3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways
Fluoranthene, a high-molecular-weight polycyclic aromatic hydrocarbon (PAH), is widely present in air pollutants, including fine inhalable particulate matter. 3-Bromofluoranthene (3-BrFlu), which is a brominated fluoranthene and halogenated PAH, is generated from waste combustion, metallurgical proc...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321010745 |
| _version_ | 1818572711515914240 |
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| author | Chun-Hung Su Shih-Pin Chen Li-You Chen Jiann-Jou Yang Yi-Chia Lee Shiuan-Shinn Lee Hsin-Hung Chen Yan-Yan Ng Yu-Hsiang Kuan |
| author_facet | Chun-Hung Su Shih-Pin Chen Li-You Chen Jiann-Jou Yang Yi-Chia Lee Shiuan-Shinn Lee Hsin-Hung Chen Yan-Yan Ng Yu-Hsiang Kuan |
| author_sort | Chun-Hung Su |
| collection | DOAJ |
| description | Fluoranthene, a high-molecular-weight polycyclic aromatic hydrocarbon (PAH), is widely present in air pollutants, including fine inhalable particulate matter. 3-Bromofluoranthene (3-BrFlu), which is a brominated fluoranthene and halogenated PAH, is generated from waste combustion, metallurgical processes, cement production, e-waste dismantling, and photoreaction. Vascular endothelial cells have key functions in the homeostasis and the development of the cardiovascular system. The zebrafish model has been widely employed to study cardiotoxicity and embryotoxicity. However, no evidence has indicated that 3-BrFlu induces cytotoxicity in vascular endothelial cells, or cardiotoxicity and embryotoxicity in zebrafish. In this study, 3-BrFlu induced concentration-dependent changes in embryo- and cardiotoxicity. Cytotoxicity was also induced by 3-BrFlu in a concentration-dependent manner through apoptosis and necrosis in vascular endothelial cells, SVEC4-10 cells. The activities of caspase-3, -8, and -9 were induced by 3-BrFlu via an intrinsic pathway constituting Bcl-2 downregulation, Bad upregulation, and mitochondrial dysfunction; the extrinsic pathway included the expression of death receptors, including tumour necrosis factor α and Fas receptors. These results indicated that 3-BrFlu caused cardio- and embryotoxicity in zebrafish through vascular endothelial cells cytotoxicity resulting from caspase-dependent apoptosis through intrinsic and extrinsic pathways. |
| first_indexed | 2024-12-15T00:01:16Z |
| format | Article |
| id | doaj.art-c11625813d6c4bababff5cabc9fca5b5 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-15T00:01:16Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-c11625813d6c4bababff5cabc9fca5b52022-12-21T22:42:53ZengElsevierEcotoxicology and Environmental Safety0147-65132021-12-012281129623-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathwaysChun-Hung Su0Shih-Pin Chen1Li-You Chen2Jiann-Jou Yang3Yi-Chia Lee4Shiuan-Shinn Lee5Hsin-Hung Chen6Yan-Yan Ng7Yu-Hsiang Kuan8Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan, ROC; Department of Internal Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan, ROC; Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan, ROCDepartment of Internal Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan, ROC; Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan, ROCDepartment of Anatomy, School of Medicine, College of Medicine, Chung Shan Medical University, Taichung, Taiwan, ROC; Department of Medical Education, Chung Shan Medical University Hospital, Taichung, Taiwan, ROCDepartment of BioMedical Sciences, Chung Shan Medical University, Taichung, Taiwan, ROC; Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan, ROCDepartment of Pharmacology, School of Medicine, Chung Shan Medical University, Taichung, Taiwan, ROC; Department of Pharmacy, Chung Shan Medical University Hospital, Taichung, Taiwan, ROCSchool of Public Health, Chung Shan Medical University, Taichung, Taiwan, ROCDivision of Endocrinology and Metabolism, Department of Internal Medicine, Asia University Hospital, Taichung, Taiwan, ROC; School of Medicine, Institute of Medicine and public health, Chung Shan Medical University, Taichung, Taiwan, ROC; Chung Sheng Clinic, Nantou, Taiwan, ROCDepartment of Pediatric, Chung Kang branch, Cheng Ching Hospital, Taichung City, Taiwan, ROCDepartment of Pharmacology, School of Medicine, Chung Shan Medical University, Taichung, Taiwan, ROC; Department of Pharmacy, Chung Shan Medical University Hospital, Taichung, Taiwan, ROC; Correspondence to: Department of Pharmacology, School of Medicine, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., Taichung 402, Taiwan, ROC.Fluoranthene, a high-molecular-weight polycyclic aromatic hydrocarbon (PAH), is widely present in air pollutants, including fine inhalable particulate matter. 3-Bromofluoranthene (3-BrFlu), which is a brominated fluoranthene and halogenated PAH, is generated from waste combustion, metallurgical processes, cement production, e-waste dismantling, and photoreaction. Vascular endothelial cells have key functions in the homeostasis and the development of the cardiovascular system. The zebrafish model has been widely employed to study cardiotoxicity and embryotoxicity. However, no evidence has indicated that 3-BrFlu induces cytotoxicity in vascular endothelial cells, or cardiotoxicity and embryotoxicity in zebrafish. In this study, 3-BrFlu induced concentration-dependent changes in embryo- and cardiotoxicity. Cytotoxicity was also induced by 3-BrFlu in a concentration-dependent manner through apoptosis and necrosis in vascular endothelial cells, SVEC4-10 cells. The activities of caspase-3, -8, and -9 were induced by 3-BrFlu via an intrinsic pathway constituting Bcl-2 downregulation, Bad upregulation, and mitochondrial dysfunction; the extrinsic pathway included the expression of death receptors, including tumour necrosis factor α and Fas receptors. These results indicated that 3-BrFlu caused cardio- and embryotoxicity in zebrafish through vascular endothelial cells cytotoxicity resulting from caspase-dependent apoptosis through intrinsic and extrinsic pathways.http://www.sciencedirect.com/science/article/pii/S01476513210107453-BromofluorantheneZebrafishVascular endothelial cellsCaspase-dependent apoptosis |
| spellingShingle | Chun-Hung Su Shih-Pin Chen Li-You Chen Jiann-Jou Yang Yi-Chia Lee Shiuan-Shinn Lee Hsin-Hung Chen Yan-Yan Ng Yu-Hsiang Kuan 3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways Ecotoxicology and Environmental Safety 3-Bromofluoranthene Zebrafish Vascular endothelial cells Caspase-dependent apoptosis |
| title | 3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways |
| title_full | 3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways |
| title_fullStr | 3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways |
| title_full_unstemmed | 3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways |
| title_short | 3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways |
| title_sort | 3 bromofluoranthene induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase dependent pathways |
| topic | 3-Bromofluoranthene Zebrafish Vascular endothelial cells Caspase-dependent apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651321010745 |
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