Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy
Human colon carcinomas, including HCT116 cells, often exhibit high autophagic flux under nutrient deprivation or hypoxic conditions. Mitochondrial ROS (mROS) is known as a ‘molecular switch’ for regulating the autophagic pathway, which is critical for directing cancer cell survival or death. In earl...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/8/1883 |
| _version_ | 1797436566852861952 |
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| author | Eun Ji Gwak Dasol Kim Hui-Yun Hwang Ho Jeong Kwon |
| author_facet | Eun Ji Gwak Dasol Kim Hui-Yun Hwang Ho Jeong Kwon |
| author_sort | Eun Ji Gwak |
| collection | DOAJ |
| description | Human colon carcinomas, including HCT116 cells, often exhibit high autophagic flux under nutrient deprivation or hypoxic conditions. Mitochondrial ROS (mROS) is known as a ‘molecular switch’ for regulating the autophagic pathway, which is critical for directing cancer cell survival or death. In early tumorigenesis, autophagy plays important roles in maintaining cellular homeostasis and contributes to tumor growth. However, the relationships between mROS and the autophagic capacities of HCT116 cells are poorly understood. Ubiquinol cytochrome c reductase binding protein (UQCRB) has been reported as a biomarker of colorectal cancer, but its role in tumor growth has not been clarified. Here, we showed that UQCRB is overexpressed in HCT116 cells compared to CCD18co cells, a normal colon fibroblast cell line. Pharmacological inhibition of UQCRB reduced mROS levels, autophagic flux, and the growth of HCT116 tumors in a xenograft mouse model. We further investigated mutant UQCRB-overexpressing cell lines to identify functional links in UQCRB-mROS-autophagy. Notably, an increasing level of mROS caused by UQCRB overexpression released Ca<sup>2+</sup> by the activation of lysosomal transient receptor potential mucolipin 1 (TRPML1) channels. This activation induced transcription factor EB (TFEB) nuclear translocation and lysosome biogenesis, leading to autophagy flux. Collectively, our study showed that increasing levels of mROS caused by the overexpression of UQCRB in human colon carcinoma HCT116 cells could be linked to autophagy for cell survival. |
| first_indexed | 2024-03-09T11:04:29Z |
| format | Article |
| id | doaj.art-c1140839a58b41e6ad5c87a52bc783fe |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T11:04:29Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-c1140839a58b41e6ad5c87a52bc783fe2023-12-01T01:04:45ZengMDPI AGCancers2072-66942022-04-01148188310.3390/cancers14081883Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via AutophagyEun Ji Gwak0Dasol Kim1Hui-Yun Hwang2Ho Jeong Kwon3Chemical Genomics Laboratory, Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, KoreaChemical Genomics Laboratory, Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, KoreaChemical Genomics Laboratory, Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, KoreaChemical Genomics Laboratory, Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, KoreaHuman colon carcinomas, including HCT116 cells, often exhibit high autophagic flux under nutrient deprivation or hypoxic conditions. Mitochondrial ROS (mROS) is known as a ‘molecular switch’ for regulating the autophagic pathway, which is critical for directing cancer cell survival or death. In early tumorigenesis, autophagy plays important roles in maintaining cellular homeostasis and contributes to tumor growth. However, the relationships between mROS and the autophagic capacities of HCT116 cells are poorly understood. Ubiquinol cytochrome c reductase binding protein (UQCRB) has been reported as a biomarker of colorectal cancer, but its role in tumor growth has not been clarified. Here, we showed that UQCRB is overexpressed in HCT116 cells compared to CCD18co cells, a normal colon fibroblast cell line. Pharmacological inhibition of UQCRB reduced mROS levels, autophagic flux, and the growth of HCT116 tumors in a xenograft mouse model. We further investigated mutant UQCRB-overexpressing cell lines to identify functional links in UQCRB-mROS-autophagy. Notably, an increasing level of mROS caused by UQCRB overexpression released Ca<sup>2+</sup> by the activation of lysosomal transient receptor potential mucolipin 1 (TRPML1) channels. This activation induced transcription factor EB (TFEB) nuclear translocation and lysosome biogenesis, leading to autophagy flux. Collectively, our study showed that increasing levels of mROS caused by the overexpression of UQCRB in human colon carcinoma HCT116 cells could be linked to autophagy for cell survival.https://www.mdpi.com/2072-6694/14/8/1883UQCRBmROSautophagycolorectal cancerlysosome |
| spellingShingle | Eun Ji Gwak Dasol Kim Hui-Yun Hwang Ho Jeong Kwon Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy Cancers UQCRB mROS autophagy colorectal cancer lysosome |
| title | Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy |
| title_full | Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy |
| title_fullStr | Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy |
| title_full_unstemmed | Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy |
| title_short | Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy |
| title_sort | mitochondrial ros produced in human colon carcinoma associated with cell survival via autophagy |
| topic | UQCRB mROS autophagy colorectal cancer lysosome |
| url | https://www.mdpi.com/2072-6694/14/8/1883 |
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