Nanoscale organization of rotavirus replication machineries
Rotavirus genome replication and assembly take place in cytoplasmic electron dense inclusions termed viroplasms (VPs). Previous conventional optical microscopy studies observing the intracellular distribution of rotavirus proteins and their organization in VPs have lacked molecular-scale spatial res...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2019-07-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/42906 |
| _version_ | 1818019893401878528 |
|---|---|
| author | Yasel Garcés Suárez Jose L Martínez David Torres Hernández Haydee Olinca Hernández Arianna Pérez-Delgado Mayra Méndez Christopher D Wood Juan Manuel Rendon-Mancha Daniela Silva-Ayala Susana López Adán Guerrero Carlos F Arias |
| author_facet | Yasel Garcés Suárez Jose L Martínez David Torres Hernández Haydee Olinca Hernández Arianna Pérez-Delgado Mayra Méndez Christopher D Wood Juan Manuel Rendon-Mancha Daniela Silva-Ayala Susana López Adán Guerrero Carlos F Arias |
| author_sort | Yasel Garcés Suárez |
| collection | DOAJ |
| description | Rotavirus genome replication and assembly take place in cytoplasmic electron dense inclusions termed viroplasms (VPs). Previous conventional optical microscopy studies observing the intracellular distribution of rotavirus proteins and their organization in VPs have lacked molecular-scale spatial resolution, due to inherent spatial resolution constraints. In this work we employed super-resolution microscopy to reveal the nanometric-scale organization of VPs formed during rotavirus infection, and quantitatively describe the structural organization of seven viral proteins within and around the VPs. The observed viral components are spatially organized as five concentric layers, in which NSP5 localizes at the center of the VPs, surrounded by a layer of NSP2 and NSP4 proteins, followed by an intermediate zone comprised of the VP1, VP2, VP6. In the outermost zone, we observed a ring of VP4 and finally a layer of VP7. These findings show that rotavirus VPs are highly organized organelles. |
| first_indexed | 2024-04-14T07:58:46Z |
| format | Article |
| id | doaj.art-c129fcc9fff14d5cafa052c4e2cb1670 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:58:46Z |
| publishDate | 2019-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c129fcc9fff14d5cafa052c4e2cb16702022-12-22T02:04:58ZengeLife Sciences Publications LtdeLife2050-084X2019-07-01810.7554/eLife.42906Nanoscale organization of rotavirus replication machineriesYasel Garcés Suárez0https://orcid.org/0000-0001-8401-8352Jose L Martínez1https://orcid.org/0000-0002-0529-7558David Torres Hernández2Haydee Olinca Hernández3Arianna Pérez-Delgado4Mayra Méndez5Christopher D Wood6Juan Manuel Rendon-Mancha7https://orcid.org/0000-0002-9629-7050Daniela Silva-Ayala8Susana López9Adán Guerrero10https://orcid.org/0000-0002-4389-5516Carlos F Arias11https://orcid.org/0000-0003-3130-4501Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, Mexico; Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, Mexico; Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, MexicoCentro de Investigación en Ciencias, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, Mexico; Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, MexicoCentro de Investigación en Ciencias, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, Mexico; Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, MexicoRotavirus genome replication and assembly take place in cytoplasmic electron dense inclusions termed viroplasms (VPs). Previous conventional optical microscopy studies observing the intracellular distribution of rotavirus proteins and their organization in VPs have lacked molecular-scale spatial resolution, due to inherent spatial resolution constraints. In this work we employed super-resolution microscopy to reveal the nanometric-scale organization of VPs formed during rotavirus infection, and quantitatively describe the structural organization of seven viral proteins within and around the VPs. The observed viral components are spatially organized as five concentric layers, in which NSP5 localizes at the center of the VPs, surrounded by a layer of NSP2 and NSP4 proteins, followed by an intermediate zone comprised of the VP1, VP2, VP6. In the outermost zone, we observed a ring of VP4 and finally a layer of VP7. These findings show that rotavirus VPs are highly organized organelles.https://elifesciences.org/articles/42906super resolution microscopyrotavirusviroplasms |
| spellingShingle | Yasel Garcés Suárez Jose L Martínez David Torres Hernández Haydee Olinca Hernández Arianna Pérez-Delgado Mayra Méndez Christopher D Wood Juan Manuel Rendon-Mancha Daniela Silva-Ayala Susana López Adán Guerrero Carlos F Arias Nanoscale organization of rotavirus replication machineries eLife super resolution microscopy rotavirus viroplasms |
| title | Nanoscale organization of rotavirus replication machineries |
| title_full | Nanoscale organization of rotavirus replication machineries |
| title_fullStr | Nanoscale organization of rotavirus replication machineries |
| title_full_unstemmed | Nanoscale organization of rotavirus replication machineries |
| title_short | Nanoscale organization of rotavirus replication machineries |
| title_sort | nanoscale organization of rotavirus replication machineries |
| topic | super resolution microscopy rotavirus viroplasms |
| url | https://elifesciences.org/articles/42906 |
| work_keys_str_mv | AT yaselgarcessuarez nanoscaleorganizationofrotavirusreplicationmachineries AT joselmartinez nanoscaleorganizationofrotavirusreplicationmachineries AT davidtorreshernandez nanoscaleorganizationofrotavirusreplicationmachineries AT haydeeolincahernandez nanoscaleorganizationofrotavirusreplicationmachineries AT ariannaperezdelgado nanoscaleorganizationofrotavirusreplicationmachineries AT mayramendez nanoscaleorganizationofrotavirusreplicationmachineries AT christopherdwood nanoscaleorganizationofrotavirusreplicationmachineries AT juanmanuelrendonmancha nanoscaleorganizationofrotavirusreplicationmachineries AT danielasilvaayala nanoscaleorganizationofrotavirusreplicationmachineries AT susanalopez nanoscaleorganizationofrotavirusreplicationmachineries AT adanguerrero nanoscaleorganizationofrotavirusreplicationmachineries AT carlosfarias nanoscaleorganizationofrotavirusreplicationmachineries |