Adenine sulfate effect on shoot formation of <i>Phaseolus vulgaris</i> L. cultivar `CIAP7247F' via indirect organogenesis
This study aimed to determine the effect of adenine sulfate in plant regeneration from callus of Phaseolus vulgaris L. cultivar `CIAP 7247F'. To this, it was added two concentrations (20 and 40 mg l-1) adenine sulfate to the regeneration culture medium and it was included a control to form a to...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2015-04-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/17 |
| _version_ | 1818854914344878080 |
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| author | Novisel Veitía Lourdes R García Amanda Martirena Ramírez Idalmis Bermúdez Caraballoso Raúl Collado Damaris Torres Carlos Romero |
| author_facet | Novisel Veitía Lourdes R García Amanda Martirena Ramírez Idalmis Bermúdez Caraballoso Raúl Collado Damaris Torres Carlos Romero |
| author_sort | Novisel Veitía |
| collection | DOAJ |
| description | This study aimed to determine the effect of adenine sulfate in plant regeneration from callus of Phaseolus vulgaris L. cultivar `CIAP 7247F'. To this, it was added two concentrations (20 and 40 mg l-1) adenine sulfate to the regeneration culture medium and it was included a control to form a total of three treatments. The number of formed shoots was quantified by treatment in the regeneration culture medium and the height of shoot was measured in elongation medium. It was determined that the use 40 mg l-1 adenine sulfate in the culture medium increased the number of shoots per callus. In the present study it was demonstrated that the addition of adenine sulfate stimulated the formation and elongation of shoots obtaining from organogenic callus in common bean cultivar `CIAP 7247F'.
Key words: callus, common bean, in vitro regeneration, tissue culture |
| first_indexed | 2024-12-19T08:00:17Z |
| format | Article |
| id | doaj.art-c143e4b73dcd41bdac1e865b3c1973ae |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-19T08:00:17Z |
| publishDate | 2015-04-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-c143e4b73dcd41bdac1e865b3c1973ae2022-12-21T20:29:53ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472015-04-0115215Adenine sulfate effect on shoot formation of <i>Phaseolus vulgaris</i> L. cultivar `CIAP7247F' via indirect organogenesisNovisel Veitía0Lourdes R García1Amanda Martirena Ramírez2Idalmis Bermúdez Caraballoso3Raúl Collado4Damaris Torres5Carlos Romero6Instituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las VillasInstituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las VillasInstituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las VillasInstituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las VillasInstituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las VillasInstituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las VillasInstituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las VillasThis study aimed to determine the effect of adenine sulfate in plant regeneration from callus of Phaseolus vulgaris L. cultivar `CIAP 7247F'. To this, it was added two concentrations (20 and 40 mg l-1) adenine sulfate to the regeneration culture medium and it was included a control to form a total of three treatments. The number of formed shoots was quantified by treatment in the regeneration culture medium and the height of shoot was measured in elongation medium. It was determined that the use 40 mg l-1 adenine sulfate in the culture medium increased the number of shoots per callus. In the present study it was demonstrated that the addition of adenine sulfate stimulated the formation and elongation of shoots obtaining from organogenic callus in common bean cultivar `CIAP 7247F'. Key words: callus, common bean, in vitro regeneration, tissue culturehttps://revista.ibp.co.cu/index.php/BV/article/view/17 |
| spellingShingle | Novisel Veitía Lourdes R García Amanda Martirena Ramírez Idalmis Bermúdez Caraballoso Raúl Collado Damaris Torres Carlos Romero Adenine sulfate effect on shoot formation of <i>Phaseolus vulgaris</i> L. cultivar `CIAP7247F' via indirect organogenesis Biotecnología Vegetal |
| title | Adenine sulfate effect on shoot formation of <i>Phaseolus vulgaris</i> L. cultivar `CIAP7247F' via indirect organogenesis |
| title_full | Adenine sulfate effect on shoot formation of <i>Phaseolus vulgaris</i> L. cultivar `CIAP7247F' via indirect organogenesis |
| title_fullStr | Adenine sulfate effect on shoot formation of <i>Phaseolus vulgaris</i> L. cultivar `CIAP7247F' via indirect organogenesis |
| title_full_unstemmed | Adenine sulfate effect on shoot formation of <i>Phaseolus vulgaris</i> L. cultivar `CIAP7247F' via indirect organogenesis |
| title_short | Adenine sulfate effect on shoot formation of <i>Phaseolus vulgaris</i> L. cultivar `CIAP7247F' via indirect organogenesis |
| title_sort | adenine sulfate effect on shoot formation of i phaseolus vulgaris i l cultivar ciap7247f via indirect organogenesis |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/17 |
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