Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in <i>Bombyx mori</i>
Lepidoptera is one of the most speciose insect orders, causing enormous damage to agricultural and forest crops. Although genome editing has been achieved in a few Lepidoptera for insect controls, most techniques are still limited. Here, by injecting female pupae of the Lepidoptera model species, &l...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/12/932 |
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| author | Bin Yu Sichen Dong Xiaoyu Jiang Liang Qiao Jie Chen Tian Li Guoqing Pan Zeyang Zhou Chunfeng Li |
| author_facet | Bin Yu Sichen Dong Xiaoyu Jiang Liang Qiao Jie Chen Tian Li Guoqing Pan Zeyang Zhou Chunfeng Li |
| author_sort | Bin Yu |
| collection | DOAJ |
| description | Lepidoptera is one of the most speciose insect orders, causing enormous damage to agricultural and forest crops. Although genome editing has been achieved in a few Lepidoptera for insect controls, most techniques are still limited. Here, by injecting female pupae of the Lepidoptera model species, <i>Bombyx mori</i>, gene editing was established using the Receptor-Mediated Ovary Transduction of Cargo (ReMOT) control technique. We identified a <i>B. mori</i> oocytes-targeting peptide ligand (BmOTP, a 29 aa of vitellogenin N-terminal of silkworms) with a highly conserved sequence in lepidopteran insects that could efficiently deliver mCherry into oocytes. When BmOTP was fused to CRISPR-associated protein 9 (Cas9) and the BmOTP-Cas9 ribonucleoprotein complex was injected into female pupae, heritable editing of the offspring was achieved in the silkworms. Compared with embryo microinjection, individual injection is more convenient and eliminates the challenge of injecting extremely small embryos. Our results will significantly facilitate the genetic manipulation of other lepidopteran insects, which is essential for advancing lepidopteran pest control. |
| first_indexed | 2024-03-08T20:40:10Z |
| format | Article |
| id | doaj.art-c12f4743b93d47c397a513aa6731f94e |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-08T20:40:10Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-c12f4743b93d47c397a513aa6731f94e2023-12-22T14:16:16ZengMDPI AGInsects2075-44502023-12-01141293210.3390/insects14120932Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in <i>Bombyx mori</i>Bin Yu0Sichen Dong1Xiaoyu Jiang2Liang Qiao3Jie Chen4Tian Li5Guoqing Pan6Zeyang Zhou7Chunfeng Li8State Key Laboratory of Resource Insects, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Resource Insects, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Resource Insects, Southwest University, Chongqing 400715, ChinaCollege of Life Sciences, Chongqing Normal University, Chongqing 401331, ChinaState Key Laboratory of Resource Insects, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Resource Insects, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Resource Insects, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Resource Insects, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Resource Insects, Southwest University, Chongqing 400715, ChinaLepidoptera is one of the most speciose insect orders, causing enormous damage to agricultural and forest crops. Although genome editing has been achieved in a few Lepidoptera for insect controls, most techniques are still limited. Here, by injecting female pupae of the Lepidoptera model species, <i>Bombyx mori</i>, gene editing was established using the Receptor-Mediated Ovary Transduction of Cargo (ReMOT) control technique. We identified a <i>B. mori</i> oocytes-targeting peptide ligand (BmOTP, a 29 aa of vitellogenin N-terminal of silkworms) with a highly conserved sequence in lepidopteran insects that could efficiently deliver mCherry into oocytes. When BmOTP was fused to CRISPR-associated protein 9 (Cas9) and the BmOTP-Cas9 ribonucleoprotein complex was injected into female pupae, heritable editing of the offspring was achieved in the silkworms. Compared with embryo microinjection, individual injection is more convenient and eliminates the challenge of injecting extremely small embryos. Our results will significantly facilitate the genetic manipulation of other lepidopteran insects, which is essential for advancing lepidopteran pest control.https://www.mdpi.com/2075-4450/14/12/932ReMOT control<i>Bombyx mori</i>lepidopteran insectsCRISPR/Cas9gene editing |
| spellingShingle | Bin Yu Sichen Dong Xiaoyu Jiang Liang Qiao Jie Chen Tian Li Guoqing Pan Zeyang Zhou Chunfeng Li Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in <i>Bombyx mori</i> Insects ReMOT control <i>Bombyx mori</i> lepidopteran insects CRISPR/Cas9 gene editing |
| title | Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in <i>Bombyx mori</i> |
| title_full | Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in <i>Bombyx mori</i> |
| title_fullStr | Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in <i>Bombyx mori</i> |
| title_full_unstemmed | Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in <i>Bombyx mori</i> |
| title_short | Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in <i>Bombyx mori</i> |
| title_sort | cas9 mediated gene editing using receptor mediated ovary transduction of cargo remot control in i bombyx mori i |
| topic | ReMOT control <i>Bombyx mori</i> lepidopteran insects CRISPR/Cas9 gene editing |
| url | https://www.mdpi.com/2075-4450/14/12/932 |
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