Chemical Diversity and Antimicrobial Potential of Cultivable Fungi from Deep-Sea Sediments of the Gulf of Mexico
A collection of 29 cultivable fungal strains isolated from deep-sea sediments of the Gulf of Mexico were cultivated under the “one strain, many compounds” approach to explore their chemical diversity and antimicrobial potential. From the 87 extracts tested, over 50% showed antimicrobial activity, an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/23/7328 |
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| author | Rodrigo Villanueva-Silva Patricia Velez Meritxell Riquelme Carlos A. Fajardo-Hernández Anahí Martínez-Cárdenas Alejandra Arista-Romero Baojie Wan Rui Ma Mallique Qader Scott G. Franzblau Mario Figueroa |
| author_facet | Rodrigo Villanueva-Silva Patricia Velez Meritxell Riquelme Carlos A. Fajardo-Hernández Anahí Martínez-Cárdenas Alejandra Arista-Romero Baojie Wan Rui Ma Mallique Qader Scott G. Franzblau Mario Figueroa |
| author_sort | Rodrigo Villanueva-Silva |
| collection | DOAJ |
| description | A collection of 29 cultivable fungal strains isolated from deep-sea sediments of the Gulf of Mexico were cultivated under the “one strain, many compounds” approach to explore their chemical diversity and antimicrobial potential. From the 87 extracts tested, over 50% showed antimicrobial activity, and the most active ones were those from cultures grown at 4 °C in darkness for 60 days (resembling deep-sea temperature). PCA analysis of the LC-MS data of all the extracts confirmed that culture temperature is the primary factor in the variation of the 4462 metabolite features, accounting for 21.3% of the variation. The bioactivity-guided and conventional chemical studies of selected fungal strains allowed the identification of several active and specialized metabolites. Finally, metabolomics analysis by GNPS molecular networking and manual dereplication revealed the biosynthetic potential of these species to produce interesting chemistry. This work uncovers the chemical and biological study of marine-derived fungal strains from deep-sea sediments of the Gulf of Mexico. |
| first_indexed | 2024-03-10T04:47:42Z |
| format | Article |
| id | doaj.art-c105c9abdf6d4a3abbf095ff70895b8d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T04:47:42Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c105c9abdf6d4a3abbf095ff70895b8d2023-11-23T02:50:49ZengMDPI AGMolecules1420-30492021-12-012623732810.3390/molecules26237328Chemical Diversity and Antimicrobial Potential of Cultivable Fungi from Deep-Sea Sediments of the Gulf of MexicoRodrigo Villanueva-Silva0Patricia Velez1Meritxell Riquelme2Carlos A. Fajardo-Hernández3Anahí Martínez-Cárdenas4Alejandra Arista-Romero5Baojie Wan6Rui Ma7Mallique Qader8Scott G. Franzblau9Mario Figueroa10Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoInstituto de Biología, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoDepartamento de Microbiología, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Ensenada, Baja California 22860, MexicoFacultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoFacultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoFacultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoInstitute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USAInstitute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USAInstitute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USAInstitute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USAFacultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoA collection of 29 cultivable fungal strains isolated from deep-sea sediments of the Gulf of Mexico were cultivated under the “one strain, many compounds” approach to explore their chemical diversity and antimicrobial potential. From the 87 extracts tested, over 50% showed antimicrobial activity, and the most active ones were those from cultures grown at 4 °C in darkness for 60 days (resembling deep-sea temperature). PCA analysis of the LC-MS data of all the extracts confirmed that culture temperature is the primary factor in the variation of the 4462 metabolite features, accounting for 21.3% of the variation. The bioactivity-guided and conventional chemical studies of selected fungal strains allowed the identification of several active and specialized metabolites. Finally, metabolomics analysis by GNPS molecular networking and manual dereplication revealed the biosynthetic potential of these species to produce interesting chemistry. This work uncovers the chemical and biological study of marine-derived fungal strains from deep-sea sediments of the Gulf of Mexico.https://www.mdpi.com/1420-3049/26/23/7328marine fungideep-sea sedimentschemical diversitymetabolomicsantimicrobial activity |
| spellingShingle | Rodrigo Villanueva-Silva Patricia Velez Meritxell Riquelme Carlos A. Fajardo-Hernández Anahí Martínez-Cárdenas Alejandra Arista-Romero Baojie Wan Rui Ma Mallique Qader Scott G. Franzblau Mario Figueroa Chemical Diversity and Antimicrobial Potential of Cultivable Fungi from Deep-Sea Sediments of the Gulf of Mexico Molecules marine fungi deep-sea sediments chemical diversity metabolomics antimicrobial activity |
| title | Chemical Diversity and Antimicrobial Potential of Cultivable Fungi from Deep-Sea Sediments of the Gulf of Mexico |
| title_full | Chemical Diversity and Antimicrobial Potential of Cultivable Fungi from Deep-Sea Sediments of the Gulf of Mexico |
| title_fullStr | Chemical Diversity and Antimicrobial Potential of Cultivable Fungi from Deep-Sea Sediments of the Gulf of Mexico |
| title_full_unstemmed | Chemical Diversity and Antimicrobial Potential of Cultivable Fungi from Deep-Sea Sediments of the Gulf of Mexico |
| title_short | Chemical Diversity and Antimicrobial Potential of Cultivable Fungi from Deep-Sea Sediments of the Gulf of Mexico |
| title_sort | chemical diversity and antimicrobial potential of cultivable fungi from deep sea sediments of the gulf of mexico |
| topic | marine fungi deep-sea sediments chemical diversity metabolomics antimicrobial activity |
| url | https://www.mdpi.com/1420-3049/26/23/7328 |
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