Red blood cell rheology during a complete blood count: A proof of concept.
Counting and sizing blood cells in hematological analyzers is achieved using the Coulter principle. The cells flow in a micro-aperture in which a strong electrical field is imposed, so that an electrical perturbation, called pulse, is measured each time a cell crosses the orifice. The pulses are exp...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0280952 |
| _version_ | 1797937996181274624 |
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| author | Pierre Taraconat Jean-Philippe Gineys Damien Isebe Franck Nicoud Simon Mendez |
| author_facet | Pierre Taraconat Jean-Philippe Gineys Damien Isebe Franck Nicoud Simon Mendez |
| author_sort | Pierre Taraconat |
| collection | DOAJ |
| description | Counting and sizing blood cells in hematological analyzers is achieved using the Coulter principle. The cells flow in a micro-aperture in which a strong electrical field is imposed, so that an electrical perturbation, called pulse, is measured each time a cell crosses the orifice. The pulses are expected to contain information on the shape and deformability of Red Blood Cells (RBCs), since recent studies state that RBCs rotate and deform in the micro-orifice. By implementing a dedicated numerical model, the present study sheds light on a variety of cells dynamics, which leads to different associated pulse signatures. Furthermore, simulations provide new insights on how RBCs shapes and mechanical properties affect the measured signals. Those numerical observations are confirmed by experimental assays. Finally, specific features are introduced for assessing the most relevant characteristics from the various pulse signatures and shown to highlight RBCs alterations induced by drugs. In summary, this study paves the way to a characterization of RBC rheology by routine hematological instruments. |
| first_indexed | 2024-04-10T18:52:55Z |
| format | Article |
| id | doaj.art-c12d2cdd5e50479bb7587cba4be5b32f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T18:52:55Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c12d2cdd5e50479bb7587cba4be5b32f2023-02-01T05:31:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01181e028095210.1371/journal.pone.0280952Red blood cell rheology during a complete blood count: A proof of concept.Pierre TaraconatJean-Philippe GineysDamien IsebeFranck NicoudSimon MendezCounting and sizing blood cells in hematological analyzers is achieved using the Coulter principle. The cells flow in a micro-aperture in which a strong electrical field is imposed, so that an electrical perturbation, called pulse, is measured each time a cell crosses the orifice. The pulses are expected to contain information on the shape and deformability of Red Blood Cells (RBCs), since recent studies state that RBCs rotate and deform in the micro-orifice. By implementing a dedicated numerical model, the present study sheds light on a variety of cells dynamics, which leads to different associated pulse signatures. Furthermore, simulations provide new insights on how RBCs shapes and mechanical properties affect the measured signals. Those numerical observations are confirmed by experimental assays. Finally, specific features are introduced for assessing the most relevant characteristics from the various pulse signatures and shown to highlight RBCs alterations induced by drugs. In summary, this study paves the way to a characterization of RBC rheology by routine hematological instruments.https://doi.org/10.1371/journal.pone.0280952 |
| spellingShingle | Pierre Taraconat Jean-Philippe Gineys Damien Isebe Franck Nicoud Simon Mendez Red blood cell rheology during a complete blood count: A proof of concept. PLoS ONE |
| title | Red blood cell rheology during a complete blood count: A proof of concept. |
| title_full | Red blood cell rheology during a complete blood count: A proof of concept. |
| title_fullStr | Red blood cell rheology during a complete blood count: A proof of concept. |
| title_full_unstemmed | Red blood cell rheology during a complete blood count: A proof of concept. |
| title_short | Red blood cell rheology during a complete blood count: A proof of concept. |
| title_sort | red blood cell rheology during a complete blood count a proof of concept |
| url | https://doi.org/10.1371/journal.pone.0280952 |
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