Spectroscopic analysis and cytotoxic activity of quas-sinoid isolated from the seeds of Brucea javanicaon Hela cell
The quassinoids from Brucea javanica exhibit biological activities as antimalarial, antitumor, antiviral and antiamoebic activities. These quassinoids having a promising antitumor activity.This research was aimed to isolated quassinoid (bruceine A, MW 522 g/mol) compound...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2011-04-01
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| Series: | Indonesian Journal of Pharmacy |
| Subjects: | |
| Online Access: | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/604/479 |
| _version_ | 1819119031650615296 |
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| author | Sonlimar Mangunsong Dwiprahasto I Mustofa Jumina |
| author_facet | Sonlimar Mangunsong Dwiprahasto I Mustofa Jumina |
| author_sort | Sonlimar Mangunsong |
| collection | DOAJ |
| description | The quassinoids from Brucea javanica exhibit biological activities as antimalarial, antitumor, antiviral and antiamoebic activities. These quassinoids having a promising antitumor activity.This research was aimed to isolated quassinoid (bruceine A, MW 522 g/mol) compound from B. javanica and to investigate the cytotoxicity of bruceine A under invitro conditions in Hela cells. The cytotoxicity assays using MTT assay. The bruceine showed a good cytotoxic activity in Hela cells at 24 hours with doxorubicine as a positive control. Structural elucidation of bruceine is done by using UV and FT-IR spectra, NMR spectra recorded on Tesla av600, (400 MHz) 1HNMR in pyridine (D5), some in Gyro (300 MHz) 1HNMR in acetone-(d6), DMSO-(d6) and CdCl3 ,13CNMR, 75 MHz, mass spectra on biospectrometry LC-MS. Analytical HPLC retention time,tRwas 4.617 minutes (Met/H20) 60:40. Its Molecular formula was determined to be ( C26 H34 O11) by the [M+H]+ ion peak at m/z 523 and m/z 1045 confirmed by High Resolution mass spectra Orbitrap (HRESIMS). From the results demonstrated that could be bruceine A compound refers to our spectral data. Its has activity in IC50 value 191,5 µM on Hela Cells under invitro conditions. |
| first_indexed | 2024-12-22T05:58:18Z |
| format | Article |
| id | doaj.art-c125a39d657e43bebd71906862d3af3c |
| institution | Directory Open Access Journal |
| issn | 2338-9427 2338-9486 |
| language | English |
| last_indexed | 2024-12-22T05:58:18Z |
| publishDate | 2011-04-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Pharmacy |
| spelling | doaj.art-c125a39d657e43bebd71906862d3af3c2022-12-21T18:36:38ZengUniversitas Gadjah MadaIndonesian Journal of Pharmacy2338-94272338-94862011-04-01222137143http://dx.doi.org/10.14499/indonesianjpharm0iss0pp137-143Spectroscopic analysis and cytotoxic activity of quas-sinoid isolated from the seeds of Brucea javanicaon Hela cellSonlimar Mangunsong0Dwiprahasto I1Mustofa2Jumina3Faculty of Medicine Universitas Gadjah MadaFaculty of Medicine Universitas Gadjah MadaFaculty of Medicine Universitas Gadjah MadaFaculty of Mathematics and Natural Sciences, Department of Chemistry Universitas Gadjah MadaThe quassinoids from Brucea javanica exhibit biological activities as antimalarial, antitumor, antiviral and antiamoebic activities. These quassinoids having a promising antitumor activity.This research was aimed to isolated quassinoid (bruceine A, MW 522 g/mol) compound from B. javanica and to investigate the cytotoxicity of bruceine A under invitro conditions in Hela cells. The cytotoxicity assays using MTT assay. The bruceine showed a good cytotoxic activity in Hela cells at 24 hours with doxorubicine as a positive control. Structural elucidation of bruceine is done by using UV and FT-IR spectra, NMR spectra recorded on Tesla av600, (400 MHz) 1HNMR in pyridine (D5), some in Gyro (300 MHz) 1HNMR in acetone-(d6), DMSO-(d6) and CdCl3 ,13CNMR, 75 MHz, mass spectra on biospectrometry LC-MS. Analytical HPLC retention time,tRwas 4.617 minutes (Met/H20) 60:40. Its Molecular formula was determined to be ( C26 H34 O11) by the [M+H]+ ion peak at m/z 523 and m/z 1045 confirmed by High Resolution mass spectra Orbitrap (HRESIMS). From the results demonstrated that could be bruceine A compound refers to our spectral data. Its has activity in IC50 value 191,5 µM on Hela Cells under invitro conditions.http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/604/479QuassinoidB. javanicaBrusein ASpectroscopicAnticancer |
| spellingShingle | Sonlimar Mangunsong Dwiprahasto I Mustofa Jumina Spectroscopic analysis and cytotoxic activity of quas-sinoid isolated from the seeds of Brucea javanicaon Hela cell Indonesian Journal of Pharmacy Quassinoid B. javanica Brusein A Spectroscopic Anticancer |
| title | Spectroscopic analysis and cytotoxic activity of quas-sinoid isolated from the seeds of Brucea javanicaon Hela cell |
| title_full | Spectroscopic analysis and cytotoxic activity of quas-sinoid isolated from the seeds of Brucea javanicaon Hela cell |
| title_fullStr | Spectroscopic analysis and cytotoxic activity of quas-sinoid isolated from the seeds of Brucea javanicaon Hela cell |
| title_full_unstemmed | Spectroscopic analysis and cytotoxic activity of quas-sinoid isolated from the seeds of Brucea javanicaon Hela cell |
| title_short | Spectroscopic analysis and cytotoxic activity of quas-sinoid isolated from the seeds of Brucea javanicaon Hela cell |
| title_sort | spectroscopic analysis and cytotoxic activity of quas sinoid isolated from the seeds of brucea javanicaon hela cell |
| topic | Quassinoid B. javanica Brusein A Spectroscopic Anticancer |
| url | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/604/479 |
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