Rutaecarpine exhibits anti-diabetic potential in high fat diet–multiple low dose streptozotocin induced type 2 diabetic mice and in vitro by modulating hepatic glucose homeostasis
Rutaecarpine, an indolopyridoquinazoline alkaloid, attracted attentions because of possessing various biological activities. The objective of this study was to investigate the effect of rutaecarpine on glucose and lipid metabolism in high fat diet–multiple low dose streptozotocin induced type 2 diab...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-08-01
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| Series: | Journal of Pharmacological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861320300578 |
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| author | Laishram Surbala Chingakham Brajakishor Singh Rajkumari Vidyabati Devi Oinam Joychandra Singh |
| author_facet | Laishram Surbala Chingakham Brajakishor Singh Rajkumari Vidyabati Devi Oinam Joychandra Singh |
| author_sort | Laishram Surbala |
| collection | DOAJ |
| description | Rutaecarpine, an indolopyridoquinazoline alkaloid, attracted attentions because of possessing various biological activities. The objective of this study was to investigate the effect of rutaecarpine on glucose and lipid metabolism in high fat diet–multiple low dose streptozotocin induced type 2 diabetic (HFD-db) mice and to understand the mechanism of action. HFD-db mice showed impaired glucose metabolism and lipid profile. Oral administration of rutaecarpine reduced the blood glucose levels, decreased blood hemoglobin A1c (HbA1c) levels, improved glucose tolerance and restored insulin sensitivity in HFD-db mice. Rutaecarpine also decreased body weight gain, water intake and visceral fat gain in HFD-db mice. Total cholesterol, triglycerides, very low density lipoprotein and low density lipoprotein were reduced and high density lipoprotein level was augmented in rutaecarpine treated HFD-db mice. Rutaecarpine also reduced the elevated levels of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, urea and creatinine in HFD-db mice. Rutaecarpine significantly promoted the rate of glucose consumption, glucose uptake and glycolysis in C2C12 myotubes. Western blotting results showed that rutaecarpine augmented p-GSK-3β and p-AMPK expression, and suppressed G6Pase expression in HepG2 cells. These results suggest that rutaecarpine might be having therapeutic importance to fight against type 2 diabetes mellitus associated with dyslipidemia. |
| first_indexed | 2024-12-14T09:51:32Z |
| format | Article |
| id | doaj.art-c12e8fffdf7944588836130b21af2681 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-14T09:51:32Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-c12e8fffdf7944588836130b21af26812022-12-21T23:07:31ZengElsevierJournal of Pharmacological Sciences1347-86132020-08-011434307314Rutaecarpine exhibits anti-diabetic potential in high fat diet–multiple low dose streptozotocin induced type 2 diabetic mice and in vitro by modulating hepatic glucose homeostasisLaishram Surbala0Chingakham Brajakishor Singh1Rajkumari Vidyabati Devi2Oinam Joychandra Singh3Department of Pharmacology, Jawaharlal Nehru Institute of Medical Science, Porompat, Imphal East, 795005, Manipur, IndiaMedicinal Plants and Horticulture Resources Division, Institute of Bioresources and Sustainable Development, Takyelpat, Imphal West, 795001, Manipur, IndiaDepartment of Biochemistry, Jawaharlal Nehru Institute of Medical Science, Porompat, Imphal East, 795005, Manipur, IndiaDepartment of Pharmacology, Jawaharlal Nehru Institute of Medical Science, Porompat, Imphal East, 795005, Manipur, India; Corresponding author.Rutaecarpine, an indolopyridoquinazoline alkaloid, attracted attentions because of possessing various biological activities. The objective of this study was to investigate the effect of rutaecarpine on glucose and lipid metabolism in high fat diet–multiple low dose streptozotocin induced type 2 diabetic (HFD-db) mice and to understand the mechanism of action. HFD-db mice showed impaired glucose metabolism and lipid profile. Oral administration of rutaecarpine reduced the blood glucose levels, decreased blood hemoglobin A1c (HbA1c) levels, improved glucose tolerance and restored insulin sensitivity in HFD-db mice. Rutaecarpine also decreased body weight gain, water intake and visceral fat gain in HFD-db mice. Total cholesterol, triglycerides, very low density lipoprotein and low density lipoprotein were reduced and high density lipoprotein level was augmented in rutaecarpine treated HFD-db mice. Rutaecarpine also reduced the elevated levels of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, urea and creatinine in HFD-db mice. Rutaecarpine significantly promoted the rate of glucose consumption, glucose uptake and glycolysis in C2C12 myotubes. Western blotting results showed that rutaecarpine augmented p-GSK-3β and p-AMPK expression, and suppressed G6Pase expression in HepG2 cells. These results suggest that rutaecarpine might be having therapeutic importance to fight against type 2 diabetes mellitus associated with dyslipidemia.http://www.sciencedirect.com/science/article/pii/S1347861320300578RutaecarpineDiabetesDyslipidemiaGlycolysisGluconeogenesis |
| spellingShingle | Laishram Surbala Chingakham Brajakishor Singh Rajkumari Vidyabati Devi Oinam Joychandra Singh Rutaecarpine exhibits anti-diabetic potential in high fat diet–multiple low dose streptozotocin induced type 2 diabetic mice and in vitro by modulating hepatic glucose homeostasis Journal of Pharmacological Sciences Rutaecarpine Diabetes Dyslipidemia Glycolysis Gluconeogenesis |
| title | Rutaecarpine exhibits anti-diabetic potential in high fat diet–multiple low dose streptozotocin induced type 2 diabetic mice and in vitro by modulating hepatic glucose homeostasis |
| title_full | Rutaecarpine exhibits anti-diabetic potential in high fat diet–multiple low dose streptozotocin induced type 2 diabetic mice and in vitro by modulating hepatic glucose homeostasis |
| title_fullStr | Rutaecarpine exhibits anti-diabetic potential in high fat diet–multiple low dose streptozotocin induced type 2 diabetic mice and in vitro by modulating hepatic glucose homeostasis |
| title_full_unstemmed | Rutaecarpine exhibits anti-diabetic potential in high fat diet–multiple low dose streptozotocin induced type 2 diabetic mice and in vitro by modulating hepatic glucose homeostasis |
| title_short | Rutaecarpine exhibits anti-diabetic potential in high fat diet–multiple low dose streptozotocin induced type 2 diabetic mice and in vitro by modulating hepatic glucose homeostasis |
| title_sort | rutaecarpine exhibits anti diabetic potential in high fat diet multiple low dose streptozotocin induced type 2 diabetic mice and in vitro by modulating hepatic glucose homeostasis |
| topic | Rutaecarpine Diabetes Dyslipidemia Glycolysis Gluconeogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S1347861320300578 |
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