Swimming Attenuates Muscle Wasting and Mediates Multiple Signaling Pathways and Metabolites in CT-26 Bearing Mice
Objectives: To investigate the effects of swimming on cancer induced muscle wasting and explore its underlying mechanism in CT-26 bearing mice.Methods: BALB/c mice (n = 16) injected with CT-26 cells were divided into two groups, including Tumor group (n = 8) and Swimming group (n = 8). Another 8 un-...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.812681/full |
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| author | Jiapeng Li Jiapeng Li Qiurong Xie Qiurong Xie Liya Liu Liya Liu Ying Cheng Ying Cheng Yuying Han Yuying Han Xiaoping Chen Xiaoping Chen Jia Lin Zuanfang Li Zuanfang Li Huixin Liu Huixin Liu Xiuli Zhang Xiuli Zhang Haichun Chen Haichun Chen Jun Peng Jun Peng Aling Shen Aling Shen |
| author_facet | Jiapeng Li Jiapeng Li Qiurong Xie Qiurong Xie Liya Liu Liya Liu Ying Cheng Ying Cheng Yuying Han Yuying Han Xiaoping Chen Xiaoping Chen Jia Lin Zuanfang Li Zuanfang Li Huixin Liu Huixin Liu Xiuli Zhang Xiuli Zhang Haichun Chen Haichun Chen Jun Peng Jun Peng Aling Shen Aling Shen |
| author_sort | Jiapeng Li |
| collection | DOAJ |
| description | Objectives: To investigate the effects of swimming on cancer induced muscle wasting and explore its underlying mechanism in CT-26 bearing mice.Methods: BALB/c mice (n = 16) injected with CT-26 cells were divided into two groups, including Tumor group (n = 8) and Swimming group (n = 8). Another 8 un-injected mice were set as Control group. Mice in Swimming group were subjected to physical training for swimming twice per day for 30 min intervals and 6 days per week for a total of 4 weeks. The tumor volume was monitored every 3 days and tumor weight was measured at the end of experiment. The changes of muscle function, pathological and cell apoptosis of quadriceps muscles were further assessed, and its underlying mechanisms were further explored using multiple biological technologies.Results: Swimming obviously alleviated tumor volume and weight in CT-26 bearing mice. Moreover, swimming attenuated the decrease of muscle tension, autonomic activities, and increase of muscle atrophy, pathological ultrastructure, as well as cell apoptosis of quadriceps muscles in CT-26 bearing mice. Furthermore, swimming significantly down-regulated the protein expression of NF-κB, p-NF-κB, TNF-α, IL-1β, IL-6 and Bax, while up-regulated the expression of Bcl-2. Further differential expressed metabolites (DEMs) analysis identified a total of 76 (in anion mode) and 330 (in cationic mode) DEMs in quadriceps muscles of CT-26 bearing mice after swimming, including taurochenodeoxycholic acid, taurocholic acid, ascorbic acid and eicosapentaenoic acid.Conclusion: Swimming attenuates tumor growth and muscle wasting, and by suppressing the activation of NF-κB signaling pathway mediated inflammation, reducing the level of Bax medicated cell apoptosis, as well as modulating multiple metabolites might be the importantly underlying mechanisms. |
| first_indexed | 2024-04-14T04:14:35Z |
| format | Article |
| id | doaj.art-c11f326c4e7849258524b497e8121499 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-14T04:14:35Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-c11f326c4e7849258524b497e81214992022-12-22T02:12:58ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-01-01810.3389/fmolb.2021.812681812681Swimming Attenuates Muscle Wasting and Mediates Multiple Signaling Pathways and Metabolites in CT-26 Bearing MiceJiapeng Li0Jiapeng Li1Qiurong Xie2Qiurong Xie3Liya Liu4Liya Liu5Ying Cheng6Ying Cheng7Yuying Han8Yuying Han9Xiaoping Chen10Xiaoping Chen11Jia Lin12Zuanfang Li13Zuanfang Li14Huixin Liu15Huixin Liu16Xiuli Zhang17Xiuli Zhang18Haichun Chen19Haichun Chen20Jun Peng21Jun Peng22Aling Shen23Aling Shen24The Department of Physical Education, Fujian University of Traditional Chinese Medicine, Fuzhou, ChinaSchool of Physical Education and Sport Science, Fujian Normal University, Fuzhou, ChinaAcademy of Integrative Medicine, Fuzhou, ChinaFujian Key Laboratory of Integrative Medicine in Geriatrics, Fuzhou, ChinaAcademy of Integrative Medicine, Fuzhou, ChinaFujian Key Laboratory of Integrative Medicine in Geriatrics, Fuzhou, ChinaAcademy of Integrative Medicine, Fuzhou, ChinaFujian Key Laboratory of Integrative Medicine in Geriatrics, Fuzhou, ChinaAcademy of Integrative Medicine, Fuzhou, ChinaFujian Key Laboratory of Integrative Medicine in Geriatrics, Fuzhou, ChinaAcademy of Integrative