Plant 45S rDNA clusters are fragile sites and their instability is associated with epigenetic alterations.
Our previous study demonstrated that 45S ribosomal DNA (45S rDNA) clusters were chromosome fragile sites expressed spontaneously in Lolium. In this study, fragile phenotypes of 45S rDNA were observed under aphidicolin (APH) incubation in several plant species. Further actinomycin D (ActD) treatment...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3324429?pdf=render |
| _version_ | 1828850199344185344 |
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| author | Min Huang Hui Li Lu Zhang Fei Gao Pu Wang Yong Hu Shihan Yan Lin Zhao Qi Zhang Junjun Tan Xincheng Liu Shibin He Lijia Li |
| author_facet | Min Huang Hui Li Lu Zhang Fei Gao Pu Wang Yong Hu Shihan Yan Lin Zhao Qi Zhang Junjun Tan Xincheng Liu Shibin He Lijia Li |
| author_sort | Min Huang |
| collection | DOAJ |
| description | Our previous study demonstrated that 45S ribosomal DNA (45S rDNA) clusters were chromosome fragile sites expressed spontaneously in Lolium. In this study, fragile phenotypes of 45S rDNA were observed under aphidicolin (APH) incubation in several plant species. Further actinomycin D (ActD) treatment showed that transcriptional stress might interfere with chromatin packaging, resulting in 45S rDNA fragile expression. These data identified 45S rDNA sites as replication-dependent as well as transcription-dependent fragile sites in plants. In the presence of ActD, a dramatic switch to an open chromatin conformation and accumulated incomplete 5' end of the external transcribed spacer (5'ETS) transcripts were observed, accompanied by decreased DNA methylation, decreased levels of histone H3, and increased histone acetylation and levels of H3K4me2, suggesting that these epigenetic alterations are associated with failure of 45S rDNA condensation. Furthermore, the finding that γ-H2AX was accumulated at 45S rDNA sites following ActD treatment suggested that the DNA damage signaling pathway was associated with the appearance of 45S rDNA fragile phenotypes. Our data provide a link between 45S rDNA transcription and chromatin-packaging defects and open the door for further identifying the molecular mechanism involved. |
| first_indexed | 2024-12-12T23:06:55Z |
| format | Article |
| id | doaj.art-c1287c4ece114205ab69ffbdffe22d2e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T23:06:55Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-c1287c4ece114205ab69ffbdffe22d2e2022-12-22T00:08:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3513910.1371/journal.pone.0035139Plant 45S rDNA clusters are fragile sites and their instability is associated with epigenetic alterations.Min HuangHui LiLu ZhangFei GaoPu WangYong HuShihan YanLin ZhaoQi ZhangJunjun TanXincheng LiuShibin HeLijia LiOur previous study demonstrated that 45S ribosomal DNA (45S rDNA) clusters were chromosome fragile sites expressed spontaneously in Lolium. In this study, fragile phenotypes of 45S rDNA were observed under aphidicolin (APH) incubation in several plant species. Further actinomycin D (ActD) treatment showed that transcriptional stress might interfere with chromatin packaging, resulting in 45S rDNA fragile expression. These data identified 45S rDNA sites as replication-dependent as well as transcription-dependent fragile sites in plants. In the presence of ActD, a dramatic switch to an open chromatin conformation and accumulated incomplete 5' end of the external transcribed spacer (5'ETS) transcripts were observed, accompanied by decreased DNA methylation, decreased levels of histone H3, and increased histone acetylation and levels of H3K4me2, suggesting that these epigenetic alterations are associated with failure of 45S rDNA condensation. Furthermore, the finding that γ-H2AX was accumulated at 45S rDNA sites following ActD treatment suggested that the DNA damage signaling pathway was associated with the appearance of 45S rDNA fragile phenotypes. Our data provide a link between 45S rDNA transcription and chromatin-packaging defects and open the door for further identifying the molecular mechanism involved.http://europepmc.org/articles/PMC3324429?pdf=render |
| spellingShingle | Min Huang Hui Li Lu Zhang Fei Gao Pu Wang Yong Hu Shihan Yan Lin Zhao Qi Zhang Junjun Tan Xincheng Liu Shibin He Lijia Li Plant 45S rDNA clusters are fragile sites and their instability is associated with epigenetic alterations. PLoS ONE |
| title | Plant 45S rDNA clusters are fragile sites and their instability is associated with epigenetic alterations. |
| title_full | Plant 45S rDNA clusters are fragile sites and their instability is associated with epigenetic alterations. |
| title_fullStr | Plant 45S rDNA clusters are fragile sites and their instability is associated with epigenetic alterations. |
| title_full_unstemmed | Plant 45S rDNA clusters are fragile sites and their instability is associated with epigenetic alterations. |
| title_short | Plant 45S rDNA clusters are fragile sites and their instability is associated with epigenetic alterations. |
| title_sort | plant 45s rdna clusters are fragile sites and their instability is associated with epigenetic alterations |
| url | http://europepmc.org/articles/PMC3324429?pdf=render |
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