Detection and genetic diversity of subgroup K avian leukosis virus in local chicken breeds in Jiangxi from 2021 to 2023
Avian leukosis virus subgroup K (ALV-K) is a new subgroup of avian leukosis virus (ALV) that was first identified in Chinese native chickens in recent years. To further understand the molecular epidemiology and evolutionary diversity of ALV-K, this study investigated the molecular epidemiology of 73...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1341201/full |
| _version_ | 1797320916794867712 |
|---|---|
| author | Fanfan Zhang Haiqin Li Cui Lin Yue Wei Weihong Zhang Yanping Wu Zhaofeng Kang |
| author_facet | Fanfan Zhang Haiqin Li Cui Lin Yue Wei Weihong Zhang Yanping Wu Zhaofeng Kang |
| author_sort | Fanfan Zhang |
| collection | DOAJ |
| description | Avian leukosis virus subgroup K (ALV-K) is a new subgroup of avian leukosis virus (ALV) that was first identified in Chinese native chickens in recent years. To further understand the molecular epidemiology and evolutionary diversity of ALV-K, this study investigated the molecular epidemiology of 73,664 chicken plasma samples collected from Jiangxi native chicken flocks. The results showed that ALV-J was the most predominant ALV subtype in Jiangxi native chickens, with a high positivity rate of 4.34%. From 2021 to 2023, there was a gradual upward trend in the proportion of positive numbers of ALV-K among ALV-positive samples, and there was a trend of outbreaks. ALV-J and ALV-K were the main co-infection patterns. Genetic evolutionary analysis based on ALV-K gp85 gene showed that the isolated ALV-K in this study were distributed in various branches of the evolutionary tree with genetic diversity. The homology results showed that the amino acid homology of the isolated ALV-K gp85 gene ranged from 33.9 to 88.1% with the reference strains of subtypes A, B, C, D, E, and J, and from 91.9 to 100% with the other ALV-K reference strains. Multiple mutations were present in the ALV-K gp85, and especially significant mutations were found in the highly variable region hr2. The results of ALV-K replication efficiency showed that the replication efficiency of ALV-K was significantly lower than that of ALV-J. These results enriched the genome sequence data of ALV-K in Chinese geoducks, and laid the foundation for further research on the pathogenesis and prevention of ALV-K. |
| first_indexed | 2024-03-08T04:49:59Z |
| format | Article |
| id | doaj.art-c118e49d56f74892bef229dc04e4973d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T04:49:59Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c118e49d56f74892bef229dc04e4973d2024-02-08T05:20:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-02-011510.3389/fmicb.2024.13412011341201Detection and genetic diversity of subgroup K avian leukosis virus in local chicken breeds in Jiangxi from 2021 to 2023Fanfan ZhangHaiqin LiCui LinYue WeiWeihong ZhangYanping WuZhaofeng KangAvian leukosis virus subgroup K (ALV-K) is a new subgroup of avian leukosis virus (ALV) that was first identified in Chinese native chickens in recent years. To further understand the molecular epidemiology and evolutionary diversity of ALV-K, this study investigated the molecular epidemiology of 73,664 chicken plasma samples collected from Jiangxi native chicken flocks. The results showed that ALV-J was the most predominant ALV subtype in Jiangxi native chickens, with a high positivity rate of 4.34%. From 2021 to 2023, there was a gradual upward trend in the proportion of positive numbers of ALV-K among ALV-positive samples, and there was a trend of outbreaks. ALV-J and ALV-K were the main co-infection patterns. Genetic evolutionary analysis based on ALV-K gp85 gene showed that the isolated ALV-K in this study were distributed in various branches of the evolutionary tree with genetic diversity. The homology results showed that the amino acid homology of the isolated ALV-K gp85 gene ranged from 33.9 to 88.1% with the reference strains of subtypes A, B, C, D, E, and J, and from 91.9 to 100% with the other ALV-K reference strains. Multiple mutations were present in the ALV-K gp85, and especially significant mutations were found in the highly variable region hr2. The results of ALV-K replication efficiency showed that the replication efficiency of ALV-K was significantly lower than that of ALV-J. These results enriched the genome sequence data of ALV-K in Chinese geoducks, and laid the foundation for further research on the pathogenesis and prevention of ALV-K.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1341201/fullavian leukosis virus subgroup Kprevalencegenetic diversitygp85 geneavian leukosis virus |
| spellingShingle | Fanfan Zhang Haiqin Li Cui Lin Yue Wei Weihong Zhang Yanping Wu Zhaofeng Kang Detection and genetic diversity of subgroup K avian leukosis virus in local chicken breeds in Jiangxi from 2021 to 2023 Frontiers in Microbiology avian leukosis virus subgroup K prevalence genetic diversity gp85 gene avian leukosis virus |
| title | Detection and genetic diversity of subgroup K avian leukosis virus in local chicken breeds in Jiangxi from 2021 to 2023 |
| title_full | Detection and genetic diversity of subgroup K avian leukosis virus in local chicken breeds in Jiangxi from 2021 to 2023 |
| title_fullStr | Detection and genetic diversity of subgroup K avian leukosis virus in local chicken breeds in Jiangxi from 2021 to 2023 |
| title_full_unstemmed | Detection and genetic diversity of subgroup K avian leukosis virus in local chicken breeds in Jiangxi from 2021 to 2023 |
| title_short | Detection and genetic diversity of subgroup K avian leukosis virus in local chicken breeds in Jiangxi from 2021 to 2023 |
| title_sort | detection and genetic diversity of subgroup k avian leukosis virus in local chicken breeds in jiangxi from 2021 to 2023 |
| topic | avian leukosis virus subgroup K prevalence genetic diversity gp85 gene avian leukosis virus |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1341201/full |
| work_keys_str_mv | AT fanfanzhang detectionandgeneticdiversityofsubgroupkavianleukosisvirusinlocalchickenbreedsinjiangxifrom2021to2023 AT haiqinli detectionandgeneticdiversityofsubgroupkavianleukosisvirusinlocalchickenbreedsinjiangxifrom2021to2023 AT cuilin detectionandgeneticdiversityofsubgroupkavianleukosisvirusinlocalchickenbreedsinjiangxifrom2021to2023 AT yuewei detectionandgeneticdiversityofsubgroupkavianleukosisvirusinlocalchickenbreedsinjiangxifrom2021to2023 AT weihongzhang detectionandgeneticdiversityofsubgroupkavianleukosisvirusinlocalchickenbreedsinjiangxifrom2021to2023 AT yanpingwu detectionandgeneticdiversityofsubgroupkavianleukosisvirusinlocalchickenbreedsinjiangxifrom2021to2023 AT zhaofengkang detectionandgeneticdiversityofsubgroupkavianleukosisvirusinlocalchickenbreedsinjiangxifrom2021to2023 |