Ceftriaxone-Loaded Polymeric Microneedles, Dressings, and Microfibers for Wound Treatment
The objective of this study was to create polymeric dressings, microfibers, and microneedles (MN) loaded with ceftriaxone, using PMVA (Poly (Methyl vinyl ether-alt-maleic acid), Kollicoat<sup>®</sup> 100P, and Kollicoat<sup>®</sup> Protect as polymers to treat diabetic wounds...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
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| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/15/12/2610 |
| _version_ | 1797592937194848256 |
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| author | Pablo Serrano-Castañeda Miguel Alejandro Ochoa Loyo Cristian Ezequiel Tinoco Hernández Brian Miguel Anaya-Ortega Omar Rodrigo Guadarrama-Escobar Ericka Anguiano-Almazán Betsabé Rodríguez-Pérez Ma. Concepción Peña-Juárez Alma Vázquez-Durán Abraham Méndez-Albores Isabel Marlen Rodríguez-Cruz Miriam Isabel Morales-Florido José Juan Escobar-Chávez |
| author_facet | Pablo Serrano-Castañeda Miguel Alejandro Ochoa Loyo Cristian Ezequiel Tinoco Hernández Brian Miguel Anaya-Ortega Omar Rodrigo Guadarrama-Escobar Ericka Anguiano-Almazán Betsabé Rodríguez-Pérez Ma. Concepción Peña-Juárez Alma Vázquez-Durán Abraham Méndez-Albores Isabel Marlen Rodríguez-Cruz Miriam Isabel Morales-Florido José Juan Escobar-Chávez |
| author_sort | Pablo Serrano-Castañeda |
| collection | DOAJ |
| description | The objective of this study was to create polymeric dressings, microfibers, and microneedles (MN) loaded with ceftriaxone, using PMVA (Poly (Methyl vinyl ether-alt-maleic acid), Kollicoat<sup>®</sup> 100P, and Kollicoat<sup>®</sup> Protect as polymers to treat diabetic wounds and accelerate their recovery. These formulations were optimized through a series of experiments and were subsequently subjected to physicochemical tests. The results of the characterization of the dressings, microfibers, and microneedles (PMVA and 100P) were, respectively, a bioadhesion of 281.34, 720, 720, 2487, and 510.5 gf; a post-humectation bioadhesion of 186.34, 831.5, 2380, and 630.5 gf, tear strength of 2200, 1233, 1562, and 385 gf, erythema of 358, 8.4, 227, and 188; transepidermal water loss (TEWL) of 2.6, 4.7, 1.9, and 5.2 g/h·m<sup>2</sup>; hydration of 76.1, 89.9, 73.5, and 83.5%; pH of 4.85, 5.40, 5.85, and 4.85; and drug release (Peppas kinetics release) of n: 0.53, n: 0.62, n: 0.62, and n: 0.66). In vitro studies were performed on Franz-type diffusion cells and indicated flux of 57.1, 145.4, 718.7, and 2.7 µg/cm<sup>2</sup>; permeation coefficient (Kp) of 13.2, 19.56, 42, and 0.00015 cm<sup>2</sup>/h; and time lag (t<sub>L</sub>) of 6.29, 17.61, 27. 49, and 22.3 h, respectively, in wounded skin. There was no passage of ceftriaxone from dressings and microfibers to healthy skin, but that was not the case for PMVA/100P and Kollicoat<sup>®</sup> 100P microneedles, which exhibited flux of 194 and 0.4 µg/cm<sup>2</sup>, Kp of 11.3 and 0.00002 cm<sup>2</sup>/h, and t<sub>L</sub> of 5.2 and 9.7 h, respectively. The healing time of the formulations in vivo (tests carried out using diabetic Wistar rats) was under 14 days. In summary, polymeric dressings, microfibers, and microneedles loaded with ceftriaxone were developed. These formulations have the potential to address the challenges associated with chronic wounds, such as diabetic foot, improving the outcomes. |
