Mechanical and biological evaluation of lattice structured hydroxyapatite scaffolds produced via stereolithography additive manufacturing

A photocurable hydroxyapatite (HA) suspensions were prepared and optimized to construct three-dimensional (3D) scaffolds with lattice structures for application in bone tissue engineering. The HA suspensions were categorized into five groups based on the HA contents (designated as HA 30, 32.5, 35, 3...

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Bibliographic Details
Main Authors: Jin-Ho Kang, Kumaresan Sakthiabirami, Kyoung-Jun Jang, Jae-Gon Jang, Gye-Jeong Oh, Chan Park, John G. Fisher, Sang-Won Park
Format: Article
Language:English
Published: Elsevier 2022-02-01
Series:Materials & Design
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0264127521009278
Description
Summary:A photocurable hydroxyapatite (HA) suspensions were prepared and optimized to construct three-dimensional (3D) scaffolds with lattice structures for application in bone tissue engineering. The HA suspensions were categorized into five groups based on the HA contents (designated as HA 30, 32.5, 35, 37.5, and 40 (vol%)). This study demonstrated the applicability of the optimized HA suspension for manufacturing scaffolds using a commercially available stereolithography apparatus (SLA). Disk samples prepared with the HA 35 suspension exhibited the highest bending strength and relative density. Cell attachment experiments revealed that the sintered HA disks did not exhibit cytotoxicity. Furthermore, various types of scaffolds (octahedral, circular, and frame) were designed and constructed using the optimal HA suspension. The designed octahedral scaffold exhibited the highest compressive strength, achieved a break strength improvement of 245% compared with the circular scaffolds, and showed significant differences from the other scaffold types in bone cell proliferation and differentiation experiments. The ceramic suspension formulation proposed herein can be commonly applied for all commercialized 3D SLAs. Furthermore, the use of the octahedral scaffold effectively overcomes the strength issues associated with the ceramic product. This study would aid future research on scaffolds containing various biomaterials and designs prepared using additive manufacturing technology.
ISSN:0264-1275