A Transmissible RNA Pathway in Honey Bees
Summary: Systemic RNAi, initiated by double-stranded RNA (dsRNA) ingestion, has been reported in diverse invertebrates, including honey bees, demonstrating environmental RNA uptake that undermines homologous gene expression. However, the question why any organism would take up RNA from the environme...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-05-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124719305534 |
| _version_ | 1819261991312687104 |
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| author | Eyal Maori Yael Garbian Vered Kunik Rita Mozes-Koch Osnat Malka Haim Kalev Niv Sabath Ilan Sela Sharoni Shafir |
| author_facet | Eyal Maori Yael Garbian Vered Kunik Rita Mozes-Koch Osnat Malka Haim Kalev Niv Sabath Ilan Sela Sharoni Shafir |
| author_sort | Eyal Maori |
| collection | DOAJ |
| description | Summary: Systemic RNAi, initiated by double-stranded RNA (dsRNA) ingestion, has been reported in diverse invertebrates, including honey bees, demonstrating environmental RNA uptake that undermines homologous gene expression. However, the question why any organism would take up RNA from the environment has remained largely unanswered. Here, we report on horizontal RNA flow among honey bees mediated by secretion and ingestion of worker and royal jelly diets. We demonstrate that transmission of jelly-secreted dsRNA to larvae is biologically active and triggers gene knockdown that lasts into adulthood. Worker and royal jellies harbor differential naturally occurring RNA populations. Jelly RNAs corresponded to honey bee protein-coding genes, transposable elements, and non-coding RNA, as well as bacteria, fungi, and viruses. These results reveal an inherent property of honey bees to share RNA among individuals and generations. Our findings suggest a transmissible RNA pathway, playing a role in social immunity and signaling between members of the hive. : RNA mobility among cells has been documented in plants and animals. Maori et al. show that RNA spreads further in honey bees and is horizontally transferred between individuals and across generations. Their findings demonstrate a transmissible RNA pathway with potential roles in social immunity and epigenetic communication between honey bees. Keywords: Honey bees, environmental RNA, extracellular RNA, transmissible RNA, RNA transmission, royal jelly, RNAi, eRNA, viruses, RNP |
| first_indexed | 2024-12-23T19:50:35Z |
| format | Article |
| id | doaj.art-c120cdb4a4484881addb5bcfbcde042b |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-23T19:50:35Z |
| publishDate | 2019-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-c120cdb4a4484881addb5bcfbcde042b2022-12-21T17:33:23ZengElsevierCell Reports2211-12472019-05-0127719491959.e6A Transmissible RNA Pathway in Honey BeesEyal Maori0Yael Garbian1Vered Kunik2Rita Mozes-Koch3Osnat Malka4Haim Kalev5Niv Sabath6Ilan Sela7Sharoni Shafir8Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK; Corresponding authorThe Hebrew University of Jerusalem, The Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot 7610001, IsraelBioinformatics Consulting, 12 Hailanot Street, Gat-Rimon 4992000, IsraelThe Hebrew University of Jerusalem, The Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot 7610001, IsraelThe Hebrew University of Jerusalem, The Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot 7610001, IsraelThe Hebrew University of Jerusalem, The Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot 7610001, IsraelDepartment of Biochemistry, Rappaport Faculty of Medicine, Technion–Israel Institute of Technology, Haifa 31096, IsraelThe Hebrew University of Jerusalem, The Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot 7610001, IsraelThe Hebrew University of Jerusalem, The Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot 7610001, IsraelSummary: Systemic RNAi, initiated by double-stranded RNA (dsRNA) ingestion, has been reported in diverse invertebrates, including honey bees, demonstrating environmental RNA uptake that undermines homologous gene expression. However, the question why any organism would take up RNA from the environment has remained largely unanswered. Here, we report on horizontal RNA flow among honey bees mediated by secretion and ingestion of worker and royal jelly diets. We demonstrate that transmission of jelly-secreted dsRNA to larvae is biologically active and triggers gene knockdown that lasts into adulthood. Worker and royal jellies harbor differential naturally occurring RNA populations. Jelly RNAs corresponded to honey bee protein-coding genes, transposable elements, and non-coding RNA, as well as bacteria, fungi, and viruses. These results reveal an inherent property of honey bees to share RNA among individuals and generations. Our findings suggest a transmissible RNA pathway, playing a role in social immunity and signaling between members of the hive. : RNA mobility among cells has been documented in plants and animals. Maori et al. show that RNA spreads further in honey bees and is horizontally transferred between individuals and across generations. Their findings demonstrate a transmissible RNA pathway with potential roles in social immunity and epigenetic communication between honey bees. Keywords: Honey bees, environmental RNA, extracellular RNA, transmissible RNA, RNA transmission, royal jelly, RNAi, eRNA, viruses, RNPhttp://www.sciencedirect.com/science/article/pii/S2211124719305534 |
| spellingShingle | Eyal Maori Yael Garbian Vered Kunik Rita Mozes-Koch Osnat Malka Haim Kalev Niv Sabath Ilan Sela Sharoni Shafir A Transmissible RNA Pathway in Honey Bees Cell Reports |
| title | A Transmissible RNA Pathway in Honey Bees |
| title_full | A Transmissible RNA Pathway in Honey Bees |
| title_fullStr | A Transmissible RNA Pathway in Honey Bees |
| title_full_unstemmed | A Transmissible RNA Pathway in Honey Bees |
| title_short | A Transmissible RNA Pathway in Honey Bees |
| title_sort | transmissible rna pathway in honey bees |
| url | http://www.sciencedirect.com/science/article/pii/S2211124719305534 |
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