Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet form
Abstract This study introduced an automated long‐term fermentation process for fungals grown in pellet form. The goal was to reduce the overgrowth of bioreactor internals and sensors while better rheological properties in the fermentation broth, such as oxygen transfer and mixing time, can be achiev...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2022-07-01
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| Series: | Engineering in Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/elsc.202100094 |
| _version_ | 1818097276813312000 |
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| author | Winda Soerjawinata Isabelle Kockler Lars Wommer Robert Frank Anja Schüffler Tanja Schirmeister Roland Ulber Percy Kampeis |
| author_facet | Winda Soerjawinata Isabelle Kockler Lars Wommer Robert Frank Anja Schüffler Tanja Schirmeister Roland Ulber Percy Kampeis |
| author_sort | Winda Soerjawinata |
| collection | DOAJ |
| description | Abstract This study introduced an automated long‐term fermentation process for fungals grown in pellet form. The goal was to reduce the overgrowth of bioreactor internals and sensors while better rheological properties in the fermentation broth, such as oxygen transfer and mixing time, can be achieved. Because this could not be accomplished with continuous culture and fed‐batch fermentation, repeated‐batch fermentation was implemented with the help of additional bioreactor internals (“sporulation supports”). This should capture some biomass during fermentation. After harvesting the suspended biomass, intermediate cleaning was performed using a cleaning device. The biomass retained on the sporulation support went through the sporulation phase. The spores were subsequently used as inocula for the next batch. The reason for this approach was that the retained pellets could otherwise cause problems (e.g., overgrowth on sensors) in subsequent batches because the fungus would then show undesirable hyphal growth. Various sporulation supports were tested for sufficient biomass fixation to start the next batch. A reproducible spore concentration within the range of the requirements could be achieved by adjusting the sporulation support (design and construction material), and an intermediate cleaning adapted to this. |
| first_indexed | 2024-12-10T23:17:57Z |
| format | Article |
| id | doaj.art-c1491132795f44cc968c3eaabd04f0e3 |
| institution | Directory Open Access Journal |
| issn | 1618-0240 1618-2863 |
| language | English |
| last_indexed | 2024-12-10T23:17:57Z |
| publishDate | 2022-07-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj.art-c1491132795f44cc968c3eaabd04f0e32022-12-22T01:29:48ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632022-07-0122747448310.1002/elsc.202100094Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet formWinda Soerjawinata0Isabelle Kockler1Lars Wommer2Robert Frank3Anja Schüffler4Tanja Schirmeister5Roland Ulber6Percy Kampeis7Institute for Biotechnical Process Design Trier University of Applied Sciences, Environmental Campus Birkenfeld Hoppstädten‐Weiersbach GermanyInstitute for Biotechnical Process Design Trier University of Applied Sciences, Environmental Campus Birkenfeld Hoppstädten‐Weiersbach GermanyInstitute for Biotechnical Process Design Trier University of Applied Sciences, Environmental Campus Birkenfeld Hoppstädten‐Weiersbach GermanyInstitute for Biotechnical Process Design Trier University of Applied Sciences, Environmental Campus Birkenfeld Hoppstädten‐Weiersbach GermanyInstitut für Biotechnologie und Wirkstoff‐Forschung gGmbH (IBWF) Mainz GermanyInstitute of Pharmaceutical and Biomedical Sciences Johannes Gutenberg University of Mainz Mainz GermanyInstitute of Bioprocess Engineering Technical University Kaiserslautern Kaiserslautern GermanyInstitute for Biotechnical Process Design Trier University of Applied Sciences, Environmental Campus Birkenfeld Hoppstädten‐Weiersbach GermanyAbstract This study introduced an automated long‐term fermentation process for fungals grown in pellet form. The goal was to reduce the overgrowth of bioreactor internals and sensors while better rheological properties in the fermentation broth, such as oxygen transfer and mixing time, can be achieved. Because this could not be accomplished with continuous culture and fed‐batch fermentation, repeated‐batch fermentation was implemented with the help of additional bioreactor internals (“sporulation supports”). This should capture some biomass during fermentation. After harvesting the suspended biomass, intermediate cleaning was performed using a cleaning device. The biomass retained on the sporulation support went through the sporulation phase. The spores were subsequently used as inocula for the next batch. The reason for this approach was that the retained pellets could otherwise cause problems (e.g., overgrowth on sensors) in subsequent batches because the fungus would then show undesirable hyphal growth. Various sporulation supports were tested for sufficient biomass fixation to start the next batch. A reproducible spore concentration within the range of the requirements could be achieved by adjusting the sporulation support (design and construction material), and an intermediate cleaning adapted to this.https://doi.org/10.1002/elsc.202100094bioreactor internalsfungal growthfungal pelletsrepeated‐batch fermentationsporulation conditions |
| spellingShingle | Winda Soerjawinata Isabelle Kockler Lars Wommer Robert Frank Anja Schüffler Tanja Schirmeister Roland Ulber Percy Kampeis Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet form Engineering in Life Sciences bioreactor internals fungal growth fungal pellets repeated‐batch fermentation sporulation conditions |
| title | Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet form |
| title_full | Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet form |
| title_fullStr | Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet form |
| title_full_unstemmed | Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet form |
| title_short | Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet form |
| title_sort | novel bioreactor internals for the cultivation of spore forming fungi in pellet form |
| topic | bioreactor internals fungal growth fungal pellets repeated‐batch fermentation sporulation conditions |
| url | https://doi.org/10.1002/elsc.202100094 |
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