Detection and multilocus genotyping of Giardia duodenalis in dogs in Sichuan province, China
Giardia duodenalis (also known as G. intestinalis) is a flagellated protozoan that parasitizes the small intestine and is a common causal agent of zoonotic infections in humans and animals. To assess the genetic diversity and zoonotic transmission potential of G. duodenalis in stray dogs, 159 fecal...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2017-01-01
|
| Series: | Parasite |
| Subjects: | |
| Online Access: | https://doi.org/10.1051/parasite/2017032 |
| _version_ | 1797423313563156480 |
|---|---|
| author | Zhang Yue Zhong Zhijun Deng Lei Wang Maoqing Li Wei Gong Chao Fu Hualin Cao Suizhong Shi Xianpeng Wu Kongju Peng Guangneng |
| author_facet | Zhang Yue Zhong Zhijun Deng Lei Wang Maoqing Li Wei Gong Chao Fu Hualin Cao Suizhong Shi Xianpeng Wu Kongju Peng Guangneng |
| author_sort | Zhang Yue |
| collection | DOAJ |
| description | Giardia duodenalis (also known as G. intestinalis) is a flagellated protozoan that parasitizes the small intestine and is a common causal agent of zoonotic infections in humans and animals. To assess the genetic diversity and zoonotic transmission potential of G. duodenalis in stray dogs, 159 fecal specimens were collected from dogs in Chengdu, Yaan, and Leshan in Sichuan province, China. Of the 159 fecal samples from stray dogs, 18 (11.3%) were G. duodenalis-positive based on nested PCR amplification of the beta giardin (bg) gene, and the occurrence varied from 1.8% to 35% in different cities. Dog-specific assemblages C (n = 9) and D (n = 9) were identified. The glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi) genes of all bg-positive isolates were characterized. A total of 16 and 8 isolates were positive for the gdh and tpi genes, respectively. Two novel sequences of the bg locus were detected among genetic assemblage D isolates, and one novel gdh sequence and four novel tpi sequences were identified among genetic assemblage C isolates. Mixed infections of assemblages C and D were also detected. Assemblages A and B, which have high zoonotic potential, were not detected. Our results show that G. duodenalis is prevalent and a cause of diarrhea in dogs in Sichuan province, China. |
| first_indexed | 2024-03-09T07:46:03Z |
| format | Article |
| id | doaj.art-c108931e157b4ba4ab60ba3b73049cba |
| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-09T07:46:03Z |
| publishDate | 2017-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-c108931e157b4ba4ab60ba3b73049cba2023-12-03T03:35:26ZengEDP SciencesParasite1776-10422017-01-01243110.1051/parasite/2017032parasite170061Detection and multilocus genotyping of Giardia duodenalis in dogs in Sichuan province, ChinaZhang YueZhong ZhijunDeng LeiWang MaoqingLi WeiGong ChaoFu HualinCao SuizhongShi XianpengWu KongjuPeng GuangnengGiardia duodenalis (also known as G. intestinalis) is a flagellated protozoan that parasitizes the small intestine and is a common causal agent of zoonotic infections in humans and animals. To assess the genetic diversity and zoonotic transmission potential of G. duodenalis in stray dogs, 159 fecal specimens were collected from dogs in Chengdu, Yaan, and Leshan in Sichuan province, China. Of the 159 fecal samples from stray dogs, 18 (11.3%) were G. duodenalis-positive based on nested PCR amplification of the beta giardin (bg) gene, and the occurrence varied from 1.8% to 35% in different cities. Dog-specific assemblages C (n = 9) and D (n = 9) were identified. The glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi) genes of all bg-positive isolates were characterized. A total of 16 and 8 isolates were positive for the gdh and tpi genes, respectively. Two novel sequences of the bg locus were detected among genetic assemblage D isolates, and one novel gdh sequence and four novel tpi sequences were identified among genetic assemblage C isolates. Mixed infections of assemblages C and D were also detected. Assemblages A and B, which have high zoonotic potential, were not detected. Our results show that G. duodenalis is prevalent and a cause of diarrhea in dogs in Sichuan province, China.https://doi.org/10.1051/parasite/2017032GiardiaProtozoanDogChinaPCR |
| spellingShingle | Zhang Yue Zhong Zhijun Deng Lei Wang Maoqing Li Wei Gong Chao Fu Hualin Cao Suizhong Shi Xianpeng Wu Kongju Peng Guangneng Detection and multilocus genotyping of Giardia duodenalis in dogs in Sichuan province, China Parasite Giardia Protozoan Dog China PCR |
| title | Detection and multilocus genotyping of Giardia duodenalis in dogs in Sichuan province, China |
| title_full | Detection and multilocus genotyping of Giardia duodenalis in dogs in Sichuan province, China |
| title_fullStr | Detection and multilocus genotyping of Giardia duodenalis in dogs in Sichuan province, China |
| title_full_unstemmed | Detection and multilocus genotyping of Giardia duodenalis in dogs in Sichuan province, China |
| title_short | Detection and multilocus genotyping of Giardia duodenalis in dogs in Sichuan province, China |
| title_sort | detection and multilocus genotyping of giardia duodenalis in dogs in sichuan province china |
| topic | Giardia Protozoan Dog China PCR |
| url | https://doi.org/10.1051/parasite/2017032 |
| work_keys_str_mv | AT zhangyue detectionandmultilocusgenotypingofgiardiaduodenalisindogsinsichuanprovincechina AT zhongzhijun detectionandmultilocusgenotypingofgiardiaduodenalisindogsinsichuanprovincechina AT denglei detectionandmultilocusgenotypingofgiardiaduodenalisindogsinsichuanprovincechina AT wangmaoqing detectionandmultilocusgenotypingofgiardiaduodenalisindogsinsichuanprovincechina AT liwei detectionandmultilocusgenotypingofgiardiaduodenalisindogsinsichuanprovincechina AT gongchao detectionandmultilocusgenotypingofgiardiaduodenalisindogsinsichuanprovincechina AT fuhualin detectionandmultilocusgenotypingofgiardiaduodenalisindogsinsichuanprovincechina AT caosuizhong detectionandmultilocusgenotypingofgiardiaduodenalisindogsinsichuanprovincechina AT shixianpeng detectionandmultilocusgenotypingofgiardiaduodenalisindogsinsichuanprovincechina AT wukongju detectionandmultilocusgenotypingofgiardiaduodenalisindogsinsichuanprovincechina AT pengguangneng detectionandmultilocusgenotypingofgiardiaduodenalisindogsinsichuanprovincechina |