Bioluminescence Sensing in 3D Spherical Microtissues for Multiple Bioactivity Analysis of Environmental Samples
The development of predictive <i>in vitro</i> sensing tools able to provide rapid information on the different bioactivities of a sample is of pivotal importance, not only to monitor environmental toxicants, but also to understand their mechanisms of action on diverse molecular pathways....
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/22/12/4568 |
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| author | Maria Maddalena Calabretta Denise Gregucci Tiziana Guarnieri Marina Bonini Elisa Neri Martina Zangheri Elisa Michelini |
| author_facet | Maria Maddalena Calabretta Denise Gregucci Tiziana Guarnieri Marina Bonini Elisa Neri Martina Zangheri Elisa Michelini |
| author_sort | Maria Maddalena Calabretta |
| collection | DOAJ |
| description | The development of predictive <i>in vitro</i> sensing tools able to provide rapid information on the different bioactivities of a sample is of pivotal importance, not only to monitor environmental toxicants, but also to understand their mechanisms of action on diverse molecular pathways. This mechanistic understanding is highly important for the characterization of toxicological hazards, and for the risk assessment of chemicals and environmental samples such as surface waters and effluents. Prompted by this need, we developed and optimized a straightforward bioluminescent multiplexed assay which enables the measurement of four bioactivities, selected for their relevance from a toxicological perspective, in bioluminescent microtissues. The assay was developed to monitor inflammatory, antioxidant, and toxic activity, and the presence of heavy metals, and was successfully applied to the analysis of river water samples, showing potential applicability for environmental analyses. The assay, which does not require advanced equipment, can be easily implemented in general laboratories equipped with basic cell culture facilities and a luminometer. |
| first_indexed | 2024-03-09T22:32:14Z |
| format | Article |
| id | doaj.art-c1195832376b4e4190e7ce5f28ccbe69 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T22:32:14Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-c1195832376b4e4190e7ce5f28ccbe692023-11-23T18:55:27ZengMDPI AGSensors1424-82202022-06-012212456810.3390/s22124568Bioluminescence Sensing in 3D Spherical Microtissues for Multiple Bioactivity Analysis of Environmental SamplesMaria Maddalena Calabretta0Denise Gregucci1Tiziana Guarnieri2Marina Bonini3Elisa Neri4Martina Zangheri5Elisa Michelini6Department of Chemistry “Giacomo Ciamician”, Alma Mater Studiorum—University of Bologna, Via Selmi 2, 40126 Bologna, ItalyDepartment of Chemistry “Giacomo Ciamician”, Alma Mater Studiorum—University of Bologna, Via Selmi 2, 40126 Bologna, ItalyCell Physiology Laboratory, Department of Biological, Geological and Environmental Sciences (BiGeA), Alma Mater Studiorum Università di Bologna, 40126 Bologna, ItalyARPAE Emilia-Romagna Laboratorio Multisito, Sede di Bologna, Via F Rocchi 19, 40128 Bologna, ItalyARPAE Emilia-Romagna Laboratorio Multisito, Sede di Bologna, Via F Rocchi 19, 40128 Bologna, ItalyDepartment of Chemistry “Giacomo Ciamician”, Alma Mater Studiorum—University of Bologna, Via Selmi 2, 40126 Bologna, ItalyDepartment of Chemistry “Giacomo Ciamician”, Alma Mater Studiorum—University of Bologna, Via Selmi 2, 40126 Bologna, ItalyThe development of predictive <i>in vitro</i> sensing tools able to provide rapid information on the different bioactivities of a sample is of pivotal importance, not only to monitor environmental toxicants, but also to understand their mechanisms of action on diverse molecular pathways. This mechanistic understanding is highly important for the characterization of toxicological hazards, and for the risk assessment of chemicals and environmental samples such as surface waters and effluents. Prompted by this need, we developed and optimized a straightforward bioluminescent multiplexed assay which enables the measurement of four bioactivities, selected for their relevance from a toxicological perspective, in bioluminescent microtissues. The assay was developed to monitor inflammatory, antioxidant, and toxic activity, and the presence of heavy metals, and was successfully applied to the analysis of river water samples, showing potential applicability for environmental analyses. The assay, which does not require advanced equipment, can be easily implemented in general laboratories equipped with basic cell culture facilities and a luminometer.https://www.mdpi.com/1424-8220/22/12/4568bioluminescence3D cell modelsmicrotissueanti-oxidant activityinflammatory activityheavy metals |
| spellingShingle | Maria Maddalena Calabretta Denise Gregucci Tiziana Guarnieri Marina Bonini Elisa Neri Martina Zangheri Elisa Michelini Bioluminescence Sensing in 3D Spherical Microtissues for Multiple Bioactivity Analysis of Environmental Samples Sensors bioluminescence 3D cell models microtissue anti-oxidant activity inflammatory activity heavy metals |
| title | Bioluminescence Sensing in 3D Spherical Microtissues for Multiple Bioactivity Analysis of Environmental Samples |
| title_full | Bioluminescence Sensing in 3D Spherical Microtissues for Multiple Bioactivity Analysis of Environmental Samples |
| title_fullStr | Bioluminescence Sensing in 3D Spherical Microtissues for Multiple Bioactivity Analysis of Environmental Samples |
| title_full_unstemmed | Bioluminescence Sensing in 3D Spherical Microtissues for Multiple Bioactivity Analysis of Environmental Samples |
| title_short | Bioluminescence Sensing in 3D Spherical Microtissues for Multiple Bioactivity Analysis of Environmental Samples |
| title_sort | bioluminescence sensing in 3d spherical microtissues for multiple bioactivity analysis of environmental samples |
| topic | bioluminescence 3D cell models microtissue anti-oxidant activity inflammatory activity heavy metals |
| url | https://www.mdpi.com/1424-8220/22/12/4568 |
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