Effect of milk exposure on the redox profile of Caenorhabditis elegans
Abstract The consumption of bovine milk and its derivatives is associated with inflammation, gastrointestinal disorders and the development of diseases in humans. Most studies related to milk effects are based on either clinal trials or experimental models such as mice and cell cultures. In this stu...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-01-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52254-6 |
| _version_ | 1797276569618612224 |
|---|---|
| author | Ingrid Laíse Silvestre de Oliveira Giovanna Melo Martins Silva Cesar Orlando Muñoz Cadavid Danielle Cavalcanti Sales Katya Anaya Riva de Paula Oliveira Adriano Henrique do Nascimento Rangel |
| author_facet | Ingrid Laíse Silvestre de Oliveira Giovanna Melo Martins Silva Cesar Orlando Muñoz Cadavid Danielle Cavalcanti Sales Katya Anaya Riva de Paula Oliveira Adriano Henrique do Nascimento Rangel |
| author_sort | Ingrid Laíse Silvestre de Oliveira |
| collection | DOAJ |
| description | Abstract The consumption of bovine milk and its derivatives is associated with inflammation, gastrointestinal disorders and the development of diseases in humans. Most studies related to milk effects are based on either clinal trials or experimental models such as mice and cell cultures. In this study we present the nematode Caenorhabditis elegans as an alternative model to evaluate the effects of milk on oxidative stress in other animal models. The toxicological effect of 20% milk exposure for 8 h on C. elegans was evaluated by progeny quantification, body size and pharyngeal pumping rate. Treating the worms with milk did not affect the worms brood size but interfered with their fecundity by delaying the average number of eggs in the first day of oviposition when compared to the control group. The size of worms treated with milk were significantly smaller compared to control. The pharyngeal pumping rate of milk-treated animals was not significantly different compared to untreated animals. Taking together, the results suggest that 20% milk treatment is not toxic for the worms but induces a minor delay achieving its adulthood and therefore its reproduction period. Milk exposure did not reduce the worms’ survival under stress conditions and increase endogenous ROS levels. This study contributes to characterize the effects of milk exposure on the C. elegans nematode. |
| first_indexed | 2024-03-07T15:30:03Z |
| format | Article |
| id | doaj.art-c10df863667a4ce1ae26f1eecba2ba12 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:30:03Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c10df863667a4ce1ae26f1eecba2ba122024-03-05T16:29:29ZengNature PortfolioScientific Reports2045-23222024-01-011411610.1038/s41598-024-52254-6Effect of milk exposure on the redox profile of Caenorhabditis elegansIngrid Laíse Silvestre de Oliveira0Giovanna Melo Martins Silva1Cesar Orlando Muñoz Cadavid2Danielle Cavalcanti Sales3Katya Anaya4Riva de Paula Oliveira5Adriano Henrique do Nascimento Rangel6Escola Agrícola de Jundiaí, Unidade Acadêmica Especializada em Ciências Agrárias, Universidade Federal do Rio Grande do NorteRede Nordeste de Biotecnologia (RENORBIO), Universidade Federal do Rio Grande do NorteRede Nordeste de Biotecnologia (RENORBIO), Universidade Federal do Rio Grande do NorteEscola Agrícola de Jundiaí, Unidade Acadêmica Especializada em Ciências Agrárias, Universidade Federal do Rio Grande do NorteFaculdade de Ciências da Saúde Do Trairí (FACISA), Universidade Federal do Rio Grande do NorteDepartamento de Biologia Celular E Genética, Universidade Federal do Rio Grande do NorteEscola Agrícola de Jundiaí, Unidade Acadêmica Especializada em Ciências Agrárias, Universidade Federal do Rio Grande do NorteAbstract The consumption of bovine milk and its derivatives is associated with inflammation, gastrointestinal disorders and the development of diseases in humans. Most studies related to milk effects are based on either clinal trials or experimental models such as mice and cell cultures. In this study we present the nematode Caenorhabditis elegans as an alternative model to evaluate the effects of milk on oxidative stress in other animal models. The toxicological effect of 20% milk exposure for 8 h on C. elegans was evaluated by progeny quantification, body size and pharyngeal pumping rate. Treating the worms with milk did not affect the worms brood size but interfered with their fecundity by delaying the average number of eggs in the first day of oviposition when compared to the control group. The size of worms treated with milk were significantly smaller compared to control. The pharyngeal pumping rate of milk-treated animals was not significantly different compared to untreated animals. Taking together, the results suggest that 20% milk treatment is not toxic for the worms but induces a minor delay achieving its adulthood and therefore its reproduction period. Milk exposure did not reduce the worms’ survival under stress conditions and increase endogenous ROS levels. This study contributes to characterize the effects of milk exposure on the C. elegans nematode.https://doi.org/10.1038/s41598-024-52254-6 |
| spellingShingle | Ingrid Laíse Silvestre de Oliveira Giovanna Melo Martins Silva Cesar Orlando Muñoz Cadavid Danielle Cavalcanti Sales Katya Anaya Riva de Paula Oliveira Adriano Henrique do Nascimento Rangel Effect of milk exposure on the redox profile of Caenorhabditis elegans Scientific Reports |
| title | Effect of milk exposure on the redox profile of Caenorhabditis elegans |
| title_full | Effect of milk exposure on the redox profile of Caenorhabditis elegans |
| title_fullStr | Effect of milk exposure on the redox profile of Caenorhabditis elegans |
| title_full_unstemmed | Effect of milk exposure on the redox profile of Caenorhabditis elegans |
| title_short | Effect of milk exposure on the redox profile of Caenorhabditis elegans |
| title_sort | effect of milk exposure on the redox profile of caenorhabditis elegans |
| url | https://doi.org/10.1038/s41598-024-52254-6 |
| work_keys_str_mv | AT ingridlaisesilvestredeoliveira effectofmilkexposureontheredoxprofileofcaenorhabditiselegans AT giovannamelomartinssilva effectofmilkexposureontheredoxprofileofcaenorhabditiselegans AT cesarorlandomunozcadavid effectofmilkexposureontheredoxprofileofcaenorhabditiselegans AT daniellecavalcantisales effectofmilkexposureontheredoxprofileofcaenorhabditiselegans AT katyaanaya effectofmilkexposureontheredoxprofileofcaenorhabditiselegans AT rivadepaulaoliveira effectofmilkexposureontheredoxprofileofcaenorhabditiselegans AT adrianohenriquedonascimentorangel effectofmilkexposureontheredoxprofileofcaenorhabditiselegans |