODS: A total of 90 SD rats were randomly divided into control group(<i>n</i>=30), model group(<i>n</i>=30)and intervention group(<i&...
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Press of International Journal of Ophthalmology (IJO PRESS)
2018-05-01
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Series: | Guoji Yanke Zazhi |
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Online Access: | http://ies.ijo.cn/cn_publish/2018/5/201805005.pdf |
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author | Ai-Ping Gu Yi Wu Bing-Hua Yang |
author_facet | Ai-Ping Gu Yi Wu Bing-Hua Yang |
author_sort | Ai-Ping Gu |
collection | DOAJ |
description | AIM: To investigate the effects of <i>Rhodiola sachalinensis</i> on ocular blood flow in diabetic retinopathy rats. <p>METHODS: A total of 90 SD rats were randomly divided into control group(<i>n</i>=30), model group(<i>n</i>=30)and intervention group(<i>n</i>=30). Rats in the model group and intervention group were fed with high glucose and high fat diet and injected with streptozotocin(40mg/kg)in order to construct DR model rats, while rats in the control group were fed with basic diet and injected with the same amount of normal saline. After 4wk, the rats in the intervention group were injected with <i>Rhodiola sachalinensis</i> injection(10mL, one per day), while rats in the control group and the model group were injected with normal saline. The course of intervention treatment was 4wk. The ocular blood flow in rats was detected by laser doppler flowmetry, the apoptosis of retinal cells in rats was detected by TUNEL method, the expression of vascular endothelial growth factor(VEGF), glial fibrillary acidic protein(GFAP)and glutamate/aspartate transporter(GLAST)in retina of rats was detected by RT-PCR method and Western blot method. <p>RESULTS: The whole blood viscosity, whole blood high shear viscosity, low shear blood viscosity and erythrocyte sedimentation of rats in the model group and intervention group were higher than those in the control group(<i>P</i><0.01), while the indexes of intervention group were lower than that of model group(<i>P</i><0.01). The PSV and EDC of rats in the model group and intervention group were lower than those in the control group(<i>P</i><0.01), and the RI was higher than that in control group(<i>P</i><0.01). The PSV and EDC in the intervention group were higher than those in the model group(<i>P</i><0.01), and RI was lower than that in the model group(<i>P</i><0.01). The apoptosis rate of retinal cells of model group and intervention group was higher than that of the control group(<i>P</i><0.01), that in the intervention group was lower than that in the model group(<i>P</i><0.01). The expression of VEGF and GFAP in retina tissue of model group and intervention group was higher than that of the control group(<i>P</i><0.01), and the expression of GLAST was lower than that in the control group(<i>P</i><0.01). The expression of VEGF and GFAP in the intervention group was lower than that in the model group(<i>P</i><0.01), and the expression of GLAST was higher than that in the model group(<i>P</i><0.01). <p>CONCLUSION: <i>Rhodiola sachalinensis</i> injection can significantly improve the ocular blood flow in diabetic retinopathy rats, reduce the apoptosis of retinal cells in rats, down regulate the expression of VEGF and GFAP, and up regulate the expression of GLAST. |
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issn | 1672-5123 1672-5123 |
language | English |
last_indexed | 2024-04-12T17:45:33Z |
publishDate | 2018-05-01 |
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series | Guoji Yanke Zazhi |
