Bacteria Halotolerant from Karst Sinkholes as a Source of Biosurfactants and Bioemulsifiers
Halotolerant bacteria with biosurfactant (BS) and bioemulsifiers (BE) activity can coexist in Karstic sinkholes with marine influence. Two sinkholes in the Yucatan peninsula were selected to isolate bacteria with BE and BS activity stable in NaCl. The optimal time, the effect of nitrogen and carbon...
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MDPI AG
2022-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/7/1264 |
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| author | Félix Maldonado Desena Navila De la Cruz Ceferino Sergio Gómez Cornelio Carina Alvarez Villagomez José Luis Herrera Candelario Susana De la Rosa García |
| author_facet | Félix Maldonado Desena Navila De la Cruz Ceferino Sergio Gómez Cornelio Carina Alvarez Villagomez José Luis Herrera Candelario Susana De la Rosa García |
| author_sort | Félix Maldonado Desena |
| collection | DOAJ |
| description | Halotolerant bacteria with biosurfactant (BS) and bioemulsifiers (BE) activity can coexist in Karstic sinkholes with marine influence. Two sinkholes in the Yucatan peninsula were selected to isolate bacteria with BE and BS activity stable in NaCl. The optimal time, the effect of nitrogen and carbon source in the medium, and the conditions (agitation, pH and salinity) for the production of BS and BE compounds in planktonic and sessile (stimulate the formation of biofilms in cell roller) culture were determined. Eighty strains showed the highest emulsification activity (EI<sub>24</sub> ≥ 50%) and drop-collapse ≥ 4 mm. 87% of the strains are moderately halotolerant, and 21% bordered the limit of extreme halotolerance. Twenty-four strains maintained or improved their BS and BE activity under salinity conditions at 5% and 10%, being the most active genera <i>Bacillus</i>, <i>Paenibacillus</i> and <i>Lysinibacillus</i>, identified by sequencing of the 16S rRNA gene. The results show that the nitrogen source positively affects the BS and BE activity, regardless of the type of culture. The sessile culture markedly stimulated BS activity with significant differences. However, we did not find a greater influence on the culture conditions. The results suggest that halotolerant bacteria from sinkholes could be implemented in bioremediation and other biotechnological applications. |
| first_indexed | 2024-03-09T06:10:51Z |
| format | Article |
| id | doaj.art-c1507c1e27c5462296eb0e180307dc98 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T06:10:51Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c1507c1e27c5462296eb0e180307dc982023-12-03T11:58:27ZengMDPI AGMicroorganisms2076-26072022-06-01107126410.3390/microorganisms10071264Bacteria Halotolerant from Karst Sinkholes as a Source of Biosurfactants and BioemulsifiersFélix Maldonado Desena0Navila De la Cruz Ceferino1Sergio Gómez Cornelio2Carina Alvarez Villagomez3José Luis Herrera Candelario4Susana De la Rosa García5División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carr. Villahermosa-Cardenas Km 0.5, Ranchería Emialiano Zapata, Villahermosa 86150, Tabasco, MexicoDivisión Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carr. Villahermosa-Cardenas Km 0.5, Ranchería Emialiano Zapata, Villahermosa 86150, Tabasco, MexicoIngeniería en Biotecnología, Universidad Politécnica del Centro, Carretera Federal, Villahermosa-Teapa Km 22.5, Tumbulushal Centro, Villahermosa 86290, Tabasco, MexicoDivisión Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carr. Villahermosa-Cardenas Km 0.5, Ranchería Emialiano Zapata, Villahermosa 86150, Tabasco, MexicoDivisión Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carr. Villahermosa-Cardenas Km 0.5, Ranchería Emialiano Zapata, Villahermosa 86150, Tabasco, MexicoDivisión Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carr. Villahermosa-Cardenas Km 0.5, Ranchería Emialiano Zapata, Villahermosa 86150, Tabasco, MexicoHalotolerant bacteria with biosurfactant (BS) and bioemulsifiers (BE) activity can coexist in Karstic sinkholes with marine influence. Two sinkholes in the Yucatan peninsula were selected to isolate bacteria with BE and BS activity stable in NaCl. The optimal time, the effect of nitrogen and carbon source in the medium, and the conditions (agitation, pH and salinity) for the production of BS and BE compounds in planktonic and sessile (stimulate the formation of biofilms in cell roller) culture were determined. Eighty strains showed the highest emulsification activity (EI<sub>24</sub> ≥ 50%) and drop-collapse ≥ 4 mm. 87% of the strains are moderately halotolerant, and 21% bordered the limit of extreme halotolerance. Twenty-four strains maintained or improved their BS and BE activity under salinity conditions at 5% and 10%, being the most active genera <i>Bacillus</i>, <i>Paenibacillus</i> and <i>Lysinibacillus</i>, identified by sequencing of the 16S rRNA gene. The results show that the nitrogen source positively affects the BS and BE activity, regardless of the type of culture. The sessile culture markedly stimulated BS activity with significant differences. However, we did not find a greater influence on the culture conditions. The results suggest that halotolerant bacteria from sinkholes could be implemented in bioremediation and other biotechnological applications.https://www.mdpi.com/2076-2607/10/7/1264biofilmssessile culturesinkholeshalotolerancebioremediationoptimization |
| spellingShingle | Félix Maldonado Desena Navila De la Cruz Ceferino Sergio Gómez Cornelio Carina Alvarez Villagomez José Luis Herrera Candelario Susana De la Rosa García Bacteria Halotolerant from Karst Sinkholes as a Source of Biosurfactants and Bioemulsifiers Microorganisms biofilms sessile culture sinkholes halotolerance bioremediation optimization |
| title | Bacteria Halotolerant from Karst Sinkholes as a Source of Biosurfactants and Bioemulsifiers |
| title_full | Bacteria Halotolerant from Karst Sinkholes as a Source of Biosurfactants and Bioemulsifiers |
| title_fullStr | Bacteria Halotolerant from Karst Sinkholes as a Source of Biosurfactants and Bioemulsifiers |
| title_full_unstemmed | Bacteria Halotolerant from Karst Sinkholes as a Source of Biosurfactants and Bioemulsifiers |
| title_short | Bacteria Halotolerant from Karst Sinkholes as a Source of Biosurfactants and Bioemulsifiers |
| title_sort | bacteria halotolerant from karst sinkholes as a source of biosurfactants and bioemulsifiers |
| topic | biofilms sessile culture sinkholes halotolerance bioremediation optimization |
| url | https://www.mdpi.com/2076-2607/10/7/1264 |
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