Identification of Novel Conotoxin Precursors from the Cone Snail <i>Conus spurius</i> by High-Throughput RNA Sequencing
Marine gastropods of the genus <i>Conus</i>, comprising more than 800 species, have the characteristic of injecting worms and other prey with venom. These conopeptide toxins, highly diverse in structure and action, are highly potent and specific for their molecular targets (ion channels,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/10/547 |
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| author | Roberto Zamora-Bustillos Mario Alberto Martínez-Núñez Manuel B. Aguilar Reyna Cristina Collí-Dula Diego Alfredo Brito-Domínguez |
| author_facet | Roberto Zamora-Bustillos Mario Alberto Martínez-Núñez Manuel B. Aguilar Reyna Cristina Collí-Dula Diego Alfredo Brito-Domínguez |
| author_sort | Roberto Zamora-Bustillos |
| collection | DOAJ |
| description | Marine gastropods of the genus <i>Conus</i>, comprising more than 800 species, have the characteristic of injecting worms and other prey with venom. These conopeptide toxins, highly diverse in structure and action, are highly potent and specific for their molecular targets (ion channels, receptors, and transporters of the prey’s nervous system), and thus are important research tools and source for drug discovery. Next-generation sequencing technologies are speeding up the discovery of novel conopeptides in many of these species, but only limited information is available for <i>Conus spurius</i>, which inhabits sandy mud. To search for new precursor conopeptides, we analyzed the transcriptome of the venous ducts of <i>C. spurius</i> and identified 55 putative conotoxins. Seven were selected for further study and confirmed by Sanger sequencing to belong to the M-superfamily (Sr3.M01 and Sr3.M02), A-superfamily (Sr1.A01 and Sr1.A02), O-superfamily (Sr15.O01), and Con-ikot-ikot (Sr21.CII01 and Sr22.CII02). Six of these have never been reported. To our knowledge, this report is the first to use high-throughput RNA sequencing for the study of the diversity of <i>C. spurius</i> conotoxins. |
| first_indexed | 2024-03-10T06:26:18Z |
| format | Article |
| id | doaj.art-c149dca72bfc4774b85d1784874a3c19 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T06:26:18Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-c149dca72bfc4774b85d1784874a3c192023-11-22T18:55:08ZengMDPI AGMarine Drugs1660-33972021-09-01191054710.3390/md19100547Identification of Novel Conotoxin Precursors from the Cone Snail <i>Conus spurius</i> by High-Throughput RNA SequencingRoberto Zamora-Bustillos0Mario Alberto Martínez-Núñez1Manuel B. Aguilar2Reyna Cristina Collí-Dula3Diego Alfredo Brito-Domínguez4División de Estudios de Posgrado e Investigación, TecNM/Instituto Tecnológico de Conkal, Av. Tecnológico S/N, Conkal 97345, Yucatán, MexicoUnidad Multidisciplinaria de Docencia e Investigación, UMDI-SISAL, Facultad de Ciencias, Universidad Nacional Autónoma de México, Sierra Papacal-Chuburna Km 5, Mérida 97302, Yucatán, MexicoLaboratorio de Neurofarmacología Marina, Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Juriquilla 76230, Querétaro, MexicoDepartamento de Recursos del Mar, Cinvestav Unidad Mérida, Mérida 97310, Yucatán, MexicoDivisión de Estudios de Posgrado e Investigación, TecNM/Instituto Tecnológico de Conkal, Av. Tecnológico S/N, Conkal 97345, Yucatán, MexicoMarine gastropods of the genus <i>Conus</i>, comprising more than 800 species, have the characteristic of injecting worms and other prey with venom. These conopeptide toxins, highly diverse in structure and action, are highly potent and specific for their molecular targets (ion channels, receptors, and transporters of the prey’s nervous system), and thus are important research tools and source for drug discovery. Next-generation sequencing technologies are speeding up the discovery of novel conopeptides in many of these species, but only limited information is available for <i>Conus spurius</i>, which inhabits sandy mud. To search for new precursor conopeptides, we analyzed the transcriptome of the venous ducts of <i>C. spurius</i> and identified 55 putative conotoxins. Seven were selected for further study and confirmed by Sanger sequencing to belong to the M-superfamily (Sr3.M01 and Sr3.M02), A-superfamily (Sr1.A01 and Sr1.A02), O-superfamily (Sr15.O01), and Con-ikot-ikot (Sr21.CII01 and Sr22.CII02). Six of these have never been reported. To our knowledge, this report is the first to use high-throughput RNA sequencing for the study of the diversity of <i>C. spurius</i> conotoxins.https://www.mdpi.com/1660-3397/19/10/547<i>Conus spurius</i>conopeptide precursorssuperfamilytranscriptome |
| spellingShingle | Roberto Zamora-Bustillos Mario Alberto Martínez-Núñez Manuel B. Aguilar Reyna Cristina Collí-Dula Diego Alfredo Brito-Domínguez Identification of Novel Conotoxin Precursors from the Cone Snail <i>Conus spurius</i> by High-Throughput RNA Sequencing Marine Drugs <i>Conus spurius</i> conopeptide precursors superfamily transcriptome |
| title | Identification of Novel Conotoxin Precursors from the Cone Snail <i>Conus spurius</i> by High-Throughput RNA Sequencing |
| title_full | Identification of Novel Conotoxin Precursors from the Cone Snail <i>Conus spurius</i> by High-Throughput RNA Sequencing |
| title_fullStr | Identification of Novel Conotoxin Precursors from the Cone Snail <i>Conus spurius</i> by High-Throughput RNA Sequencing |
| title_full_unstemmed | Identification of Novel Conotoxin Precursors from the Cone Snail <i>Conus spurius</i> by High-Throughput RNA Sequencing |
| title_short | Identification of Novel Conotoxin Precursors from the Cone Snail <i>Conus spurius</i> by High-Throughput RNA Sequencing |
| title_sort | identification of novel conotoxin precursors from the cone snail i conus spurius i by high throughput rna sequencing |
| topic | <i>Conus spurius</i> conopeptide precursors superfamily transcriptome |
| url | https://www.mdpi.com/1660-3397/19/10/547 |
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