Identifikasi Trenggiling (Manis javanica) Menggunakan Penanda Cytochrome B Mitokondria DNA (IDENTIFICATION OF PANGOLIN (MANIS JAVANICA DESMAREST, 1822) USING CYTOCHROME B mtDNA MARKER)

The aim of this study was to identify the gentic profile of Malay pangolin (Manis javanica) and originpatterns of confiscated specimens.  Tissue samples of Malay pangolin were collected from several confiscatedmaterials in Tangerang, Medan, and Lampung. Wild collections tissue were also conducted in...

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Main Authors: Wirdateti ., Gono Semiadi, Yulianto .
Format: Article
Language:English
Published: Universitas Udayana 2013-12-01
Series:Jurnal Veteriner
Subjects:
Online Access:http://ojs.unud.ac.id/index.php/jvet/article/view/7682
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author Wirdateti .
Gono Semiadi
Yulianto .
author_facet Wirdateti .
Gono Semiadi
Yulianto .
author_sort Wirdateti .
collection DOAJ
description The aim of this study was to identify the gentic profile of Malay pangolin (Manis javanica) and originpatterns of confiscated specimens.  Tissue samples of Malay pangolin were collected from several confiscatedmaterials in Tangerang, Medan, and Lampung. Wild collections tissue were also conducted in Lampungand Sukabumi. The study was conducted using  conserved Cytochrome b (Cyt. B) DNA mitochondria(mtDNA). The results showed that based on nucleotide base lentgh of 420 nt, confiscated pangolin wasdistributed in three clades and two groups. Haplotype variations was high, consisted of 19 haplotypes in19 individuals (TR1-TR19). On fisrt clade (TR4,7,16,9,19) high substitution occured in adenin base, cladetwo (TR14,17,1,2,15,3,8,13) high substitution occured in guanin base and clade three  (TR5,6,10,11,12) incytosin. It was concluded that haplotipe variation of each populations  was high and for genetic distancebetween individuals was low. Mutation rates was dominated by transition  from guanine to adenine
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spelling doaj.art-c150005718ac4948bf3e83aafe0eb5162022-12-21T19:27:16ZengUniversitas UdayanaJurnal Veteriner1411-83272477-56652013-12-011446624Identifikasi Trenggiling (Manis javanica) Menggunakan Penanda Cytochrome B Mitokondria DNA (IDENTIFICATION OF PANGOLIN (MANIS JAVANICA DESMAREST, 1822) USING CYTOCHROME B mtDNA MARKER)Wirdateti .0Gono SemiadiYulianto .Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, BaliThe aim of this study was to identify the gentic profile of Malay pangolin (Manis javanica) and originpatterns of confiscated specimens.  Tissue samples of Malay pangolin were collected from several confiscatedmaterials in Tangerang, Medan, and Lampung. Wild collections tissue were also conducted in Lampungand Sukabumi. The study was conducted using  conserved Cytochrome b (Cyt. B) DNA mitochondria(mtDNA). The results showed that based on nucleotide base lentgh of 420 nt, confiscated pangolin wasdistributed in three clades and two groups. Haplotype variations was high, consisted of 19 haplotypes in19 individuals (TR1-TR19). On fisrt clade (TR4,7,16,9,19) high substitution occured in adenin base, cladetwo (TR14,17,1,2,15,3,8,13) high substitution occured in guanin base and clade three  (TR5,6,10,11,12) incytosin. It was concluded that haplotipe variation of each populations  was high and for genetic distancebetween individuals was low. Mutation rates was dominated by transition  from guanine to adeninehttp://ojs.unud.ac.id/index.php/jvet/article/view/7682Malay pangolin, Manis javanica, smuggling, cytB mtDNA
spellingShingle Wirdateti .
Gono Semiadi
Yulianto .
Identifikasi Trenggiling (Manis javanica) Menggunakan Penanda Cytochrome B Mitokondria DNA (IDENTIFICATION OF PANGOLIN (MANIS JAVANICA DESMAREST, 1822) USING CYTOCHROME B mtDNA MARKER)
Jurnal Veteriner
Malay pangolin, Manis javanica, smuggling, cytB mtDNA
title Identifikasi Trenggiling (Manis javanica) Menggunakan Penanda Cytochrome B Mitokondria DNA (IDENTIFICATION OF PANGOLIN (MANIS JAVANICA DESMAREST, 1822) USING CYTOCHROME B mtDNA MARKER)
title_full Identifikasi Trenggiling (Manis javanica) Menggunakan Penanda Cytochrome B Mitokondria DNA (IDENTIFICATION OF PANGOLIN (MANIS JAVANICA DESMAREST, 1822) USING CYTOCHROME B mtDNA MARKER)
title_fullStr Identifikasi Trenggiling (Manis javanica) Menggunakan Penanda Cytochrome B Mitokondria DNA (IDENTIFICATION OF PANGOLIN (MANIS JAVANICA DESMAREST, 1822) USING CYTOCHROME B mtDNA MARKER)
title_full_unstemmed Identifikasi Trenggiling (Manis javanica) Menggunakan Penanda Cytochrome B Mitokondria DNA (IDENTIFICATION OF PANGOLIN (MANIS JAVANICA DESMAREST, 1822) USING CYTOCHROME B mtDNA MARKER)
title_short Identifikasi Trenggiling (Manis javanica) Menggunakan Penanda Cytochrome B Mitokondria DNA (IDENTIFICATION OF PANGOLIN (MANIS JAVANICA DESMAREST, 1822) USING CYTOCHROME B mtDNA MARKER)
title_sort identifikasi trenggiling manis javanica menggunakan penanda cytochrome b mitokondria dna identification of pangolin manis javanica desmarest 1822 using cytochrome b mtdna marker
topic Malay pangolin, Manis javanica, smuggling, cytB mtDNA
url http://ojs.unud.ac.id/index.php/jvet/article/view/7682
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AT gonosemiadi identifikasitrenggilingmanisjavanicamenggunakanpenandacytochromebmitokondriadnaidentificationofpangolinmanisjavanicadesmarest1822usingcytochromebmtdnamarker
AT yulianto identifikasitrenggilingmanisjavanicamenggunakanpenandacytochromebmitokondriadnaidentificationofpangolinmanisjavanicadesmarest1822usingcytochromebmtdnamarker