Co-Overexpression of <i>RIB1</i> and <i>RIB6</i> Increases Riboflavin Production in the Yeast <i>Candida famata</i>
Riboflavin or vitamin B2 is a water-soluble vitamin and a precursor of flavin coenzymes, flavin mononucleotide, and flavin adenine dinucleotide, which play a key role as enzyme cofactors in energy metabolism. <i>Candida famata</i> yeast is a promising producer of riboflavin, as it belong...
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MDPI AG
2022-03-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/4/141 |
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| author | Yana Petrovska Oleksii Lyzak Justyna Ruchala Kostyantyn Dmytruk Andriy Sibirny |
| author_facet | Yana Petrovska Oleksii Lyzak Justyna Ruchala Kostyantyn Dmytruk Andriy Sibirny |
| author_sort | Yana Petrovska |
| collection | DOAJ |
| description | Riboflavin or vitamin B2 is a water-soluble vitamin and a precursor of flavin coenzymes, flavin mononucleotide, and flavin adenine dinucleotide, which play a key role as enzyme cofactors in energy metabolism. <i>Candida famata</i> yeast is a promising producer of riboflavin, as it belongs to the group of so-called flavinogenic yeasts, capable of riboflavin oversynthesis under conditions of iron starvation. The role of the particular structural genes in the limitation of riboflavin oversynthesis is not known. To study the impact of overexpression of the structural genes of riboflavin synthesis on riboflavin production, a set of plasmids containing genes <i>RIB1</i>, <i>RIB6,</i> and <i>RIB7</i> in different combinations was constructed. The transformants of the wild-type strain of <i>C. famata</i>, as well as riboflavin overproducer, were obtained, and the synthesis of riboflavin was studied. It was found that overexpression of <i>RIB1</i> and <i>RIB6</i> genes coding for enzymes GTP cyclohydrolase II and 3,4-dihydroxy-2-butanone-4-phosphate synthase, which catalase the initial steps of riboflavin synthesis, elevated riboflavin production by 13–28% relative to the parental riboflavin-overproducing strains. |
| first_indexed | 2024-03-09T10:38:00Z |
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| id | doaj.art-c14a5eae1b994fd7a98d4fa62504f8e4 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T10:38:00Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-c14a5eae1b994fd7a98d4fa62504f8e42023-12-01T20:51:47ZengMDPI AGFermentation2311-56372022-03-018414110.3390/fermentation8040141Co-Overexpression of <i>RIB1</i> and <i>RIB6</i> Increases Riboflavin Production in the Yeast <i>Candida famata</i>Yana Petrovska0Oleksii Lyzak1Justyna Ruchala2Kostyantyn Dmytruk3Andriy Sibirny4Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St., 14/16, 79005 Lviv, UkraineLaboratory of Chromosome Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, GermanyInstitute of Biology and Biotechnology, University of Rzeszow, Zelwerowicza 4, 35-601 Rzeszow, PolandInstitute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St., 14/16, 79005 Lviv, UkraineInstitute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St., 14/16, 79005 Lviv, UkraineRiboflavin or vitamin B2 is a water-soluble vitamin and a precursor of flavin coenzymes, flavin mononucleotide, and flavin adenine dinucleotide, which play a key role as enzyme cofactors in energy metabolism. <i>Candida famata</i> yeast is a promising producer of riboflavin, as it belongs to the group of so-called flavinogenic yeasts, capable of riboflavin oversynthesis under conditions of iron starvation. The role of the particular structural genes in the limitation of riboflavin oversynthesis is not known. To study the impact of overexpression of the structural genes of riboflavin synthesis on riboflavin production, a set of plasmids containing genes <i>RIB1</i>, <i>RIB6,</i> and <i>RIB7</i> in different combinations was constructed. The transformants of the wild-type strain of <i>C. famata</i>, as well as riboflavin overproducer, were obtained, and the synthesis of riboflavin was studied. It was found that overexpression of <i>RIB1</i> and <i>RIB6</i> genes coding for enzymes GTP cyclohydrolase II and 3,4-dihydroxy-2-butanone-4-phosphate synthase, which catalase the initial steps of riboflavin synthesis, elevated riboflavin production by 13–28% relative to the parental riboflavin-overproducing strains.https://www.mdpi.com/2311-5637/8/4/141vitamin B<sub>2</sub>riboflavin<i>Candida famata</i>yeastriboflavin overproducers |
| spellingShingle | Yana Petrovska Oleksii Lyzak Justyna Ruchala Kostyantyn Dmytruk Andriy Sibirny Co-Overexpression of <i>RIB1</i> and <i>RIB6</i> Increases Riboflavin Production in the Yeast <i>Candida famata</i> Fermentation vitamin B<sub>2</sub> riboflavin <i>Candida famata</i> yeast riboflavin overproducers |
| title | Co-Overexpression of <i>RIB1</i> and <i>RIB6</i> Increases Riboflavin Production in the Yeast <i>Candida famata</i> |
| title_full | Co-Overexpression of <i>RIB1</i> and <i>RIB6</i> Increases Riboflavin Production in the Yeast <i>Candida famata</i> |
| title_fullStr | Co-Overexpression of <i>RIB1</i> and <i>RIB6</i> Increases Riboflavin Production in the Yeast <i>Candida famata</i> |
| title_full_unstemmed | Co-Overexpression of <i>RIB1</i> and <i>RIB6</i> Increases Riboflavin Production in the Yeast <i>Candida famata</i> |
| title_short | Co-Overexpression of <i>RIB1</i> and <i>RIB6</i> Increases Riboflavin Production in the Yeast <i>Candida famata</i> |
| title_sort | co overexpression of i rib1 i and i rib6 i increases riboflavin production in the yeast i candida famata i |
| topic | vitamin B<sub>2</sub> riboflavin <i>Candida famata</i> yeast riboflavin overproducers |
| url | https://www.mdpi.com/2311-5637/8/4/141 |
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