Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas
Age-related macular degeneration (AMD) is one of the leading causes of visual loss in western countries, it has no cure, and its incidence will grow in the future, for the overall population aging. Albino rats with retinal degeneration induced by exposure to high-intensity light (light-damage, LD) h...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/5/678 |
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| author | Stefano Di Marco Serena Riccitelli Mattia Di Paolo Emilio Campos Marina Buzzi Silvia Bisti Piera Versura |
| author_facet | Stefano Di Marco Serena Riccitelli Mattia Di Paolo Emilio Campos Marina Buzzi Silvia Bisti Piera Versura |
| author_sort | Stefano Di Marco |
| collection | DOAJ |
| description | Age-related macular degeneration (AMD) is one of the leading causes of visual loss in western countries, it has no cure, and its incidence will grow in the future, for the overall population aging. Albino rats with retinal degeneration induced by exposure to high-intensity light (light-damage, LD) have been extensively used as a model of AMD to test neuroprotective agents. Among them, trophic factors (NGF and BDNF) have been shown to play a significant role in photoreceptors’ survival. Interestingly, cord blood serum (CBS) is an extract full of chemokines and trophic factors; we, therefore, hypothesized that CBS could be an excellent candidate for neuroprotection. Here, we investigate whether CBS-based eye drops might mitigate the effects of light-induced retinal degeneration in albino rats. CBS treatment significantly preserved flash-electroretinogram (f-ERG) response after LD and reduced the “hot-spot” extension. Besides, CBS-treated animals better preserved the morphology of the outer nuclear layer, together with a reduction in microglia migration and activation. Interestingly, the treatment did not modulate reactive gliosis and activation of the self-protective mechanism (FGF2). In conclusion, our results suggest that CBS-based eye drops might be successfully used to mitigate retinal neurodegenerative processes such as AMD. |
| first_indexed | 2024-03-10T20:10:48Z |
| format | Article |
| id | doaj.art-c1249ff3e9244b8aa8582762c65ca42e |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T20:10:48Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-c1249ff3e9244b8aa8582762c65ca42e2023-11-19T22:56:24ZengMDPI AGBiomolecules2218-273X2020-04-0110567810.3390/biom10050678Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat RetinasStefano Di Marco0Serena Riccitelli1Mattia Di Paolo2Emilio Campos3Marina Buzzi4Silvia Bisti5Piera Versura6Department of Applied Clinical Science and Biotechnology, University of L’Aquila, Via Vetoio, Coppito II, 67100 L’Aquila, ItalyDepartment of Applied Clinical Science and Biotechnology, University of L’Aquila, Via Vetoio, Coppito II, 67100 L’Aquila, ItalyDepartment of Applied Clinical Science and Biotechnology, University of L’Aquila, Via Vetoio, Coppito II, 67100 L’Aquila, ItalyOphthalmology Unit, University of Bologna and S. Orsola-Malpighi Teaching Hospital, 40138 Bologna, ItalyEmilia Romagna, Cord Blood Bank-Transfusion Service, S. Orsola-Malpighi Teaching Hospital, 40138 Bologna, ItalyDepartment of Applied Clinical Science and Biotechnology, University of L’Aquila, Via Vetoio, Coppito II, 67100 L’Aquila, ItalyOphthalmology Unit, University of Bologna and S. Orsola-Malpighi Teaching Hospital, 40138 Bologna, ItalyAge-related macular degeneration (AMD) is one of the leading causes of visual loss in western countries, it has no cure, and its incidence will grow in the future, for the overall population aging. Albino rats with retinal degeneration induced by exposure to high-intensity light (light-damage, LD) have been extensively used as a model of AMD to test neuroprotective agents. Among them, trophic factors (NGF and BDNF) have been shown to play a significant role in photoreceptors’ survival. Interestingly, cord blood serum (CBS) is an extract full of chemokines and trophic factors; we, therefore, hypothesized that CBS could be an excellent candidate for neuroprotection. Here, we investigate whether CBS-based eye drops might mitigate the effects of light-induced retinal degeneration in albino rats. CBS treatment significantly preserved flash-electroretinogram (f-ERG) response after LD and reduced the “hot-spot” extension. Besides, CBS-treated animals better preserved the morphology of the outer nuclear layer, together with a reduction in microglia migration and activation. Interestingly, the treatment did not modulate reactive gliosis and activation of the self-protective mechanism (FGF2). In conclusion, our results suggest that CBS-based eye drops might be successfully used to mitigate retinal neurodegenerative processes such as AMD.https://www.mdpi.com/2218-273X/10/5/678photoreceptor neurodegenerationretinatrophic factorcord blood serum |
| spellingShingle | Stefano Di Marco Serena Riccitelli Mattia Di Paolo Emilio Campos Marina Buzzi Silvia Bisti Piera Versura Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas Biomolecules photoreceptor neurodegeneration retina trophic factor cord blood serum |
| title | Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas |
| title_full | Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas |
| title_fullStr | Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas |
| title_full_unstemmed | Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas |
| title_short | Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas |
| title_sort | cord blood serum cbs based eye drops modulate light induced neurodegeneration in albino rat retinas |
| topic | photoreceptor neurodegeneration retina trophic factor cord blood serum |
| url | https://www.mdpi.com/2218-273X/10/5/678 |
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