Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)

Eighty-one lines of cauliflower (Brassica oleracea var. botrytis) from 12 populations used to produce commercial hybrids in Brazil were screened for polymorphism in the acid phosphatase system, in order to evaluate the usefulness of this marker for the determination of the parental contamination lev...

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Main Authors: Celso Luis Marino, Marcos Aparecido Gimenes, Norberto da Silva, Catalina Romero Lopes
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2002-01-01
Series:Genetics and Molecular Biology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100015
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author Celso Luis Marino
Marcos Aparecido Gimenes
Norberto da Silva
Catalina Romero Lopes
author_facet Celso Luis Marino
Marcos Aparecido Gimenes
Norberto da Silva
Catalina Romero Lopes
author_sort Celso Luis Marino
collection DOAJ
description Eighty-one lines of cauliflower (Brassica oleracea var. botrytis) from 12 populations used to produce commercial hybrids in Brazil were screened for polymorphism in the acid phosphatase system, in order to evaluate the usefulness of this marker for the determination of the parental contamination level in hybrid seeds. Little polymorphism was detected in the examined lines, but the system appeared to be very useful for hybrid identification, since the only condition required was polymorphism between the two parental lines. If the analyzed lines were used for hybrid production, 8.4% and 12.3% of the possible crosses would result in hybrids which can be positively identified using the APS-1 and B1 loci, respectively. If only one plant of each homozygous type (SS or FF) was analyzed in each population, 41% and 50% of the possible crosses would result in hybrids which can be positively identified using the APS-1 and B1 loci, respectively.
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spelling doaj.art-c1067725aae14dd0b9a8a7aae3b7135d2022-12-22T03:44:34ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852002-01-01251818410.1590/S1415-47572002000100015Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)Celso Luis MarinoMarcos Aparecido GimenesNorberto da SilvaCatalina Romero LopesEighty-one lines of cauliflower (Brassica oleracea var. botrytis) from 12 populations used to produce commercial hybrids in Brazil were screened for polymorphism in the acid phosphatase system, in order to evaluate the usefulness of this marker for the determination of the parental contamination level in hybrid seeds. Little polymorphism was detected in the examined lines, but the system appeared to be very useful for hybrid identification, since the only condition required was polymorphism between the two parental lines. If the analyzed lines were used for hybrid production, 8.4% and 12.3% of the possible crosses would result in hybrids which can be positively identified using the APS-1 and B1 loci, respectively. If only one plant of each homozygous type (SS or FF) was analyzed in each population, 41% and 50% of the possible crosses would result in hybrids which can be positively identified using the APS-1 and B1 loci, respectively.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100015isoenzymepolymorphismcauliflower
spellingShingle Celso Luis Marino
Marcos Aparecido Gimenes
Norberto da Silva
Catalina Romero Lopes
Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)
Genetics and Molecular Biology
isoenzyme
polymorphism
cauliflower
title Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)
title_full Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)
title_fullStr Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)
title_full_unstemmed Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)
title_short Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)
title_sort acid phosphatase polymorphism within and among populations of cauliflower brassica oleracea var botrytis
topic isoenzyme
polymorphism
cauliflower
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100015
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AT norbertodasilva acidphosphatasepolymorphismwithinandamongpopulationsofcauliflowerbrassicaoleraceavarbotrytis
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