Cytochrome P450 <i>BsCYP99A44</i> and <i>BsCYP704A177</i> Confer Metabolic Resistance to ALS Herbicides in <i>Beckmannia syzigachne</i>
<i>Beckmannia syzigachne</i> is a noxious grassy weed that infests wheat fields in China. Previously, we identified that mesosulfuron-methyl resistance in a <i>B. syzigachne</i> population (R, SD04) was conferred by non-target resistance, such as cytochrome P450 mixed-functio...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12175 |
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| author | Shuang Bai Mengjie Yin Qinghao Lyu Bo Jiang Lingxu Li |
| author_facet | Shuang Bai Mengjie Yin Qinghao Lyu Bo Jiang Lingxu Li |
| author_sort | Shuang Bai |
| collection | DOAJ |
| description | <i>Beckmannia syzigachne</i> is a noxious grassy weed that infests wheat fields in China. Previously, we identified that mesosulfuron-methyl resistance in a <i>B. syzigachne</i> population (R, SD04) was conferred by non-target resistance, such as cytochrome P450 mixed-function oxidases (P450s)-based metabolism. RNA sequencing and real-time PCR (qRT-PCR) were used to discover potential P450s-resistant-related genes. Five cytochrome P450s (<i>CYP704A177</i>, <i>CYP96B84</i>, <i>CYP71D7</i>, <i>CYP93A1,</i> and <i>CYP99A44</i>) were found to be highly expressed in R plants. In this study, <i>CYP99A44</i> and <i>CYP704A177</i> were cloned from <i>B. syzigachne</i> and transferred into <i>Arabidopsis thaliana</i> to test the sensitivity of <i>Arabidopsis</i> with and without P450s genes to mesosulfuron-methyl and other acetolactate synthase (ALS)-inhibiting herbicides. Transgenic <i>Arabidopsis</i> overexpressing <i>CYP99A44</i> became resistant to the sulfonylurea herbicide mesosulfuron-methyl, but showed no resistance to pyroxsulam, imazethapyr, flucarbazone, and bispyribac-sodium. Notably, those overexpressing <i>CYP704A177</i> showed resistance to pyroxsulam and bispyribac-sodium, but not to mesosulfuron-methyl, imazethapyr, and flucarbazone. These results indicated that <i>B. syzigachne</i> and transgenic <i>Arabidopsis</i> displayed different cross-resistance patterns to ALS-inhibiting herbicides. Subcellular localization revealed that <i>CYP99A44</i> and <i>CYP704A177</i> protein were located in the endoplasmic reticulum. Furthermore, these results clearly indicated that <i>CYP99A44</i>-mediated mesosulfuron-methyl resistance in <i>B. syzigachne</i> and <i>CYP704A177</i> may be involved in <i>B. syzigachne</i> cross-resistance to pyroxsulam and bispyribac-sodium. |
| first_indexed | 2024-03-09T09:47:12Z |
| format | Article |
| id | doaj.art-c135457783574688b0dae5f6d05df54c |
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| language | English |
| last_indexed | 2024-03-09T09:47:12Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-c135457783574688b0dae5f6d05df54c2023-12-02T00:31:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201217510.3390/ijms232012175Cytochrome P450 <i>BsCYP99A44</i> and <i>BsCYP704A177</i> Confer Metabolic Resistance to ALS Herbicides in <i>Beckmannia syzigachne</i>Shuang Bai0Mengjie Yin1Qinghao Lyu2Bo Jiang3Lingxu Li4College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China<i>Beckmannia syzigachne</i> is a noxious grassy weed that infests wheat fields in China. Previously, we identified that mesosulfuron-methyl resistance in a <i>B. syzigachne</i> population (R, SD04) was conferred by non-target resistance, such as cytochrome P450 mixed-function oxidases (P450s)-based metabolism. RNA sequencing and real-time PCR (qRT-PCR) were used to discover potential P450s-resistant-related genes. Five cytochrome P450s (<i>CYP704A177</i>, <i>CYP96B84</i>, <i>CYP71D7</i>, <i>CYP93A1,</i> and <i>CYP99A44</i>) were found to be highly expressed in R plants. In this study, <i>CYP99A44</i> and <i>CYP704A177</i> were cloned from <i>B. syzigachne</i> and transferred into <i>Arabidopsis thaliana</i> to test the sensitivity of <i>Arabidopsis</i> with and without P450s genes to mesosulfuron-methyl and other acetolactate synthase (ALS)-inhibiting herbicides. Transgenic <i>Arabidopsis</i> overexpressing <i>CYP99A44</i> became resistant to the sulfonylurea herbicide mesosulfuron-methyl, but showed no resistance to pyroxsulam, imazethapyr, flucarbazone, and bispyribac-sodium. Notably, those overexpressing <i>CYP704A177</i> showed resistance to pyroxsulam and bispyribac-sodium, but not to mesosulfuron-methyl, imazethapyr, and flucarbazone. These results indicated that <i>B. syzigachne</i> and transgenic <i>Arabidopsis</i> displayed different cross-resistance patterns to ALS-inhibiting herbicides. Subcellular localization revealed that <i>CYP99A44</i> and <i>CYP704A177</i> protein were located in the endoplasmic reticulum. Furthermore, these results clearly indicated that <i>CYP99A44</i>-mediated mesosulfuron-methyl resistance in <i>B. syzigachne</i> and <i>CYP704A177</i> may be involved in <i>B. syzigachne</i> cross-resistance to pyroxsulam and bispyribac-sodium.https://www.mdpi.com/1422-0067/23/20/12175<i>Beckmannia syzigachne</i>cytochrome P450smesosulfuron-methylnon-target-site resistancemechanism |
| spellingShingle | Shuang Bai Mengjie Yin Qinghao Lyu Bo Jiang Lingxu Li Cytochrome P450 <i>BsCYP99A44</i> and <i>BsCYP704A177</i> Confer Metabolic Resistance to ALS Herbicides in <i>Beckmannia syzigachne</i> International Journal of Molecular Sciences <i>Beckmannia syzigachne</i> cytochrome P450s mesosulfuron-methyl non-target-site resistance mechanism |
| title | Cytochrome P450 <i>BsCYP99A44</i> and <i>BsCYP704A177</i> Confer Metabolic Resistance to ALS Herbicides in <i>Beckmannia syzigachne</i> |
| title_full | Cytochrome P450 <i>BsCYP99A44</i> and <i>BsCYP704A177</i> Confer Metabolic Resistance to ALS Herbicides in <i>Beckmannia syzigachne</i> |
| title_fullStr | Cytochrome P450 <i>BsCYP99A44</i> and <i>BsCYP704A177</i> Confer Metabolic Resistance to ALS Herbicides in <i>Beckmannia syzigachne</i> |
| title_full_unstemmed | Cytochrome P450 <i>BsCYP99A44</i> and <i>BsCYP704A177</i> Confer Metabolic Resistance to ALS Herbicides in <i>Beckmannia syzigachne</i> |
| title_short | Cytochrome P450 <i>BsCYP99A44</i> and <i>BsCYP704A177</i> Confer Metabolic Resistance to ALS Herbicides in <i>Beckmannia syzigachne</i> |
| title_sort | cytochrome p450 i bscyp99a44 i and i bscyp704a177 i confer metabolic resistance to als herbicides in i beckmannia syzigachne i |
| topic | <i>Beckmannia syzigachne</i> cytochrome P450s mesosulfuron-methyl non-target-site resistance mechanism |
| url | https://www.mdpi.com/1422-0067/23/20/12175 |
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