Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection
Background Thoracic aortic aneurysm and dissection (TAAD) are devastating cardiovascular diseases with a high rate of disability and mortality. Lysine succinylation, a newly found post-translational modification, has been reported to play an important role in cardiovascular diseases. However, how su...
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PeerJ Inc.
2023-05-01
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| author | Yu Zhang Hongwei Zhang Haiyue Wang Chenhao Wang Peng Yang Chen Lu Yu Liu Zhenyuan Xu Yi Xie Jia Hu |
| author_facet | Yu Zhang Hongwei Zhang Haiyue Wang Chenhao Wang Peng Yang Chen Lu Yu Liu Zhenyuan Xu Yi Xie Jia Hu |
| author_sort | Yu Zhang |
| collection | DOAJ |
| description | Background Thoracic aortic aneurysm and dissection (TAAD) are devastating cardiovascular diseases with a high rate of disability and mortality. Lysine succinylation, a newly found post-translational modification, has been reported to play an important role in cardiovascular diseases. However, how succinylation modification influences TAAD remains obscure. Methods Ascending aortic tissues were obtained from patients with thoracic aortic aneurysm (TAA, n = 6), thoracic aortic dissection (TAD) with pre-existing aortic aneurysm (n = 6), and healthy subjects (n = 6). Global lysine succinylation level was analyzed by Western blotting. The differentially expressed proteins (DEPs) were analyzed by tandem mass tag (TMT) labeling and mass spectrometry. Succinylation-related proteins selected from the literature review and AmiGO database were set as a reference inventory for further analysis. Then, the pathological aortic sections were chosen to verify the proteomic results by Western blotting and qRT-PCR. Results The level of global lysine succinylation significantly increased in TAA and TAD patients compared with healthy subjects. Of all proteins identified by proteomic analysis, 197 common DEPs were screened both in TAA and TAD group compared with the control group, of which 93 proteins were significantly upregulated while 104 were downregulated. Among these 197 DEPs, OXCT1 overlapped with the succinylation-related proteins and was selected as the target protein involved in thoracic aortic pathogenesis. OXCT1 was further verified by Western blotting and qRT-PCR, and the results showed that OXCT1 in TAA and TAD patients was significantly lower than that in healthy donors (p < 0.001), which was consistent with the proteomic results. Conclusions OXCT1 represents novel biomarkers for lysine succinylation of TAAD and might be a therapeutic target in the future. |
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| language | English |
| last_indexed | 2024-03-09T07:56:57Z |
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| spelling | doaj.art-c110756a3db741689644b715c0bfb46c2023-12-03T00:58:50ZengPeerJ Inc.PeerJ2167-83592023-05-0111e1525810.7717/peerj.15258Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissectionYu Zhang0Hongwei Zhang1Haiyue Wang2Chenhao Wang3Peng Yang4Chen Lu5Yu Liu6Zhenyuan Xu7Yi Xie8Jia Hu9Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, ChinaBackground Thoracic aortic aneurysm and dissection (TAAD) are devastating cardiovascular diseases with a high rate of disability and mortality. Lysine succinylation, a newly found post-translational modification, has been reported to play an important role in cardiovascular diseases. However, how succinylation modification influences TAAD remains obscure. Methods Ascending aortic tissues were obtained from patients with thoracic aortic aneurysm (TAA, n = 6), thoracic aortic dissection (TAD) with pre-existing aortic aneurysm (n = 6), and healthy subjects (n = 6). Global lysine succinylation level was analyzed by Western blotting. The differentially expressed proteins (DEPs) were analyzed by tandem mass tag (TMT) labeling and mass spectrometry. Succinylation-related proteins selected from the literature review and AmiGO database were set as a reference inventory for further analysis. Then, the pathological aortic sections were chosen to verify the proteomic results by Western blotting and qRT-PCR. Results The level of global lysine succinylation significantly increased in TAA and TAD patients compared with healthy subjects. Of all proteins identified by proteomic analysis, 197 common DEPs were screened both in TAA and TAD group compared with the control group, of which 93 proteins were significantly upregulated while 104 were downregulated. Among these 197 DEPs, OXCT1 overlapped with the succinylation-related proteins and was selected as the target protein involved in thoracic aortic pathogenesis. OXCT1 was further verified by Western blotting and qRT-PCR, and the results showed that OXCT1 in TAA and TAD patients was significantly lower than that in healthy donors (p < 0.001), which was consistent with the proteomic results. Conclusions OXCT1 represents novel biomarkers for lysine succinylation of TAAD and might be a therapeutic target in the future.https://peerj.com/articles/15258.pdfTandem mass tag labellingProteomicsSuccinylationOXCT1Thoracic aortic aneurysmThoracic aortic dissection |
| spellingShingle | Yu Zhang Hongwei Zhang Haiyue Wang Chenhao Wang Peng Yang Chen Lu Yu Liu Zhenyuan Xu Yi Xie Jia Hu Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection PeerJ Tandem mass tag labelling Proteomics Succinylation OXCT1 Thoracic aortic aneurysm Thoracic aortic dissection |
| title | Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection |
| title_full | Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection |
| title_fullStr | Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection |
| title_full_unstemmed | Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection |
| title_short | Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection |
| title_sort | tandem mass tag based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection |
| topic | Tandem mass tag labelling Proteomics Succinylation OXCT1 Thoracic aortic aneurysm Thoracic aortic dissection |
| url | https://peerj.com/articles/15258.pdf |
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