Medicine, Fuzhou, ChinaFujian Key Laboratory of Integrative Medicine in Geriatrics, Fuzhou, ChinaRehabilitation Industry Institute, Fujian University of Traditional Chinese Medicine, Fuzhou, ChinaAcademy of Integrative Medicine, Fuzhou, ChinaFujian Key Laboratory of Integrative Medicine in Geriatrics, Fuzhou, ChinaAcademy of Integrative Medicine, Fuzhou, ChinaFujian Key Laboratory of Integrative Medicine in Geriatrics, Fuzhou, ChinaAcademy of Integrative Medicine, Fuzhou, ChinaFujian Key Laboratory of Integrative Medicine in Geriatrics, Fuzhou, ChinaSchool of Physical Education and Sport Science, Fujian Normal University, Fuzhou, ChinaProvincial University Key Laboratory of Sport and Health Science, School of Physical Education and Sport Sciences, Fujian Normal University, Fuzhou, ChinaAcademy of Integrative Medicine, Fuzhou, ChinaFujian Key Laboratory of Integrative Medicine in Geriatrics, Fuzhou, ChinaAcademy of Integrative Medicine, Fuzhou, ChinaFujian Key Laboratory of Integrative Medicine in Geriatrics, Fuzhou, ChinaObjectives: To investigate the effects of swimming on cancer induced muscle wasting and explore its underlying mechanism in CT-26 bearing mice.Methods: BALB/c mice (n = 16) injected with CT-26 cells were divided into two groups, including Tumor group (n = 8) and Swimming group (n = 8). Another 8 un-injected mice were set as Control group. Mice in Swimming group were subjected to physical training for swimming twice per day for 30 min intervals and 6 days per week for a total of 4 weeks. The tumor volume was monitored every 3 days and tumor weight was measured at the end of experiment. The changes of muscle function, pathological and cell apoptosis of quadriceps muscles were further assessed, and its underlying mechanisms were further explored using multiple biological technologies.Results: Swimming obviously alleviated tumor volume and weight in CT-26 bearing mice. Moreover, swimming attenuated the decrease of muscle tension, autonomic activities, and increase of muscle atrophy, pathological ultrastructure, as well as cell apoptosis of quadriceps muscles in CT-26 bearing mice. Furthermore, swimming significantly down-regulated the protein expression of NF-κB, p-NF-κB, TNF-α, IL-1β, IL-6 and Bax, while up-regulated the expression of Bcl-2. Further differential expressed metabolites (DEMs) analysis identified a total of 76 (in anion mode) and 330 (in cationic mode) DEMs in quadriceps muscles of CT-26 bearing mice after swimming, including taurochenodeoxycholic acid, taurocholic acid, ascorbic acid and eicosapentaenoic acid.Conclusion: Swimming attenuates tumor growth and muscle wasting, and by suppressing the activation of NF-κB signaling pathway mediated inflammation, reducing the level of Bax medicated cell apoptosis, as well as modulating multiple metabolites might be the importantly underlying mechanisms.https://www.frontiersin.org/articles/10.3389/fmolb.2021.812681/fullswimmingcolorectal cancermuscle wastingNF-κBmetabolite |
| spellingShingle | Jiapeng Li Jiapeng Li Qiurong Xie Qiurong Xie Liya Liu Liya Liu Ying Cheng Ying Cheng Yuying Han Yuying Han Xiaoping Chen Xiaoping Chen Jia Lin Zuanfang Li Zuanfang Li Huixin Liu Huixin Liu Xiuli Zhang Xiuli Zhang Haichun Chen Haichun Chen Jun Peng Jun Peng Aling Shen Aling Shen Swimming Attenuates Muscle Wasting and Mediates Multiple Signaling Pathways and Metabolites in CT-26 Bearing Mice Frontiers in Molecular Biosciences swimming colorectal cancer muscle wasting NF-κB metabolite |
| title | Swimming Attenuates Muscle Wasting and Mediates Multiple Signaling Pathways and Metabolites in CT-26 Bearing Mice |
| title_full | Swimming Attenuates Muscle Wasting and Mediates Multiple Signaling Pathways and Metabolites in CT-26 Bearing Mice |
| title_fullStr | Swimming Attenuates Muscle Wasting and Mediates Multiple Signaling Pathways and Metabolites in CT-26 Bearing Mice |
| title_full_unstemmed | Swimming Attenuates Muscle Wasting and Mediates Multiple Signaling Pathways and Metabolites in CT-26 Bearing Mice |
| title_short | Swimming Attenuates Muscle Wasting and Mediates Multiple Signaling Pathways and Metabolites in CT-26 Bearing Mice |
| title_sort | swimming attenuates muscle wasting and mediates multiple signaling pathways and metabolites in ct 26 bearing mice |
| topic | swimming colorectal cancer muscle wasting NF-κB metabolite |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2021.812681/full |
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