Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris.
The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene. The results showed that the corresponding mutation...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3589435?pdf=render |
| _version_ | 1811218790946439168 |
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| author | Hong Hu Jie Gao Jun He Bing Yu Ping Zheng Zhiqing Huang Xiangbing Mao Jie Yu Guoquan Han Daiwen Chen |
| author_facet | Hong Hu Jie Gao Jun He Bing Yu Ping Zheng Zhiqing Huang Xiangbing Mao Jie Yu Guoquan Han Daiwen Chen |
| author_sort | Hong Hu |
| collection | DOAJ |
| description | The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene. The results showed that the corresponding mutations (synonymous codons) according to the codon bias in Pichia pastoris were successfully introduced into keratinase gene. The highest keratinase activity produced by P. pastoris pPICZαA-kerAwt, pPICZαA-kerAopti1 and pPICZαA-kerAopti2 was 195 U/ml, 324 U/ml and 293 U/ml respectively. In addition, there was no significant difference in biomass concentration, target gene copy numbers and relative mRNA expression levels of every positive strain. The molecular weight of keratinase secreted by recombinant P. pastori was approx. 39 kDa. It was optimally active at pH 7.5 and 50°C. The recombinant keratinase could efficiently degrade both α-keratin (keratin azure) and β-keratin (chicken feather meal). These properties make the P. pastoris pPICZαA-kerAopti1 a suitable candidate for industrial production of keratinases. |
| first_indexed | 2024-04-12T07:15:18Z |
| format | Article |
| id | doaj.art-c12ec24b399d4f7d9c3bc43fcad35989 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T07:15:18Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c12ec24b399d4f7d9c3bc43fcad359892022-12-22T03:42:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5839310.1371/journal.pone.0058393Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris.Hong HuJie GaoJun HeBing YuPing ZhengZhiqing HuangXiangbing MaoJie YuGuoquan HanDaiwen ChenThe main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene. The results showed that the corresponding mutations (synonymous codons) according to the codon bias in Pichia pastoris were successfully introduced into keratinase gene. The highest keratinase activity produced by P. pastoris pPICZαA-kerAwt, pPICZαA-kerAopti1 and pPICZαA-kerAopti2 was 195 U/ml, 324 U/ml and 293 U/ml respectively. In addition, there was no significant difference in biomass concentration, target gene copy numbers and relative mRNA expression levels of every positive strain. The molecular weight of keratinase secreted by recombinant P. pastori was approx. 39 kDa. It was optimally active at pH 7.5 and 50°C. The recombinant keratinase could efficiently degrade both α-keratin (keratin azure) and β-keratin (chicken feather meal). These properties make the P. pastoris pPICZαA-kerAopti1 a suitable candidate for industrial production of keratinases.http://europepmc.org/articles/PMC3589435?pdf=render |
| spellingShingle | Hong Hu Jie Gao Jun He Bing Yu Ping Zheng Zhiqing Huang Xiangbing Mao Jie Yu Guoquan Han Daiwen Chen Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris. PLoS ONE |
| title | Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris. |
| title_full | Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris. |
| title_fullStr | Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris. |
| title_full_unstemmed | Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris. |
| title_short | Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris. |
| title_sort | codon optimization significantly improves the expression level of a keratinase gene in pichia pastoris |
| url | http://europepmc.org/articles/PMC3589435?pdf=render |
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