Tumor-Promoting Actions of HNRNP A1 in HCC Are Associated with Cell Cycle, Mitochondrial Dynamics, and Necroptosis
Hepatocellular carcinoma (HCC) is one of the most frequent malignancies in the world. Although increasing evidence supports the role of heterogeneous ribonucleoprotein particle A1 (HNRNP A1) in tumor progression, the function of HNRNP A1 in HCC remains unclear. Here, we focused on the role of HNRNP...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/18/10209 |
| _version_ | 1797487531362615296 |
|---|---|
| author | Biao Zhao Xiaochen Lv Xiaoqi Zhao Subinuer Maimaitiaili Yuheng Zhang Ke Su Hang Yu Cheng Liu Tong Qiao |
| author_facet | Biao Zhao Xiaochen Lv Xiaoqi Zhao Subinuer Maimaitiaili Yuheng Zhang Ke Su Hang Yu Cheng Liu Tong Qiao |
| author_sort | Biao Zhao |
| collection | DOAJ |
| description | Hepatocellular carcinoma (HCC) is one of the most frequent malignancies in the world. Although increasing evidence supports the role of heterogeneous ribonucleoprotein particle A1 (HNRNP A1) in tumor progression, the function of HNRNP A1 in HCC remains unclear. Here, we focused on the role of HNRNP A1 in the development of HCC. In this study, we found HNRNP A1 participates in many aspects of HCC, such as progression and prognosis. Our results showed that HNRNP A1 is upregulated in human HCC tissues and cell lines. High expression of HNRNP A1 can promote the proliferation, migration, and invasion in HCC cells and accelerate tumor progression in mice. Moreover, we found that HNRNP A1 prevents the senescence process of HCC cells. Knocking down of HNRNP A1 promotes the expression of P16<sup>INK4</sup>, which arrests the cell cycle and then induces the senescence phenotype in HCC cells. Furthermore, we found that HNRNP A1 regulated necroptosis and mitochondrial dynamics. In summary, our study indicates that HNRNP A1 promotes the development of HCC, which suggests a potential therapeutic target for HCC. |
| first_indexed | 2024-03-09T23:49:02Z |
| format | Article |
| id | doaj.art-c11b1b79b15c43279230d2921eeff915 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:49:02Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c11b1b79b15c43279230d2921eeff9152023-11-23T16:37:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181020910.3390/ijms231810209Tumor-Promoting Actions of HNRNP A1 in HCC Are Associated with Cell Cycle, Mitochondrial Dynamics, and NecroptosisBiao Zhao0Xiaochen Lv1Xiaoqi Zhao2Subinuer Maimaitiaili3Yuheng Zhang4Ke Su5Hang Yu6Cheng Liu7Tong Qiao8Department of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, ChinaDepartment of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, ChinaDepartment of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, ChinaDepartment of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, ChinaDepartment of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, ChinaDepartment of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, ChinaDepartment of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, ChinaDepartment of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, ChinaDepartment of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, ChinaHepatocellular carcinoma (HCC) is one of the most frequent malignancies in the world. Although increasing evidence supports the role of heterogeneous ribonucleoprotein particle A1 (HNRNP A1) in tumor progression, the function of HNRNP A1 in HCC remains unclear. Here, we focused on the role of HNRNP A1 in the development of HCC. In this study, we found HNRNP A1 participates in many aspects of HCC, such as progression and prognosis. Our results showed that HNRNP A1 is upregulated in human HCC tissues and cell lines. High expression of HNRNP A1 can promote the proliferation, migration, and invasion in HCC cells and accelerate tumor progression in mice. Moreover, we found that HNRNP A1 prevents the senescence process of HCC cells. Knocking down of HNRNP A1 promotes the expression of P16<sup>INK4</sup>, which arrests the cell cycle and then induces the senescence phenotype in HCC cells. Furthermore, we found that HNRNP A1 regulated necroptosis and mitochondrial dynamics. In summary, our study indicates that HNRNP A1 promotes the development of HCC, which suggests a potential therapeutic target for HCC.https://www.mdpi.com/1422-0067/23/18/10209HNRNP A1HCCcell senescencecell cycleP16<sup>INK4</sup> |
| spellingShingle | Biao Zhao Xiaochen Lv Xiaoqi Zhao Subinuer Maimaitiaili Yuheng Zhang Ke Su Hang Yu Cheng Liu Tong Qiao Tumor-Promoting Actions of HNRNP A1 in HCC Are Associated with Cell Cycle, Mitochondrial Dynamics, and Necroptosis International Journal of Molecular Sciences HNRNP A1 HCC cell senescence cell cycle P16<sup>INK4</sup> |
| title | Tumor-Promoting Actions of HNRNP A1 in HCC Are Associated with Cell Cycle, Mitochondrial Dynamics, and Necroptosis |
| title_full | Tumor-Promoting Actions of HNRNP A1 in HCC Are Associated with Cell Cycle, Mitochondrial Dynamics, and Necroptosis |
| title_fullStr | Tumor-Promoting Actions of HNRNP A1 in HCC Are Associated with Cell Cycle, Mitochondrial Dynamics, and Necroptosis |
| title_full_unstemmed | Tumor-Promoting Actions of HNRNP A1 in HCC Are Associated with Cell Cycle, Mitochondrial Dynamics, and Necroptosis |
| title_short | Tumor-Promoting Actions of HNRNP A1 in HCC Are Associated with Cell Cycle, Mitochondrial Dynamics, and Necroptosis |
| title_sort | tumor promoting actions of hnrnp a1 in hcc are associated with cell cycle mitochondrial dynamics and necroptosis |
| topic | HNRNP A1 HCC cell senescence cell cycle P16<sup>INK4</sup> |
| url | https://www.mdpi.com/1422-0067/23/18/10209 |
| work_keys_str_mv | AT biaozhao tumorpromotingactionsofhnrnpa1inhccareassociatedwithcellcyclemitochondrialdynamicsandnecroptosis AT xiaochenlv tumorpromotingactionsofhnrnpa1inhccareassociatedwithcellcyclemitochondrialdynamicsandnecroptosis AT xiaoqizhao tumorpromotingactionsofhnrnpa1inhccareassociatedwithcellcyclemitochondrialdynamicsandnecroptosis AT subinuermaimaitiaili tumorpromotingactionsofhnrnpa1inhccareassociatedwithcellcyclemitochondrialdynamicsandnecroptosis AT yuhengzhang tumorpromotingactionsofhnrnpa1inhccareassociatedwithcellcyclemitochondrialdynamicsandnecroptosis AT kesu tumorpromotingactionsofhnrnpa1inhccareassociatedwithcellcyclemitochondrialdynamicsandnecroptosis AT hangyu tumorpromotingactionsofhnrnpa1inhccareassociatedwithcellcyclemitochondrialdynamicsandnecroptosis AT chengliu tumorpromotingactionsofhnrnpa1inhccareassociatedwithcellcyclemitochondrialdynamicsandnecroptosis AT tongqiao tumorpromotingactionsofhnrnpa1inhccareassociatedwithcellcyclemitochondrialdynamicsandnecroptosis |