Mechanical and biological evaluation of lattice structured hydroxyapatite scaffolds produced via stereolithography additive manufacturing
A photocurable hydroxyapatite (HA) suspensions were prepared and optimized to construct three-dimensional (3D) scaffolds with lattice structures for application in bone tissue engineering. The HA suspensions were categorized into five groups based on the HA contents (designated as HA 30, 32.5, 35, 3...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127521009278 |
| _version_ | 1819289155179380736 |
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| author | Jin-Ho Kang Kumaresan Sakthiabirami Kyoung-Jun Jang Jae-Gon Jang Gye-Jeong Oh Chan Park John G. Fisher Sang-Won Park |
| author_facet | Jin-Ho Kang Kumaresan Sakthiabirami Kyoung-Jun Jang Jae-Gon Jang Gye-Jeong Oh Chan Park John G. Fisher Sang-Won Park |
| author_sort | Jin-Ho Kang |
| collection | DOAJ |
| description | A photocurable hydroxyapatite (HA) suspensions were prepared and optimized to construct three-dimensional (3D) scaffolds with lattice structures for application in bone tissue engineering. The HA suspensions were categorized into five groups based on the HA contents (designated as HA 30, 32.5, 35, 37.5, and 40 (vol%)). This study demonstrated the applicability of the optimized HA suspension for manufacturing scaffolds using a commercially available stereolithography apparatus (SLA). Disk samples prepared with the HA 35 suspension exhibited the highest bending strength and relative density. Cell attachment experiments revealed that the sintered HA disks did not exhibit cytotoxicity. Furthermore, various types of scaffolds (octahedral, circular, and frame) were designed and constructed using the optimal HA suspension. The designed octahedral scaffold exhibited the highest compressive strength, achieved a break strength improvement of 245% compared with the circular scaffolds, and showed significant differences from the other scaffold types in bone cell proliferation and differentiation experiments. The ceramic suspension formulation proposed herein can be commonly applied for all commercialized 3D SLAs. Furthermore, the use of the octahedral scaffold effectively overcomes the strength issues associated with the ceramic product. This study would aid future research on scaffolds containing various biomaterials and designs prepared using additive manufacturing technology. |
| first_indexed | 2024-12-24T03:02:21Z |
| format | Article |
| id | doaj.art-c15d445c886d40c89e52ce90a7555858 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-12-24T03:02:21Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-c15d445c886d40c89e52ce90a75558582022-12-21T17:18:10ZengElsevierMaterials & Design0264-12752022-02-01214110372Mechanical and biological evaluation of lattice structured hydroxyapatite scaffolds produced via stereolithography additive manufacturingJin-Ho Kang0Kumaresan Sakthiabirami1Kyoung-Jun Jang2Jae-Gon Jang3Gye-Jeong Oh4Chan Park5John G. Fisher6Sang-Won Park7Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaRIS Foundation for Advanced Biomaterials, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaSchool of Materials Science & Engineering, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of Korea; RIS Foundation for Advanced Biomaterials, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of Korea; Corresponding author at: Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of Korea.A photocurable hydroxyapatite (HA) suspensions were prepared and optimized to construct three-dimensional (3D) scaffolds with lattice structures for application in bone tissue engineering. The HA suspensions were categorized into five groups based on the HA contents (designated as HA 30, 32.5, 35, 37.5, and 40 (vol%)). This study demonstrated the applicability of the optimized HA suspension for manufacturing scaffolds using a commercially available stereolithography apparatus (SLA). Disk samples prepared with the HA 35 suspension exhibited the highest bending strength and relative density. Cell attachment experiments revealed that the sintered HA disks did not exhibit cytotoxicity. Furthermore, various types of scaffolds (octahedral, circular, and frame) were designed and constructed using the optimal HA suspension. The designed octahedral scaffold exhibited the highest compressive strength, achieved a break strength improvement of 245% compared with the circular scaffolds, and showed significant differences from the other scaffold types in bone cell proliferation and differentiation experiments. The ceramic suspension formulation proposed herein can be commonly applied for all commercialized 3D SLAs. Furthermore, the use of the octahedral scaffold effectively overcomes the strength issues associated with the ceramic product. This study would aid future research on scaffolds containing various biomaterials and designs prepared using additive manufacturing technology.http://www.sciencedirect.com/science/article/pii/S0264127521009278Additive manufacturingStereolithography apparatusPhotopolymerization suspensionHydroxyapatiteOctahedral structure |
| spellingShingle | Jin-Ho Kang Kumaresan Sakthiabirami Kyoung-Jun Jang Jae-Gon Jang Gye-Jeong Oh Chan Park John G. Fisher Sang-Won Park Mechanical and biological evaluation of lattice structured hydroxyapatite scaffolds produced via stereolithography additive manufacturing Materials & Design Additive manufacturing Stereolithography apparatus Photopolymerization suspension Hydroxyapatite Octahedral structure |
| title | Mechanical and biological evaluation of lattice structured hydroxyapatite scaffolds produced via stereolithography additive manufacturing |
| title_full | Mechanical and biological evaluation of lattice structured hydroxyapatite scaffolds produced via stereolithography additive manufacturing |
| title_fullStr | Mechanical and biological evaluation of lattice structured hydroxyapatite scaffolds produced via stereolithography additive manufacturing |
| title_full_unstemmed | Mechanical and biological evaluation of lattice structured hydroxyapatite scaffolds produced via stereolithography additive manufacturing |
| title_short | Mechanical and biological evaluation of lattice structured hydroxyapatite scaffolds produced via stereolithography additive manufacturing |
| title_sort | mechanical and biological evaluation of lattice structured hydroxyapatite scaffolds produced via stereolithography additive manufacturing |
| topic | Additive manufacturing Stereolithography apparatus Photopolymerization suspension Hydroxyapatite Octahedral structure |
| url | http://www.sciencedirect.com/science/article/pii/S0264127521009278 |
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