Digital NFATc2 activation per cell transforms graded T cell receptor activation into an all-or-none IL-2 expression.
The expression of interleukin-2 (IL-2) is a key event in T helper (Th) lymphocyte activation, controlling both, the expansion and differentiation of effector Th cells as well as the activation of regulatory T cells. We demonstrate that the strength of TCR stimulation is translated into the frequency...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2007-09-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0000935 |
| _version_ | 1819108545778417664 |
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| author | Miriam Podtschaske Uwe Benary Sandra Zwinger Thomas Höfer Andreas Radbruch Ria Baumgrass |
| author_facet | Miriam Podtschaske Uwe Benary Sandra Zwinger Thomas Höfer Andreas Radbruch Ria Baumgrass |
| author_sort | Miriam Podtschaske |
| collection | DOAJ |
| description | The expression of interleukin-2 (IL-2) is a key event in T helper (Th) lymphocyte activation, controlling both, the expansion and differentiation of effector Th cells as well as the activation of regulatory T cells. We demonstrate that the strength of TCR stimulation is translated into the frequency of memory Th cells expressing IL-2 but not into the amount of IL-2 per cell. This molecular switch decision for IL-2 expression per cell is located downstream of the cytosolic Ca2+ level. Here we show that in a single activated Th cell, NFATc2 activation is digital but NF-kappaB activation is graded after graded T cell receptor (TCR) signaling. Subsequently, NFATc2 translocates into the nucleus in an all-or-none fashion per cell, transforming the strength of TCR-stimulation into the number of nuclei positive for NFATc2 and IL-2 transcription. Thus, the described NFATc2 switch regulates the number of Th cells actively participating in an immune response. |
| first_indexed | 2024-12-22T03:11:38Z |
| format | Article |
| id | doaj.art-c146b7498b194d678b9ec4d15b08291e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T03:11:38Z |
| publishDate | 2007-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c146b7498b194d678b9ec4d15b08291e2022-12-21T18:40:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032007-09-0129e93510.1371/journal.pone.0000935Digital NFATc2 activation per cell transforms graded T cell receptor activation into an all-or-none IL-2 expression.Miriam PodtschaskeUwe BenarySandra ZwingerThomas HöferAndreas RadbruchRia BaumgrassThe expression of interleukin-2 (IL-2) is a key event in T helper (Th) lymphocyte activation, controlling both, the expansion and differentiation of effector Th cells as well as the activation of regulatory T cells. We demonstrate that the strength of TCR stimulation is translated into the frequency of memory Th cells expressing IL-2 but not into the amount of IL-2 per cell. This molecular switch decision for IL-2 expression per cell is located downstream of the cytosolic Ca2+ level. Here we show that in a single activated Th cell, NFATc2 activation is digital but NF-kappaB activation is graded after graded T cell receptor (TCR) signaling. Subsequently, NFATc2 translocates into the nucleus in an all-or-none fashion per cell, transforming the strength of TCR-stimulation into the number of nuclei positive for NFATc2 and IL-2 transcription. Thus, the described NFATc2 switch regulates the number of Th cells actively participating in an immune response.https://doi.org/10.1371/journal.pone.0000935 |
| spellingShingle | Miriam Podtschaske Uwe Benary Sandra Zwinger Thomas Höfer Andreas Radbruch Ria Baumgrass Digital NFATc2 activation per cell transforms graded T cell receptor activation into an all-or-none IL-2 expression. PLoS ONE |
| title | Digital NFATc2 activation per cell transforms graded T cell receptor activation into an all-or-none IL-2 expression. |
| title_full | Digital NFATc2 activation per cell transforms graded T cell receptor activation into an all-or-none IL-2 expression. |
| title_fullStr | Digital NFATc2 activation per cell transforms graded T cell receptor activation into an all-or-none IL-2 expression. |
| title_full_unstemmed | Digital NFATc2 activation per cell transforms graded T cell receptor activation into an all-or-none IL-2 expression. |
| title_short | Digital NFATc2 activation per cell transforms graded T cell receptor activation into an all-or-none IL-2 expression. |
| title_sort | digital nfatc2 activation per cell transforms graded t cell receptor activation into an all or none il 2 expression |
| url | https://doi.org/10.1371/journal.pone.0000935 |
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