Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering
Background: In end-stage chronic liver disease, transplantation represents the only curative option. However, the shortage of donors results in the death of many patients. To overcome this gap, it is mandatory to develop new therapeutic options. In the present study, we decellularised pig livers and...
| Main Authors: | , , , , , , , , , , , , , , , |
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MDPI AG
2022-11-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/11/2817 |
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| author | Stefania Croce Lorenzo Cobianchi Tamara Zoro Francesca Dal Mas Antonia Icaro Cornaglia Elisa Lenta Gloria Acquafredda Annalisa De Silvestri Maria Antonietta Avanzini Livia Visai Szandra Brambilla Giovanna Bruni Giulia Di Gravina Andrea Pietrabissa Luca Ansaloni Andrea Peloso |
| author_facet | Stefania Croce Lorenzo Cobianchi Tamara Zoro Francesca Dal Mas Antonia Icaro Cornaglia Elisa Lenta Gloria Acquafredda Annalisa De Silvestri Maria Antonietta Avanzini Livia Visai Szandra Brambilla Giovanna Bruni Giulia Di Gravina Andrea Pietrabissa Luca Ansaloni Andrea Peloso |
| author_sort | Stefania Croce |
| collection | DOAJ |
| description | Background: In end-stage chronic liver disease, transplantation represents the only curative option. However, the shortage of donors results in the death of many patients. To overcome this gap, it is mandatory to develop new therapeutic options. In the present study, we decellularised pig livers and reseeded them with allogeneic porcine mesenchymal stromal cells (pMSCs) to understand whether extracellular matrix (ECM) can influence and/or promote differentiation into hepatocyte-like cells (HLCs). Methods: After decellularisation with SDS, the integrity of ECM-scaffolds was examined by histological staining, immunofluorescence and scanning electron microscope. DNA quantification was used to assess decellularisation. pMSCs were plated on scaffolds by static seeding and maintained in in vitro culture for 21 days. At 3, 7, 14 and 21 days, seeded ECM scaffolds were evaluated for cellular adhesion and growth. Moreover, the expression of specific hepatic genes was performed by RT-PCR. Results: The applied decellularisation/recellularisation protocol was effective. The number of seeded pMSCs increased over the culture time points. Gene expression analysis of seeded pMSCs displayed a weak induction due to ECM towards HLCs. Conclusions: These results suggest that ECM may address pMSCs to differentiate in hepatocyte-like cells. However, only contact with liver-ECM is not enough to induce complete differentiation. |
| first_indexed | 2024-03-09T19:15:30Z |
| format | Article |
| id | doaj.art-c13692720d514f8f9ed025ab0c95ad23 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T19:15:30Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-c13692720d514f8f9ed025ab0c95ad232023-11-24T03:51:10ZengMDPI AGBiomedicines2227-90592022-11-011011281710.3390/biomedicines10112817Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue EngineeringStefania Croce0Lorenzo Cobianchi1Tamara Zoro2Francesca Dal Mas3Antonia Icaro Cornaglia4Elisa Lenta5Gloria Acquafredda6Annalisa De Silvestri7Maria Antonietta Avanzini8Livia Visai9Szandra Brambilla10Giovanna Bruni11Giulia Di Gravina12Andrea Pietrabissa13Luca Ansaloni14Andrea Peloso15Department of Clinical, Surgical, Diagnostic & Pediatric Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of Clinical, Surgical, Diagnostic & Pediatric Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of General Surgery, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyDepartment of Management, Ca’ Foscari University of Venice, 30100 Venice, ItalyHistology & Embryology Unit, Department of Public Health, Experimental Medicine & Forensic, University of Pavia, 27100 Pavia, ItalyImmunology and Transplantation Laboratory, Cell Factory, Pediatric Hematology Oncology, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyImmunology and Transplantation Laboratory, Cell Factory, Pediatric Hematology Oncology, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyBiometry & Clinical Epidemiology, Scientific Direction, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyImmunology and Transplantation Laboratory, Cell Factory, Pediatric Hematology Oncology, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyCenter for Health Technologies (CHT), Department of Molecular Medicine, INSTM UdR of Pavia, University of Pavia, Viale Taramelli 3/b, 27100 Pavia, ItalyCenter for Health Technologies (CHT), Department of Molecular Medicine, INSTM UdR of Pavia, University of Pavia, Viale Taramelli 3/b, 27100 Pavia, ItalyCSGI Department of Physical Chemistry M Rolla, 27100 Pavia, ItalyDepartment of Industrial and Information Engineering, University of Pavia, 27100 Pavia, ItalyDepartment of Clinical, Surgical, Diagnostic & Pediatric Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of General Surgery, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyHepatology and Transplantation Laboratory, Department of Surgery, Faculty of Medicine, University of Geneva, 1205 Geneva, SwitzerlandBackground: In end-stage chronic liver disease, transplantation represents the only curative option. However, the shortage of donors results in the death of many patients. To overcome this gap, it is mandatory to develop new therapeutic options. In the present study, we decellularised pig livers and reseeded them with allogeneic porcine mesenchymal stromal cells (pMSCs) to understand whether extracellular matrix (ECM) can influence and/or promote differentiation into hepatocyte-like cells (HLCs). Methods: After decellularisation with SDS, the integrity of ECM-scaffolds was examined by histological staining, immunofluorescence and scanning electron microscope. DNA quantification was used to assess decellularisation. pMSCs were plated on scaffolds by static seeding and maintained in in vitro culture for 21 days. At 3, 7, 14 and 21 days, seeded ECM scaffolds were evaluated for cellular adhesion and growth. Moreover, the expression of specific hepatic genes was performed by RT-PCR. Results: The applied decellularisation/recellularisation protocol was effective. The number of seeded pMSCs increased over the culture time points. Gene expression analysis of seeded pMSCs displayed a weak induction due to ECM towards HLCs. Conclusions: These results suggest that ECM may address pMSCs to differentiate in hepatocyte-like cells. However, only contact with liver-ECM is not enough to induce complete differentiation.https://www.mdpi.com/2227-9059/10/11/2817mesenchymal stromal cellsbioscaffolddecellularisationextracellular matrixrecellularisationtissue engineering |
| spellingShingle | Stefania Croce Lorenzo Cobianchi Tamara Zoro Francesca Dal Mas Antonia Icaro Cornaglia Elisa Lenta Gloria Acquafredda Annalisa De Silvestri Maria Antonietta Avanzini Livia Visai Szandra Brambilla Giovanna Bruni Giulia Di Gravina Andrea Pietrabissa Luca Ansaloni Andrea Peloso Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering Biomedicines mesenchymal stromal cells bioscaffold decellularisation extracellular matrix recellularisation tissue engineering |
| title | Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering |
| title_full | Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering |
| title_fullStr | Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering |
| title_full_unstemmed | Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering |
| title_short | Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering |
| title_sort | mesenchymal stromal cell on liver decellularised extracellular matrix for tissue engineering |
| topic | mesenchymal stromal cells bioscaffold decellularisation extracellular matrix recellularisation tissue engineering |
| url | https://www.mdpi.com/2227-9059/10/11/2817 |
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