Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training
Background We sought to examine how 12 weeks of resistance exercise training (RET) affected skeletal muscle myofibrillar and sarcoplasmic protein levels along with markers of mitochondrial physiology in high versus low anabolic responders. Methods Untrained college-aged males were classified as anab...
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PeerJ Inc.
2018-07-01
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| author | Michael D. Roberts Matthew A. Romero Christopher B. Mobley Petey W. Mumford Paul A. Roberson Cody T. Haun Christopher G. Vann Shelby C. Osburn Hudson H. Holmes Rory A. Greer Christopher M. Lockwood Hailey A. Parry Andreas N. Kavazis |
| author_facet | Michael D. Roberts Matthew A. Romero Christopher B. Mobley Petey W. Mumford Paul A. Roberson Cody T. Haun Christopher G. Vann Shelby C. Osburn Hudson H. Holmes Rory A. Greer Christopher M. Lockwood Hailey A. Parry Andreas N. Kavazis |
| author_sort | Michael D. Roberts |
| collection | DOAJ |
| description | Background We sought to examine how 12 weeks of resistance exercise training (RET) affected skeletal muscle myofibrillar and sarcoplasmic protein levels along with markers of mitochondrial physiology in high versus low anabolic responders. Methods Untrained college-aged males were classified as anabolic responders in the top 25th percentile (high-response cluster (HI); n = 13, dual x-ray absorptiometry total body muscle mass change (Δ) = +3.1 ± 0.3 kg, Δ vastus lateralis (VL) thickness = +0.59 ± 0.05 cm, Δ muscle fiber cross sectional area = +1,426 ± 253 μm2) and bottom 25th percentile (low-response cluster (LO); n = 12, +1.1 ± 0.2 kg, +0.24 ± 0.07 cm, +5 ± 209 μm2; p < 0.001 for all Δ scores compared to HI). VL muscle prior to (PRE) and following RET (POST) was assayed for myofibrillar and sarcoplasmic protein concentrations, myosin and actin protein content, and markers of mitochondrial volume. Proteins related to myofibril formation, as well as whole lysate PGC1-α protein levels were assessed. Results Main effects of cluster (HI > LO, p = 0.018, Cohen’s d = 0.737) and time (PRE > POST, p = 0.037, Cohen’s d = −0.589) were observed for citrate synthase activity, although no significant interaction existed (LO PRE = 1.35 ± 0.07 mM/min/mg protein, LO POST = 1.12 ± 0.06, HI PRE = 1.53 ± 0.11, HI POST = 1.39 ± 0.10). POST myofibrillar myozenin-1 protein levels were up-regulated in the LO cluster (LO PRE = 0.96 ± 0.13 relative expression units, LO POST = 1.25 ± 0.16, HI PRE = 1.00 ± 0.11, HI POST = 0.85 ± 0.12; within-group LO increase p = 0.025, Cohen’s d = 0.691). No interactions or main effects existed for other assayed markers. Discussion Our data suggest myofibrillar or sarcoplasmic protein concentrations do not differ between HI versus LO anabolic responders prior to or following a 12-week RET program. Greater mitochondrial volume in HI responders may have facilitated greater anabolism, and myofibril myozenin-1 protein levels may represent a biomarker that differentiates anabolic responses to RET. However, mechanistic research validating these hypotheses is needed. |
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| spelling | doaj.art-c11ffc4531c2499e95b55109045a6fb62023-12-02T21:50:25ZengPeerJ Inc.PeerJ2167-83592018-07-016e533810.7717/peerj.5338Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance trainingMichael D. Roberts0Matthew A. Romero1Christopher B. Mobley2Petey W. Mumford3Paul A. Roberson4Cody T. Haun5Christopher G. Vann6Shelby C. Osburn7Hudson H. Holmes8Rory A. Greer9Christopher M. Lockwood10Hailey A. Parry11Andreas N. Kavazis12School of Kinesiology, Auburn University, Auburn, AL, USASchool of Kinesiology, Auburn University, Auburn, AL, USASchool of Kinesiology, Auburn