Comparison of proteomics profiles of Campylobacter jejuni strain Bf under microaerobic and aerobic conditions
Campylobacter jejuni accounts for one of the leading causes of foodborne bacterial enteritis in humans. Despite being considered an obligate microaerobic microorganism, C. jejuni is regularly exposed to oxidative stress. However, its adaptive strategies to survive the atmospheric oxygen level during...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-10-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01596/full |
| _version_ | 1818612072260304896 |
|---|---|
| author | Ramila Christiane Rodrigues Nabila Haddad Didier Chevret Jean-Michel Cappelier Odile Tresse |
| author_facet | Ramila Christiane Rodrigues Nabila Haddad Didier Chevret Jean-Michel Cappelier Odile Tresse |
| author_sort | Ramila Christiane Rodrigues |
| collection | DOAJ |
| description | Campylobacter jejuni accounts for one of the leading causes of foodborne bacterial enteritis in humans. Despite being considered an obligate microaerobic microorganism, C. jejuni is regularly exposed to oxidative stress. However, its adaptive strategies to survive the atmospheric oxygen level during transmission to humans remain unclear. Recently, the atypical clinical C. jejuni Bf strain was shown by its unexpected ability to grow under ambient atmosphere. Here, we aimed to understand better the biological mechanisms underlying its atypical aerotolerance trait using two-dimensional protein electrophoresis, gene expression and enzymatic activities. Forty-seven proteins were identified with a significantly different abundance between cultivation under microaerobic and aerobic conditions. The over-expressed proteins in aerobiosis belonged mainly to the oxidative stress response, modulation of the main enzymes of the tricarboxylic acid cycle, iron uptake and regulation and amino acid uptake when compared to microaerobic conditions. The higher abundance of proteins related to oxidative stress was correlated to dramatically higher transcript levels of the corresponding encoding genes in aerobic conditions compared to microaerobic conditions. In addition, a higher catalase-equivalent activity in strain Bf was observed. Despite the restricted catabolic capacities of C. jejuni, this study reveals that strain Bf is equipped to withstand oxidative stress. This ability could contribute to emergence and persistence of particular strains of C. jejuni throughout food processing or macrophage attack during human infection. |
| first_indexed | 2024-12-16T15:40:24Z |
| format | Article |
| id | doaj.art-c135267d6ebd44178b610120fb0eb508 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T15:40:24Z |
| publishDate | 2016-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c135267d6ebd44178b610120fb0eb5082022-12-21T22:26:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-10-01710.3389/fmicb.2016.01596191866Comparison of proteomics profiles of Campylobacter jejuni strain Bf under microaerobic and aerobic conditionsRamila Christiane Rodrigues0Nabila Haddad1Didier Chevret2Jean-Michel Cappelier3Odile Tresse4French National Institute for Agricultural Research/Nantes-Atlantic National College of Veterinary Medicine, Food Science and EngineeringFrench National Institute for Agricultural Research/Nantes-Atlantic National College of Veterinary Medicine, Food Science and EngineeringFrench National Institute for Agriculral ResearchFrench National Institute for Agricultural Research/Nantes-Atlantic National College of Veterinary Medicine, Food Science and EngineeringFrench National Institute for Agricultural Research/Nantes-Atlantic National College of Veterinary Medicine, Food Science and EngineeringCampylobacter jejuni accounts for one of the leading causes of foodborne bacterial enteritis in humans. Despite being considered an obligate microaerobic microorganism, C. jejuni is regularly exposed to oxidative stress. However, its adaptive strategies to survive the atmospheric oxygen level during transmission to humans remain unclear. Recently, the atypical clinical C. jejuni Bf strain was shown by its unexpected ability to grow under ambient atmosphere. Here, we aimed to understand better the biological mechanisms underlying its atypical aerotolerance trait using two-dimensional protein electrophoresis, gene expression and enzymatic activities. Forty-seven proteins were identified with a significantly different abundance between cultivation under microaerobic and aerobic conditions. The over-expressed proteins in aerobiosis belonged mainly to the oxidative stress response, modulation of the main enzymes of the tricarboxylic acid cycle, iron uptake and regulation and amino acid uptake when compared to microaerobic conditions. The higher abundance of proteins related to oxidative stress was correlated to dramatically higher transcript levels of the corresponding encoding genes in aerobic conditions compared to microaerobic conditions. In addition, a higher catalase-equivalent activity in strain Bf was observed. Despite the restricted catabolic capacities of C. jejuni, this study reveals that strain Bf is equipped to withstand oxidative stress. This ability could contribute to emergence and persistence of particular strains of C. jejuni throughout food processing or macrophage attack during human infection.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01596/fullCampylobacter jejuniOxidative StressProteomicsfoodborne pathogenAerotolerance |
| spellingShingle | Ramila Christiane Rodrigues Nabila Haddad Didier Chevret Jean-Michel Cappelier Odile Tresse Comparison of proteomics profiles of Campylobacter jejuni strain Bf under microaerobic and aerobic conditions Frontiers in Microbiology Campylobacter jejuni Oxidative Stress Proteomics foodborne pathogen Aerotolerance |
| title | Comparison of proteomics profiles of Campylobacter jejuni strain Bf under microaerobic and aerobic conditions |
| title_full | Comparison of proteomics profiles of Campylobacter jejuni strain Bf under microaerobic and aerobic conditions |
| title_fullStr | Comparison of proteomics profiles of Campylobacter jejuni strain Bf under microaerobic and aerobic conditions |
| title_full_unstemmed | Comparison of proteomics profiles of Campylobacter jejuni strain Bf under microaerobic and aerobic conditions |
| title_short | Comparison of proteomics profiles of Campylobacter jejuni strain Bf under microaerobic and aerobic conditions |
| title_sort | comparison of proteomics profiles of campylobacter jejuni strain bf under microaerobic and aerobic conditions |
| topic | Campylobacter jejuni Oxidative Stress Proteomics foodborne pathogen Aerotolerance |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01596/full |
| work_keys_str_mv | AT ramilachristianerodrigues comparisonofproteomicsprofilesofcampylobacterjejunistrainbfundermicroaerobicandaerobicconditions AT nabilahaddad comparisonofproteomicsprofilesofcampylobacterjejunistrainbfundermicroaerobicandaerobicconditions AT didierchevret comparisonofproteomicsprofilesofcampylobacterjejunistrainbfundermicroaerobicandaerobicconditions AT jeanmichelcappelier comparisonofproteomicsprofilesofcampylobacterjejunistrainbfundermicroaerobicandaerobicconditions AT odiletresse comparisonofproteomicsprofilesofcampylobacterjejunistrainbfundermicroaerobicandaerobicconditions |