Cytochrome P450 <i>BsCYP99A44</i> and <i>BsCYP704A177</i> Confer Metabolic Resistance to ALS Herbicides in <i>Beckmannia syzigachne</i>
<i>Beckmannia syzigachne</i> is a noxious grassy weed that infests wheat fields in China. Previously, we identified that mesosulfuron-methyl resistance in a <i>B. syzigachne</i> population (R, SD04) was conferred by non-target resistance, such as cytochrome P450 mixed-functio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/20/12175 |
| Summary: | <i>Beckmannia syzigachne</i> is a noxious grassy weed that infests wheat fields in China. Previously, we identified that mesosulfuron-methyl resistance in a <i>B. syzigachne</i> population (R, SD04) was conferred by non-target resistance, such as cytochrome P450 mixed-function oxidases (P450s)-based metabolism. RNA sequencing and real-time PCR (qRT-PCR) were used to discover potential P450s-resistant-related genes. Five cytochrome P450s (<i>CYP704A177</i>, <i>CYP96B84</i>, <i>CYP71D7</i>, <i>CYP93A1,</i> and <i>CYP99A44</i>) were found to be highly expressed in R plants. In this study, <i>CYP99A44</i> and <i>CYP704A177</i> were cloned from <i>B. syzigachne</i> and transferred into <i>Arabidopsis thaliana</i> to test the sensitivity of <i>Arabidopsis</i> with and without P450s genes to mesosulfuron-methyl and other acetolactate synthase (ALS)-inhibiting herbicides. Transgenic <i>Arabidopsis</i> overexpressing <i>CYP99A44</i> became resistant to the sulfonylurea herbicide mesosulfuron-methyl, but showed no resistance to pyroxsulam, imazethapyr, flucarbazone, and bispyribac-sodium. Notably, those overexpressing <i>CYP704A177</i> showed resistance to pyroxsulam and bispyribac-sodium, but not to mesosulfuron-methyl, imazethapyr, and flucarbazone. These results indicated that <i>B. syzigachne</i> and transgenic <i>Arabidopsis</i> displayed different cross-resistance patterns to ALS-inhibiting herbicides. Subcellular localization revealed that <i>CYP99A44</i> and <i>CYP704A177</i> protein were located in the endoplasmic reticulum. Furthermore, these results clearly indicated that <i>CYP99A44</i>-mediated mesosulfuron-methyl resistance in <i>B. syzigachne</i> and <i>CYP704A177</i> may be involved in <i>B. syzigachne</i> cross-resistance to pyroxsulam and bispyribac-sodium. |
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| ISSN: | 1661-6596 1422-0067 |