University, Auburn, AL, USASchool of Kinesiology, Auburn University, Auburn, AL, USASchool of Kinesiology, Auburn University, Auburn, AL, USASchool of Kinesiology, Auburn University, Auburn, AL, USASchool of Kinesiology, Auburn University, Auburn, AL, USASchool of Kinesiology, Auburn University, Auburn, AL, USASchool of Kinesiology, Auburn University, Auburn, AL, USASchool of Kinesiology, Auburn University, Auburn, AL, USALockwood, LLC, Draper, UT, USASchool of Kinesiology, Auburn University, Auburn, AL, USASchool of Kinesiology, Auburn University, Auburn, AL, USABackground We sought to examine how 12 weeks of resistance exercise training (RET) affected skeletal muscle myofibrillar and sarcoplasmic protein levels along with markers of mitochondrial physiology in high versus low anabolic responders. Methods Untrained college-aged males were classified as anabolic responders in the top 25th percentile (high-response cluster (HI); n = 13, dual x-ray absorptiometry total body muscle mass change (Δ) = +3.1 ± 0.3 kg, Δ vastus lateralis (VL) thickness = +0.59 ± 0.05 cm, Δ muscle fiber cross sectional area = +1,426 ± 253 μm2) and bottom 25th percentile (low-response cluster (LO); n = 12, +1.1 ± 0.2 kg, +0.24 ± 0.07 cm, +5 ± 209 μm2; p < 0.001 for all Δ scores compared to HI). VL muscle prior to (PRE) and following RET (POST) was assayed for myofibrillar and sarcoplasmic protein concentrations, myosin and actin protein content, and markers of mitochondrial volume. Proteins related to myofibril formation, as well as whole lysate PGC1-α protein levels were assessed. Results Main effects of cluster (HI > LO, p = 0.018, Cohen’s d = 0.737) and time (PRE > POST, p = 0.037, Cohen’s d = −0.589) were observed for citrate synthase activity, although no significant interaction existed (LO PRE = 1.35 ± 0.07 mM/min/mg protein, LO POST = 1.12 ± 0.06, HI PRE = 1.53 ± 0.11, HI POST = 1.39 ± 0.10). POST myofibrillar myozenin-1 protein levels were up-regulated in the LO cluster (LO PRE = 0.96 ± 0.13 relative expression units, LO POST = 1.25 ± 0.16, HI PRE = 1.00 ± 0.11, HI POST = 0.85 ± 0.12; within-group LO increase p = 0.025, Cohen’s d = 0.691). No interactions or main effects existed for other assayed markers. Discussion Our data suggest myofibrillar or sarcoplasmic protein concentrations do not differ between HI versus LO anabolic responders prior to or following a 12-week RET program. Greater mitochondrial volume in HI responders may have facilitated greater anabolism, and myofibril myozenin-1 protein levels may represent a biomarker that differentiates anabolic responses to RET. However, mechanistic research validating these hypotheses is needed.https://peerj.com/articles/5338.pdfMYOZ1Muscle hypertrophyCitrate synthase |
| spellingShingle | Michael D. Roberts Matthew A. Romero Christopher B. Mobley Petey W. Mumford Paul A. Roberson Cody T. Haun Christopher G. Vann Shelby C. Osburn Hudson H. Holmes Rory A. Greer Christopher M. Lockwood Hailey A. Parry Andreas N. Kavazis Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training PeerJ MYOZ1 Muscle hypertrophy Citrate synthase |
| title | Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training |
| title_full | Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training |
| title_fullStr | Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training |
| title_full_unstemmed | Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training |
| title_short | Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training |
| title_sort | skeletal muscle mitochondrial volume and myozenin 1 protein differences exist between high versus low anabolic responders to resistance training |
| topic | MYOZ1 Muscle hypertrophy Citrate synthase |
| url | https://peerj.com/articles/5338.pdf |
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