| first_indexed | 2024-03-11T02:01:20Z |
| format | Article |
| id | doaj.art-c12c127de6de44a2865553765eba8ca4 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T02:01:20Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-c12c127de6de44a2865553765eba8ca42023-11-18T12:12:09ZengMDPI AGPolymers2073-43602023-06-011512261010.3390/polym15122610Ceftriaxone-Loaded Polymeric Microneedles, Dressings, and Microfibers for Wound TreatmentPablo Serrano-Castañeda0Miguel Alejandro Ochoa Loyo1Cristian Ezequiel Tinoco Hernández2Brian Miguel Anaya-Ortega3Omar Rodrigo Guadarrama-Escobar4Ericka Anguiano-Almazán5Betsabé Rodríguez-Pérez6Ma. Concepción Peña-Juárez7Alma Vázquez-Durán8Abraham Méndez-Albores9Isabel Marlen Rodríguez-Cruz10Miriam Isabel Morales-Florido11José Juan Escobar-Chávez12Unidad de Investigación Multidisciplinaria-Lab 12, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carretera Cuautitlán-Teoloyucan, km 2.5 San Sebastián Xhala, Cuautitlán Izcalli 54714, MexicoUnidad de Investigación Multidisciplinaria-Lab 12, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carretera Cuautitlán-Teoloyucan, km 2.5 San Sebastián Xhala, Cuautitlán Izcalli 54714, MexicoUnidad de Investigación Multidisciplinaria-Lab 12, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carretera Cuautitlán-Teoloyucan, km 2.5 San Sebastián Xhala, Cuautitlán Izcalli 54714, MexicoUnidad de Investigación Multidisciplinaria-Lab 12, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carretera Cuautitlán-Teoloyucan, km 2.5 San Sebastián Xhala, Cuautitlán Izcalli 54714, MexicoUnidad de Investigación Multidisciplinaria-Lab 12, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carretera Cuautitlán-Teoloyucan, km 2.5 San Sebastián Xhala, Cuautitlán Izcalli 54714, MexicoUnidad de Investigación Multidisciplinaria-Lab 12, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carretera Cuautitlán-Teoloyucan, km 2.5 San Sebastián Xhala, Cuautitlán Izcalli 54714, MexicoLaboratorio de Servicio de Análisis de Propóleos (LASAP), Unidad de Investigación Multidisciplinaria (UIM), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Cuautitlán Izcalli 54714, MexicoUnidad de Investigación Multidisciplinaria-Lab 12, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carretera Cuautitlán-Teoloyucan, km 2.5 San Sebastián Xhala, Cuautitlán Izcalli 54714, MexicoUnidad de Investigación Multidisciplinaria L14 (Ciencia y Tecnología de los Materiales), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Cuautitlán Izcalli 54714, MexicoUnidad de Investigación Multidisciplinaria L14 (Ciencia y Tecnología de los Materiales), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Cuautitlán Izcalli 54714, MexicoUnidad de Enseñanza e Investigación, Hospital Regional de Alta Especialidad de Zumpango, Carretera Zumpango-Jilotzingo #400, Barrio de Santiago, 2ª Sección, Zumpango 55600, MexicoUnidad de Investigación Multidisciplinaria-Lab 12, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carretera Cuautitlán-Teoloyucan, km 2.5 San Sebastián Xhala, Cuautitlán Izcalli 54714, MexicoUnidad de Investigación Multidisciplinaria-Lab 12, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carretera Cuautitlán-Teoloyucan, km 2.5 San Sebastián Xhala, Cuautitlán Izcalli 54714, MexicoThe objective of this study was to create polymeric dressings, microfibers, and microneedles (MN) loaded with ceftriaxone, using