Evaluation of two molecular detection platforms for gastroenteritis pathogens in treated sewage water in the Eastern province of Saudi Arabia
Abstract The ability to screen environmental water samples for gastroenteritis pathogens, particularly viruses remains challenging. Here, we investigated the presence of enteric viruses in treated sewage effluent water samples collected from a cooling tower in The Kingdom of Saudi Arabia (SA) from 2...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-25702-4 |
| _version_ | 1797980177013145600 |
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| author | Fawaz A. Al-Wohaib Ibtihaj Al-Sharif Hassan Al-Zain Donna Murad Layla Al-Harbi Maha Al-Mozaini |
| author_facet | Fawaz A. Al-Wohaib Ibtihaj Al-Sharif Hassan Al-Zain Donna Murad Layla Al-Harbi Maha Al-Mozaini |
| author_sort | Fawaz A. Al-Wohaib |
| collection | DOAJ |
| description | Abstract The ability to screen environmental water samples for gastroenteritis pathogens, particularly viruses remains challenging. Here, we investigated the presence of enteric viruses in treated sewage effluent water samples collected from a cooling tower in The Kingdom of Saudi Arabia (SA) from 2018 to 2019. Our ultimate aim was to determine the optimal handling and processing conditions for the water samples and the most sensitive detection method for the assessment of viral contamination. Sewage was collected before and after treatment at three defined zones. Samples were concentrated by ultracentrifugation and analyzed using a multiplexed bead-based assay system (Luminex technology) or multiplex PCR (QIAstat-Dx). The efficiency of these modalities to accurately detect virus contamination were subsequently compared. In total, 64 samples (16 controls and four treated samples per-control) were analyzed for 26 enteric pathogens. Of the samples, 98.7% were negative for viruses following treatment. Detection rates were higher for the multiplex PCR (QIAstat-Dx) system compared to the hybridization method, highlighting its higher sensitivity. The current water sewage treatment protocols in KSA could efficiently eradicate viral pathogens, minimizing their potential for waterborne transmission. We provide the first systematic analysis of two molecular detection methods for the assessment of gastroenteritis-associated pathogens from environmental samples in KSA. We conclude that the multiplex PCR (QIAstat-Dx) system outperforms the Luminex technology for the detection of virus pathogens in treated water samples. |
| first_indexed | 2024-04-11T05:50:15Z |
| format | Article |
| id | doaj.art-c11bd59513f749e28f160ff9a138c244 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T05:50:15Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c11bd59513f749e28f160ff9a138c2442022-12-22T04:42:05ZengNature PortfolioScientific Reports2045-23222022-12-011211710.1038/s41598-022-25702-4Evaluation of two molecular detection platforms for gastroenteritis pathogens in treated sewage water in the Eastern province of Saudi ArabiaFawaz A. Al-Wohaib0Ibtihaj Al-Sharif1Hassan Al-Zain2Donna Murad3Layla Al-Harbi4Maha Al-Mozaini5Environmental Health Unit, Workplace Environment Division, Environmental Protection, Saudi AramcoImmunocompromised Host Research Section, Department of Infection and Immunity, King Faisal Specialist Hospital and Research CentreEnvironmental Health Unit, Workplace Environment Division, Environmental Protection, Saudi AramcoImmunocompromised Host Research Section, Department of Infection and Immunity, King Faisal Specialist Hospital and Research CentreImmunocompromised Host Research Section, Department of Infection and Immunity, King Faisal Specialist Hospital and Research CentreImmunocompromised Host Research Section, Department of Infection and Immunity, King Faisal Specialist Hospital and Research CentreAbstract The ability to screen environmental water samples for gastroenteritis pathogens, particularly viruses remains challenging. Here, we investigated the presence of enteric viruses in treated sewage effluent water samples collected from a cooling tower in The Kingdom of Saudi Arabia (SA) from 2018 to 2019. Our ultimate aim was to determine the optimal handling and processing conditions for the water samples and the most sensitive detection method for the assessment of viral contamination. Sewage was collected before and after treatment at three defined zones. Samples were concentrated by ultracentrifugation and analyzed using a multiplexed bead-based assay system (Luminex technology) or multiplex PCR (QIAstat-Dx). The efficiency of these modalities to accurately detect virus contamination were subsequently compared. In total, 64 samples (16 controls and four treated samples per-control) were analyzed for 26 enteric pathogens. Of the samples, 98.7% were negative for viruses following treatment. Detection rates were higher for the multiplex PCR (QIAstat-Dx) system compared to the hybridization method, highlighting its higher sensitivity. The current water sewage treatment protocols in KSA could efficiently eradicate viral pathogens, minimizing their potential for waterborne transmission. We provide the first systematic analysis of two molecular detection methods for the assessment of gastroenteritis-associated pathogens from environmental samples in KSA. We conclude that the multiplex PCR (QIAstat-Dx) system outperforms the Luminex technology for the detection of virus pathogens in treated water samples.https://doi.org/10.1038/s41598-022-25702-4 |
| spellingShingle | Fawaz A. Al-Wohaib Ibtihaj Al-Sharif Hassan Al-Zain Donna Murad Layla Al-Harbi Maha Al-Mozaini Evaluation of two molecular detection platforms for gastroenteritis pathogens in treated sewage water in the Eastern province of Saudi Arabia Scientific Reports |
| title | Evaluation of two molecular detection platforms for gastroenteritis pathogens in treated sewage water in the Eastern province of Saudi Arabia |
| title_full | Evaluation of two molecular detection platforms for gastroenteritis pathogens in treated sewage water in the Eastern province of Saudi Arabia |
| title_fullStr | Evaluation of two molecular detection platforms for gastroenteritis pathogens in treated sewage water in the Eastern province of Saudi Arabia |
| title_full_unstemmed | Evaluation of two molecular detection platforms for gastroenteritis pathogens in treated sewage water in the Eastern province of Saudi Arabia |
| title_short | Evaluation of two molecular detection platforms for gastroenteritis pathogens in treated sewage water in the Eastern province of Saudi Arabia |
| title_sort | evaluation of two molecular detection platforms for gastroenteritis pathogens in treated sewage water in the eastern province of saudi arabia |
| url | https://doi.org/10.1038/s41598-022-25702-4 |
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