Analysis of Nuclear Dynamics in Nematode-Trapping Fungi Based on Fluorescent Protein Labeling
Nematophagous fungi constitute a category of fungi that exhibit parasitic behavior by capturing, colonizing, and poisoning nematodes, which are critical factors in controlling nematode populations in nature, and provide important research materials for biological control. <i>Arthrobotrys oligo...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/9/12/1183 |
| _version_ | 1797380431729917952 |
|---|---|
| author | Liang Zhou Zhiwei He Keqin Zhang Xin Wang |
| author_facet | Liang Zhou Zhiwei He Keqin Zhang Xin Wang |
| author_sort | Liang Zhou |
| collection | DOAJ |
| description | Nematophagous fungi constitute a category of fungi that exhibit parasitic behavior by capturing, colonizing, and poisoning nematodes, which are critical factors in controlling nematode populations in nature, and provide important research materials for biological control. <i>Arthrobotrys oligospora</i> serves as a model strain among nematophagous fungi, which begins its life as conidia, and then its hyphae produce traps to capture nematodes, completing its lifestyle switch from saprophytic to parasitic. There have been many descriptions of the morphological characteristics of <i>A. oligospora</i> lifestyle changes, but there have been no reports on the nuclear dynamics in this species. In this work, we constructed <i>A. oligospora</i> strains labeled with histone H<sub>2</sub>B–EGFP and observed the nuclear dynamics from conidia germination and hyphal extension to trap formation. We conducted real-time imaging observations on live cells of germinating and extending hyphae and found that the nucleus was located near the tip. It is interesting that the migration rate of this type of cell nucleus is very fast, and we speculate that this may be related to the morphological changes involved in the transformation to a predatory lifestyle. We suggest that alterations in nuclear shape and fixation imply the immediate disruption of the interaction with cytoskeletal mechanisms during nuclear migration. In conclusion, these findings suggest that the signal initiating nuclear migration into fungal traps is generated at the onset of nucleus entry into a trap cell. Our work provides a reference for analysis of the dynamics of nucleus distribution and a means to visualize protein localization and interactions in <i>A. oligospora</i>. |
| first_indexed | 2024-03-08T20:37:09Z |
| format | Article |
| id | doaj.art-c117720ca143447b9c22437c1d5430ca |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-08T20:37:09Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-c117720ca143447b9c22437c1d5430ca2023-12-22T14:19:25ZengMDPI AGJournal of Fungi2309-608X2023-12-01912118310.3390/jof9121183Analysis of Nuclear Dynamics in Nematode-Trapping Fungi Based on Fluorescent Protein LabelingLiang Zhou0Zhiwei He1Keqin Zhang2Xin Wang3State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650500, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650500, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650500, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650500, ChinaNematophagous fungi constitute a category of fungi that exhibit parasitic behavior by capturing, colonizing, and poisoning nematodes, which are critical factors in controlling nematode populations in nature, and provide important research materials for biological control. <i>Arthrobotrys oligospora</i> serves as a model strain among nematophagous fungi, which begins its life as conidia, and then its hyphae produce traps to capture nematodes, completing its lifestyle switch from saprophytic to parasitic. There have been many descriptions of the morphological characteristics of <i>A. oligospora</i> lifestyle changes, but there have been no reports on the nuclear dynamics in this species. In this work, we constructed <i>A. oligospora</i> strains labeled with histone H<sub>2</sub>B–EGFP and observed the nuclear dynamics from conidia germination and hyphal extension to trap formation. We conducted real-time imaging observations on live cells of germinating and extending hyphae and found that the nucleus was located near the tip. It is interesting that the migration rate of this type of cell nucleus is very fast, and we speculate that this may be related to the morphological changes involved in the transformation to a predatory lifestyle. We suggest that alterations in nuclear shape and fixation imply the immediate disruption of the interaction with cytoskeletal mechanisms during nuclear migration. In conclusion, these findings suggest that the signal initiating nuclear migration into fungal traps is generated at the onset of nucleus entry into a trap cell. Our work provides a reference for analysis of the dynamics of nucleus distribution and a means to visualize protein localization and interactions in <i>A. oligospora</i>.https://www.mdpi.com/2309-608X/9/12/1183<i>Arthrobotrys oligospora</i>histone H<sub>2</sub>BEGFPbiological control |
| spellingShingle | Liang Zhou Zhiwei He Keqin Zhang Xin Wang Analysis of Nuclear Dynamics in Nematode-Trapping Fungi Based on Fluorescent Protein Labeling Journal of Fungi <i>Arthrobotrys oligospora</i> histone H<sub>2</sub>B EGFP biological control |
| title | Analysis of Nuclear Dynamics in Nematode-Trapping Fungi Based on Fluorescent Protein Labeling |
| title_full | Analysis of Nuclear Dynamics in Nematode-Trapping Fungi Based on Fluorescent Protein Labeling |
| title_fullStr | Analysis of Nuclear Dynamics in Nematode-Trapping Fungi Based on Fluorescent Protein Labeling |
| title_full_unstemmed | Analysis of Nuclear Dynamics in Nematode-Trapping Fungi Based on Fluorescent Protein Labeling |
| title_short | Analysis of Nuclear Dynamics in Nematode-Trapping Fungi Based on Fluorescent Protein Labeling |
| title_sort | analysis of nuclear dynamics in nematode trapping fungi based on fluorescent protein labeling |
| topic | <i>Arthrobotrys oligospora</i> histone H<sub>2</sub>B EGFP biological control |
| url | https://www.mdpi.com/2309-608X/9/12/1183 |
| work_keys_str_mv | AT liangzhou analysisofnucleardynamicsinnematodetrappingfungibasedonfluorescentproteinlabeling AT zhiweihe analysisofnucleardynamicsinnematodetrappingfungibasedonfluorescentproteinlabeling AT keqinzhang analysisofnucleardynamicsinnematodetrappingfungibasedonfluorescentproteinlabeling AT xinwang analysisofnucleardynamicsinnematodetrappingfungibasedonfluorescentproteinlabeling |