Purification and Characterization of a Fibrinolytic Enzyme from Snake Venom of Macrovipera lebetina turanica (
Objectives: Fibrinolytic enzyme preparations were isolated from the snake venom of Macrovipera lebetica turanica in this study. Methods: The purity of the preparations was determined using SDS-PAGE and the enzymic characteristics of the purified fibrinolytic enzyme were determined. Results: 1. All...
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| Format: | Article |
| Language: | English |
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Korean Pharmacopuncture Institute
2011-06-01
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| Series: | Journal of Pharmacopuncture |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3831/KPI.2011.14.2.005 |
| _version_ | 1818473294594048000 |
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| author | Kwon Ki-Rok Park Do-Il Lee Seung-Bae Choi Suk-Ho |
| author_facet | Kwon Ki-Rok Park Do-Il Lee Seung-Bae Choi Suk-Ho |
| author_sort | Kwon Ki-Rok |
| collection | DOAJ |
| description | Objectives: Fibrinolytic enzyme preparations were isolated from the snake venom of Macrovipera lebetica turanica in this study.
Methods: The purity of the preparations was determined using SDS-PAGE and the enzymic characteristics of the purified fibrinolytic enzyme were determined.
Results: 1. All of the two preparations with fibrinolytic activity obtained from the snake venom of M. l. turanicat contained the major polypeptide with the molecular weight of 27,500. One of the preparation showed purified fibrinolytic enzyme.
2. The purified fibrinolytic enzyme hydrolyzed α-chain of fibrinogen faster than β-chain but not γ-chain.
3. The fibrinolytic activity was inhibited completely by EDTA, EGTA, 1,10-phenanthroline, and dithiothreitol.
4. The fibrinolytic activity was inhibited completely by calcium chloride, iron(III) chloride, mercuric chloride, and cobalt (II) chloride.
5. The fibrinolysis zone formed after addition of zinc sulfate was smaller but clearer than the control.
Conclusions: These results suggested that the fibrinolytic enzyme purifed from the snake venom of M. l turanica was a metalloprotease containing dithiol group. |
| first_indexed | 2024-04-14T04:22:18Z |
| format | Article |
| id | doaj.art-c12de42b7b5a4bd4bd626b8fa9954b3f |
| institution | Directory Open Access Journal |
| issn | 2093-6966 2234-6856 |
| language | English |
| last_indexed | 2024-04-14T04:22:18Z |
| publishDate | 2011-06-01 |
| publisher | Korean Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Pharmacopuncture |
| spelling | doaj.art-c12de42b7b5a4bd4bd626b8fa9954b3f2022-12-22T02:12:29ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562011-06-0114251410.3831/KPI.2011.14.2.005Purification and Characterization of a Fibrinolytic Enzyme from Snake Venom of Macrovipera lebetina turanica (Kwon Ki-Rok0Park Do-Il 1Lee Seung-Bae2Choi Suk-Ho 3Graduate School of Oriental Medicine, Sangji UniversityGraduate School of Oriental Medicine, Sangji UniversityGraduate School of Oriental Medicine, Sangji UniversityGraduate School of Oriental Medicine, Sangji UniversityObjectives: Fibrinolytic enzyme preparations were isolated from the snake venom of Macrovipera lebetica turanica in this study. Methods: The purity of the preparations was determined using SDS-PAGE and the enzymic characteristics of the purified fibrinolytic enzyme were determined. Results: 1. All of the two preparations with fibrinolytic activity obtained from the snake venom of M. l. turanicat contained the major polypeptide with the molecular weight of 27,500. One of the preparation showed purified fibrinolytic enzyme. 2. The purified fibrinolytic enzyme hydrolyzed α-chain of fibrinogen faster than β-chain but not γ-chain. 3. The fibrinolytic activity was inhibited completely by EDTA, EGTA, 1,10-phenanthroline, and dithiothreitol. 4. The fibrinolytic activity was inhibited completely by calcium chloride, iron(III) chloride, mercuric chloride, and cobalt (II) chloride. 5. The fibrinolysis zone formed after addition of zinc sulfate was smaller but clearer than the control. Conclusions: These results suggested that the fibrinolytic enzyme purifed from the snake venom of M. l turanica was a metalloprotease containing dithiol group.http://dx.doi.org/10.3831/KPI.2011.14.2.005Snake venomMacrovipera lebetica turanica. f fibrinolytic enzymefibrinolytic activity |
| spellingShingle | Kwon Ki-Rok Park Do-Il Lee Seung-Bae Choi Suk-Ho Purification and Characterization of a Fibrinolytic Enzyme from Snake Venom of Macrovipera lebetina turanica ( Journal of Pharmacopuncture Snake venom Macrovipera lebetica turanica. f fibrinolytic enzyme fibrinolytic activity |
| title | Purification and Characterization of a Fibrinolytic Enzyme from Snake Venom of Macrovipera lebetina turanica ( |
| title_full | Purification and Characterization of a Fibrinolytic Enzyme from Snake Venom of Macrovipera lebetina turanica ( |
| title_fullStr | Purification and Characterization of a Fibrinolytic Enzyme from Snake Venom of Macrovipera lebetina turanica ( |
| title_full_unstemmed | Purification and Characterization of a Fibrinolytic Enzyme from Snake Venom of Macrovipera lebetina turanica ( |
| title_short | Purification and Characterization of a Fibrinolytic Enzyme from Snake Venom of Macrovipera lebetina turanica ( |
| title_sort | purification and characterization of a fibrinolytic enzyme from snake venom of macrovipera lebetina turanica |
| topic | Snake venom Macrovipera lebetica turanica. f fibrinolytic enzyme fibrinolytic activity |
| url | http://dx.doi.org/10.3831/KPI.2011.14.2.005 |
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