Influence of targeted inhibition of DNAJC1 gene on apoptosis and proliferation in multiple myeloma cells
Objective To determine the effect of DNAJC1 gene on the proliferation and apoptosis of multiple myeloma cell lines and preliminarily investigate its mechanism. Methods DNAJC1-siRNA and its negative control (siRNA) were transfected into human multiple myeloma cell line U266 respectively. The interfer...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Third Military Medical University
2021-10-01
|
| Series: | Di-san junyi daxue xuebao |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202104054.htm |
| _version_ | 1818606655063982080 |
|---|---|
| author | YANG Bihui HU Guoyu SHEN Chanjuan CHEN Zhenzhen CHEN Haimei LIU Zhao |
| author_facet | YANG Bihui HU Guoyu SHEN Chanjuan CHEN Zhenzhen CHEN Haimei LIU Zhao |
| author_sort | YANG Bihui |
| collection | DOAJ |
| description | Objective To determine the effect of DNAJC1 gene on the proliferation and apoptosis of multiple myeloma cell lines and preliminarily investigate its mechanism. Methods DNAJC1-siRNA and its negative control (siRNA) were transfected into human multiple myeloma cell line U266 respectively. The interference efficiency was determined by RT-qPCR and Western blotting, and cell proliferation and apoptotic rate were measured with CCK-8 assay and flow cytometry, respectively. In addition, RT-qPCR and Western blotting were also used to examine the mRNA and protein expression of PTEN in U266 cells. Results The mRNA and protein levels of DNAJC1 were downregulated in the interference group. The proliferation of U266 cells was inhibited significantly in the interference group, with an inhibitory rate of (58.95±8.13)%, apparently higher than that of the negative siRNA group (P < 0.05). The apoptotic rate was (44.95±6.06)% in the interference group, which notably exceeded that of the negative siRNA group and the control group (P < 0.05). The mRNA and protein levels of PTEN were significantly increased in the interference group (P < 0.05). Conclusion Targeted inhibition of DNAJC1 gene can promote the apoptosis and inhibit the proliferation in multiple myeloma cells, which may be mediated by up-regulation of PTEN. |
| first_indexed | 2024-12-16T14:14:18Z |
| format | Article |
| id | doaj.art-c12619965d934d9e8648fde5fba43bfa |
| institution | Directory Open Access Journal |
| issn | 1000-5404 |
| language | zho |
| last_indexed | 2024-12-16T14:14:18Z |
| publishDate | 2021-10-01 |
| publisher | Editorial Office of Journal of Third Military Medical University |
| record_format | Article |
| series | Di-san junyi daxue xuebao |
| spelling | doaj.art-c12619965d934d9e8648fde5fba43bfa2022-12-21T22:28:39ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042021-10-0143191894189810.16016/j.1000-5404.202104054Influence of targeted inhibition of DNAJC1 gene on apoptosis and proliferation in multiple myeloma cellsYANG Bihui0HU Guoyu1SHEN Chanjuan2CHEN Zhenzhen3 CHEN Haimei4LIU Zhao5 Department of Hematology, Zhuzhou Central Hospital, Zhuzhou, Hunan Province, 412007, China Department of Hematology, Zhuzhou Central Hospital, Zhuzhou, Hunan Province, 412007, China Department of Hematology, Zhuzhou Central Hospital, Zhuzhou, Hunan Province, 412007, China Department of Hematology, Zhuzhou Central Hospital, Zhuzhou, Hunan Province, 412007, China Department of Hematology, Zhuzhou Central Hospital, Zhuzhou, Hunan Province, 412007, ChinaDepartment of Critical Care Medicine, Zhuzhou Central Hospital, Zhuzhou, Hunan Province, 412007, ChinaObjective To determine the effect of DNAJC1 gene on the proliferation and apoptosis of multiple myeloma cell lines and preliminarily investigate its mechanism. Methods DNAJC1-siRNA and its negative control (siRNA) were transfected into human multiple myeloma cell line U266 respectively. The interference efficiency was determined by RT-qPCR and Western blotting, and cell proliferation and apoptotic rate were measured with CCK-8 assay and flow cytometry, respectively. In addition, RT-qPCR and Western blotting were also used to examine the mRNA and protein expression of PTEN in U266 cells. Results The mRNA and protein levels of DNAJC1 were downregulated in the interference group. The proliferation of U266 cells was inhibited significantly in the interference group, with an inhibitory rate of (58.95±8.13)%, apparently higher than that of the negative siRNA group (P < 0.05). The apoptotic rate was (44.95±6.06)% in the interference group, which notably exceeded that of the negative siRNA group and the control group (P < 0.05). The mRNA and protein levels of PTEN were significantly increased in the interference group (P < 0.05). Conclusion Targeted inhibition of DNAJC1 gene can promote the apoptosis and inhibit the proliferation in multiple myeloma cells, which may be mediated by up-regulation of PTEN.http://aammt.tmmu.edu.cn/Upload/rhtml/202104054.htmdnajc1 genemultiple myelomacell apoptosiscell proliferationpten |
| spellingShingle | YANG Bihui HU Guoyu SHEN Chanjuan CHEN Zhenzhen CHEN Haimei LIU Zhao Influence of targeted inhibition of DNAJC1 gene on apoptosis and proliferation in multiple myeloma cells Di-san junyi daxue xuebao dnajc1 gene multiple myeloma cell apoptosis cell proliferation pten |
| title | Influence of targeted inhibition of DNAJC1 gene on apoptosis and proliferation in multiple myeloma cells |
| title_full | Influence of targeted inhibition of DNAJC1 gene on apoptosis and proliferation in multiple myeloma cells |
| title_fullStr | Influence of targeted inhibition of DNAJC1 gene on apoptosis and proliferation in multiple myeloma cells |
| title_full_unstemmed | Influence of targeted inhibition of DNAJC1 gene on apoptosis and proliferation in multiple myeloma cells |
| title_short | Influence of targeted inhibition of DNAJC1 gene on apoptosis and proliferation in multiple myeloma cells |
| title_sort | influence of targeted inhibition of dnajc1 gene on apoptosis and proliferation in multiple myeloma cells |
| topic | dnajc1 gene multiple myeloma cell apoptosis cell proliferation pten |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202104054.htm |
| work_keys_str_mv | AT yangbihui influenceoftargetedinhibitionofdnajc1geneonapoptosisandproliferationinmultiplemyelomacells AT huguoyu influenceoftargetedinhibitionofdnajc1geneonapoptosisandproliferationinmultiplemyelomacells AT shenchanjuan influenceoftargetedinhibitionofdnajc1geneonapoptosisandproliferationinmultiplemyelomacells AT chenzhenzhen influenceoftargetedinhibitionofdnajc1geneonapoptosisandproliferationinmultiplemyelomacells AT chenhaimei influenceoftargetedinhibitionofdnajc1geneonapoptosisandproliferationinmultiplemyelomacells AT liuzhao influenceoftargetedinhibitionofdnajc1geneonapoptosisandproliferationinmultiplemyelomacells |