Supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryos
The transition period of dairy cows is characterized by several metabolic alterations, due to the negative energy balance, which negatively affects fertility. The aim of this study was to evaluate the effect of supplementing the in vitro maturation medium with serum from dairy cows at early and late...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal Rural de Pernambuco
2019-04-01
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| Series: | Medicina Veterinária |
| Subjects: | |
| Online Access: | https://journals.ufrpe.br/index.php/medicinaveterinaria/article/view/2462 |
| _version_ | 1797776819971162112 |
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| author | Letícia Franco Collares Jorgea Pradieé Morgana Alves Borges Bruna Mion Patrícia Gindri Janaína Fadrique Joao Alveiro Alvarado Rincón Lígia Margareth Cantarelli Pegoraro Augusto Schneider Marcio Nunes Corrêa |
| author_facet | Letícia Franco Collares Jorgea Pradieé Morgana Alves Borges Bruna Mion Patrícia Gindri Janaína Fadrique Joao Alveiro Alvarado Rincón Lígia Margareth Cantarelli Pegoraro Augusto Schneider Marcio Nunes Corrêa |
| author_sort | Letícia Franco Collares |
| collection | DOAJ |
| description | The transition period of dairy cows is characterized by several metabolic alterations, due to the negative energy balance, which negatively affects fertility. The aim of this study was to evaluate the effect of supplementing the in vitro maturation medium with serum from dairy cows at early and late lactation in the oocyte maturation on the rates of nuclear maturation, cleavage and initial embryo development. The cumulus-oocyte complexes (COCs) were randomly divided into treatments: LL: serum from four cows at 354 ± 3.8 days in milk (DIM); and EL: serum from four cows at 10.8 ± 4.8 DIM. Subsequently, the COCs were submitted to maturation, fertilization; and in vitro culture in an incubator at 39°C with 5% CO2. For analysis of nuclear maturation; oocytes were collected after IVM, denuded and stained with Hoechst®. No difference was observed in the number of oocytes that reached metaphase II among treatments. The cleavage rate was not different between LL (59.3%; 320/540) and EL (47.0%; 243/517) treatments (P>0.05), as embryonic development between LL (15.0%; 81/540) and EL (16.6%; 86/517) (P>0.05). It was concluded that supplementation with serum from cows at different lactation periods during the bovine oocyte IVM did not influence the results of in vitro embryo production. |
| first_indexed | 2024-03-12T22:55:18Z |
| format | Article |
| id | doaj.art-c10614e4116642cc932603201d1b6056 |
| institution | Directory Open Access Journal |
| issn | 1809-4678 2675-6617 |
| language | English |
| last_indexed | 2024-03-12T22:55:18Z |
| publishDate | 2019-04-01 |
| publisher | Universidade Federal Rural de Pernambuco |
| record_format | Article |
| series | Medicina Veterinária |
| spelling | doaj.art-c10614e4116642cc932603201d1b60562023-07-20T00:16:12ZengUniversidade Federal Rural de PernambucoMedicina Veterinária1809-46782675-66172019-04-01124Supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryosLetícia Franco Collares0Jorgea Pradieé1Morgana Alves Borges2Bruna Mion3Patrícia Gindri4Janaína Fadrique5Joao Alveiro Alvarado Rincón6Lígia Margareth Cantarelli Pegoraro7Augusto Schneider8Marcio Nunes Corrêa9Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.Empresa Brasileira de Pesquisa Agropecuária, Embrapa Clima Temperado, Pelotas-RS, Brasil.Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.The transition period of dairy cows is characterized by several metabolic alterations, due to the negative energy balance, which negatively affects fertility. The aim of this study was to evaluate the effect of supplementing the in vitro maturation medium with serum from dairy cows at early and late lactation in the oocyte maturation on the rates of nuclear maturation, cleavage and initial embryo development. The cumulus-oocyte complexes (COCs) were randomly divided into treatments: LL: serum from four cows at 354 ± 3.8 days in milk (DIM); and EL: serum from four cows at 10.8 ± 4.8 DIM. Subsequently, the COCs were submitted to maturation, fertilization; and in vitro culture in an incubator at 39°C with 5% CO2. For analysis of nuclear maturation; oocytes were collected after IVM, denuded and stained with Hoechst®. No difference was observed in the number of oocytes that reached metaphase II among treatments. The cleavage rate was not different between LL (59.3%; 320/540) and EL (47.0%; 243/517) treatments (P>0.05), as embryonic development between LL (15.0%; 81/540) and EL (16.6%; 86/517) (P>0.05). It was concluded that supplementation with serum from cows at different lactation periods during the bovine oocyte IVM did not influence the results of in vitro embryo production.https://journals.ufrpe.br/index.php/medicinaveterinaria/article/view/2462metabolismoocyte maturationtransition period. |
| spellingShingle | Letícia Franco Collares Jorgea Pradieé Morgana Alves Borges Bruna Mion Patrícia Gindri Janaína Fadrique Joao Alveiro Alvarado Rincón Lígia Margareth Cantarelli Pegoraro Augusto Schneider Marcio Nunes Corrêa Supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryos Medicina Veterinária metabolism oocyte maturation transition period. |
| title | Supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryos |
| title_full | Supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryos |
| title_fullStr | Supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryos |
| title_full_unstemmed | Supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryos |
| title_short | Supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryos |
| title_sort | supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryos |
| topic | metabolism oocyte maturation transition period. |
| url | https://journals.ufrpe.br/index.php/medicinaveterinaria/article/view/2462 |
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