Proanthocyanidin monomers and cyanidin 3-O-glucoside accumulation in blood-flesh peach (Prunus persica (L.) Batsch) fruit
To better understand the characteristics and mechanisms of proanthocyanidin monomers and anthocyanin synthesis in blood-flesh peach (Prunus persica (L.) Batsch), the accumulation of catechin, epicatechin and cyanidin 3-O-glucoside was determined, and the expression patterns of structural ge...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2017-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641700006Y.pdf |
| _version_ | 1819157505683488768 |
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| author | Yan Juan Cai Zhi-Xiang Shen Zhi-Jun Ma Rui-Juan Yu Ming-Liang |
| author_facet | Yan Juan Cai Zhi-Xiang Shen Zhi-Jun Ma Rui-Juan Yu Ming-Liang |
| author_sort | Yan Juan |
| collection | DOAJ |
| description | To better understand the characteristics and mechanisms of proanthocyanidin
monomers and anthocyanin synthesis in blood-flesh peach (Prunus persica (L.)
Batsch), the accumulation of catechin, epicatechin and cyanidin 3-O-glucoside
was determined, and the expression patterns of structural genes associated
with biosynthesis of those compounds were investigated in the blood-flesh
peach fruit of cultivar “Dahongpao” during fruit development. Our results
show that catechin concentration remained low and comparatively stable
throughout fruit development. The concentration of epicatechin remained low
at the early stages of fruit development and rapidly accumulated during
ripening. Cyanidin 3-O-glucoside was not detected in theearly stages.
Epicatechin started to rapidly accumulate during the ripening period,
reaching a maximum at the mature stage. The expressions of the early and
common genes, phenylalanine ammonia-lyase and chalcone isomerase, were less
associated with proanthocyanidin monomers and cyanidin 3-O-glucoside
accumulation. The expression of other flavonoid ‘early’ biosynthetic genes,
including chalcone synthase (CHS), flavanone 3-hydroxylase, dihydroflavonol
4-reductase (DFR) and leucoanthocyanidin dioxygenase (LDOX), were partly
associated with proanthocyanidin monomers and cyanidin 3-O-glucoside levels,
with expression quantities peaking synchronously at the mature stage.
Leucoanthocyanidin reductase and anthocyanidin reductase, which were the key
genes for proanthocyanidin monomer synthesis, correlated during fruit
development with catechin and epicatechin accumulation respectively;
UDP-glucose: flavonoid 3-O-glucosyltransferase (UGFT), the key gene for
anthocyanin synthesis, was correlated with cyanidin 3-O-glucoside levels. The
synchronous accumulation of epicatechin and cyanidin 3-O-glucoside in
blood-flesh peach could not be explained by the current theory of competitive
distribution mechanism of common substrate. |
| first_indexed | 2024-12-22T16:09:50Z |
| format | Article |
| id | doaj.art-c12eeafc7c1c4acb9fd6b83b686bdad8 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-22T16:09:50Z |
| publishDate | 2017-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-c12eeafc7c1c4acb9fd6b83b686bdad82022-12-21T18:20:31ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392017-01-0169461161710.2298/ABS161212006Y0354-46641700006YProanthocyanidin monomers and cyanidin 3-O-glucoside accumulation in blood-flesh peach (Prunus persica (L.) Batsch) fruitYan Juan0Cai Zhi-Xiang1Shen Zhi-Jun2Ma Rui-Juan3Yu Ming-Liang4Jiangsu Academy of Agricultural Sciences, Institute of Horticulture, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, ChinaJiangsu Academy of Agricultural Sciences, Institute of Horticulture, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, ChinaJiangsu Academy of Agricultural Sciences, Institute of Horticulture, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, ChinaJiangsu Academy of Agricultural Sciences, Institute of Horticulture, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, ChinaJiangsu Academy of Agricultural Sciences, Institute of Horticulture, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, ChinaTo better understand the characteristics and mechanisms of proanthocyanidin monomers and anthocyanin synthesis in blood-flesh peach (Prunus persica (L.) Batsch), the accumulation of catechin, epicatechin and cyanidin 3-O-glucoside was determined, and the expression patterns of structural genes associated with biosynthesis of those compounds were investigated in the blood-flesh peach fruit of cultivar “Dahongpao” during fruit development. Our results show that catechin concentration remained low and comparatively stable throughout fruit development. The concentration of epicatechin remained low at the early stages of fruit development and rapidly accumulated during ripening. Cyanidin 3-O-glucoside was not detected in theearly stages. Epicatechin started to rapidly accumulate during the ripening period, reaching a maximum at the mature stage. The expressions of the early and common genes, phenylalanine ammonia-lyase and chalcone isomerase, were less associated with proanthocyanidin monomers and cyanidin 3-O-glucoside accumulation. The expression of other flavonoid ‘early’ biosynthetic genes, including chalcone synthase (CHS), flavanone 3-hydroxylase, dihydroflavonol 4-reductase (DFR) and leucoanthocyanidin dioxygenase (LDOX), were partly associated with proanthocyanidin monomers and cyanidin 3-O-glucoside levels, with expression quantities peaking synchronously at the mature stage. Leucoanthocyanidin reductase and anthocyanidin reductase, which were the key genes for proanthocyanidin monomer synthesis, correlated during fruit development with catechin and epicatechin accumulation respectively; UDP-glucose: flavonoid 3-O-glucosyltransferase (UGFT), the key gene for anthocyanin synthesis, was correlated with cyanidin 3-O-glucoside levels. The synchronous accumulation of epicatechin and cyanidin 3-O-glucoside in blood-flesh peach could not be explained by the current theory of competitive distribution mechanism of common substrate.http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641700006Y.pdfblood-flesh peach (Prunus persica (L.) Batsch)catechinepicatechincyanidin 3-O-glucosidegene expression |
| spellingShingle | Yan Juan Cai Zhi-Xiang Shen Zhi-Jun Ma Rui-Juan Yu Ming-Liang Proanthocyanidin monomers and cyanidin 3-O-glucoside accumulation in blood-flesh peach (Prunus persica (L.) Batsch) fruit Archives of Biological Sciences blood-flesh peach (Prunus persica (L.) Batsch) catechin epicatechin cyanidin 3-O-glucoside gene expression |
| title | Proanthocyanidin monomers and cyanidin 3-O-glucoside accumulation in blood-flesh peach (Prunus persica (L.) Batsch) fruit |
| title_full | Proanthocyanidin monomers and cyanidin 3-O-glucoside accumulation in blood-flesh peach (Prunus persica (L.) Batsch) fruit |
| title_fullStr | Proanthocyanidin monomers and cyanidin 3-O-glucoside accumulation in blood-flesh peach (Prunus persica (L.) Batsch) fruit |
| title_full_unstemmed | Proanthocyanidin monomers and cyanidin 3-O-glucoside accumulation in blood-flesh peach (Prunus persica (L.) Batsch) fruit |
| title_short | Proanthocyanidin monomers and cyanidin 3-O-glucoside accumulation in blood-flesh peach (Prunus persica (L.) Batsch) fruit |
| title_sort | proanthocyanidin monomers and cyanidin 3 o glucoside accumulation in blood flesh peach prunus persica l batsch fruit |
| topic | blood-flesh peach (Prunus persica (L.) Batsch) catechin epicatechin cyanidin 3-O-glucoside gene expression |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641700006Y.pdf |
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