PMVA (Poly (Methyl vinyl ether-alt-maleic acid), Kollicoat<sup>®</sup> 100P, and Kollicoat<sup>®</sup> Protect as polymers to treat diabetic wounds and accelerate their recovery. These formulations were optimized through a series of experiments and were subsequently subjected to physicochemical tests. The results of the characterization of the dressings, microfibers, and microneedles (PMVA and 100P) were, respectively, a bioadhesion of 281.34, 720, 720, 2487, and 510.5 gf; a post-humectation bioadhesion of 186.34, 831.5, 2380, and 630.5 gf, tear strength of 2200, 1233, 1562, and 385 gf, erythema of 358, 8.4, 227, and 188; transepidermal water loss (TEWL) of 2.6, 4.7, 1.9, and 5.2 g/h·m<sup>2</sup>; hydration of 76.1, 89.9, 73.5, and 83.5%; pH of 4.85, 5.40, 5.85, and 4.85; and drug release (Peppas kinetics release) of n: 0.53, n: 0.62, n: 0.62, and n: 0.66). In vitro studies were performed on Franz-type diffusion cells and indicated flux of 57.1, 145.4, 718.7, and 2.7 µg/cm<sup>2</sup>; permeation coefficient (Kp) of 13.2, 19.56, 42, and 0.00015 cm<sup>2</sup>/h; and time lag (t<sub>L</sub>) of 6.29, 17.61, 27. 49, and 22.3 h, respectively, in wounded skin. There was no passage of ceftriaxone from dressings and microfibers to healthy skin, but that was not the case for PMVA/100P and Kollicoat<sup>®</sup> 100P microneedles, which exhibited flux of 194 and 0.4 µg/cm<sup>2</sup>, Kp of 11.3 and 0.00002 cm<sup>2</sup>/h, and t<sub>L</sub> of 5.2 and 9.7 h, respectively. The healing time of the formulations in vivo (tests carried out using diabetic Wistar rats) was under 14 days. In summary, polymeric dressings, microfibers, and microneedles loaded with ceftriaxone were developed. These formulations have the potential to address the challenges associated with chronic wounds, such as diabetic foot, improving the outcomes.https://www.mdpi.com/2073-4360/15/12/2610microneedlesmicrofibersdressingswound treatmentceftriaxoneKollicoat<sup>®</sup> Protect |
| spellingShingle | Pablo Serrano-Castañeda Miguel Alejandro Ochoa Loyo Cristian Ezequiel Tinoco Hernández Brian Miguel Anaya-Ortega Omar Rodrigo Guadarrama-Escobar Ericka Anguiano-Almazán Betsabé Rodríguez-Pérez Ma. Concepción Peña-Juárez Alma Vázquez-Durán Abraham Méndez-Albores Isabel Marlen Rodríguez-Cruz Miriam Isabel Morales-Florido José Juan Escobar-Chávez Ceftriaxone-Loaded Polymeric Microneedles, Dressings, and Microfibers for Wound Treatment Polymers microneedles microfibers dressings wound treatment ceftriaxone Kollicoat<sup>®</sup> Protect |
| title | Ceftriaxone-Loaded Polymeric Microneedles, Dressings, and Microfibers for Wound Treatment |
| title_full | Ceftriaxone-Loaded Polymeric Microneedles, Dressings, and Microfibers for Wound Treatment |
| title_fullStr | Ceftriaxone-Loaded Polymeric Microneedles, Dressings, and Microfibers for Wound Treatment |
| title_full_unstemmed | Ceftriaxone-Loaded Polymeric Microneedles, Dressings, and Microfibers for Wound Treatment |
| title_short | Ceftriaxone-Loaded Polymeric Microneedles, Dressings, and Microfibers for Wound Treatment |
| title_sort | ceftriaxone loaded polymeric microneedles dressings and microfibers for wound treatment |
| topic | microneedles microfibers dressings wound treatment ceftriaxone Kollicoat<sup>®</sup> Protect |
| url | https://www.mdpi.com/2073-4360/15/12/2610 |
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