spelling | doaj.art-c1049540fcc94643870087f5c7d7f6ec2022-12-22T03:22:41ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232018-05-0118579179510.3980/j.issn.1672-5123.2018.5.05Effects of <i>Rhodiola sachalinensis</i> on ocular blood flow in diabetic retinopathy ratsAi-Ping Gu0Yi Wu1Bing-Hua Yang2Department of Ophthalmology, the Guangdong Second Provincial General Hospital, Guangzhou 510317, Guangdong Province, ChinaDepartment of Ophthalmology, the Guangdong Second Provincial General Hospital, Guangzhou 510317, Guangdong Province, ChinaDepartment of Ophthalmology, the Guangdong Second Provincial General Hospital, Guangzhou 510317, Guangdong Province, ChinaAIM: To investigate the effects of <i>Rhodiola sachalinensis</i> on ocular blood flow in diabetic retinopathy rats. <p>METHODS: A total of 90 SD rats were randomly divided into control group(<i>n</i>=30), model group(<i>n</i>=30)and intervention group(<i>n</i>=30). Rats in the model group and intervention group were fed with high glucose and high fat diet and injected with streptozotocin(40mg/kg)in order to construct DR model rats, while rats in the control group were fed with basic diet and injected with the same amount of normal saline. After 4wk, the rats in the intervention group were injected with <i>Rhodiola sachalinensis</i> injection(10mL, one per day), while rats in the control group and the model group were injected with normal saline. The course of intervention treatment was 4wk. The ocular blood flow in rats was detected by laser doppler flowmetry, the apoptosis of retinal cells in rats was detected by TUNEL method, the expression of vascular endothelial growth factor(VEGF), glial fibrillary acidic protein(GFAP)and glutamate/aspartate transporter(GLAST)in retina of rats was detected by RT-PCR method and Western blot method. <p>RESULTS: The whole blood viscosity, whole blood high shear viscosity, low shear blood viscosity and erythrocyte sedimentation of rats in the model group and intervention group were higher than those in the control group(<i>P</i><0.01), while the indexes of intervention group were lower than that of model group(<i>P</i><0.01). The PSV and EDC of rats in the model group and intervention group were lower than those in the control group(<i>P</i><0.01), and the RI was higher than that in control group(<i>P</i><0.01). The PSV and EDC in the intervention group were higher than those in the model group(<i>P</i><0.01), and RI was lower than that in the model group(<i>P</i><0.01). The apoptosis rate of retinal cells of model group and intervention group was higher than that of the control group(<i>P</i><0.01), that in the intervention group was lower than that in the model group(<i>P</i><0.01). The expression of VEGF and GFAP in retina tissue of model group and intervention group was higher than that of the control group(<i>P</i><0.01), and the expression of GLAST was lower than that in the control group(<i>P</i><0.01). The expression of VEGF and GFAP in the intervention group was lower than that in the model group(<i>P</i><0.01), and the expression of GLAST was higher than that in the model group(<i>P</i><0.01). <p>CONCLUSION: <i>Rhodiola sachalinensis</i> injection can significantly improve the ocular blood flow in diabetic retinopathy rats, reduce the apoptosis of retinal cells in rats, down regulate the expression of VEGF and GFAP, and up regulate the expression of GLAST.http://ies.ijo.cn/cn_publish/2018/5/201805005.pdf<i>Rhodiola sachalinensis</i>diabetic retinopathyhemorheologyhemodynamicshemodynamics |
spellingShingle | Ai-Ping Gu Yi Wu Bing-Hua Yang Effects of <i>Rhodiola sachalinensis</i> on ocular blood flow in diabetic retinopathy rats Guoji Yanke Zazhi <i>Rhodiola sachalinensis</i> diabetic retinopathy hemorheology hemodynamics hemodynamics |
title | Effects of <i>Rhodiola sachalinensis</i> on ocular blood flow in diabetic retinopathy rats |
title_full | Effects of <i>Rhodiola sachalinensis</i> on ocular blood flow in diabetic retinopathy rats |
title_fullStr | Effects of <i>Rhodiola sachalinensis</i> on ocular blood flow in diabetic retinopathy rats |
title_full_unstemmed | Effects of <i>Rhodiola sachalinensis</i> on ocular blood flow in diabetic retinopathy rats |
title_short | Effects of <i>Rhodiola sachalinensis</i> on ocular blood flow in diabetic retinopathy rats |
title_sort | effects of i rhodiola sachalinensis i on ocular blood flow in diabetic retinopathy rats |
topic | <i>Rhodiola sachalinensis</i> diabetic retinopathy hemorheology hemodynamics hemodynamics |
url | http://ies.ijo.cn/cn_publish/2018/5/201805005.pdf |
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