Transposase-assisted tagmentation of RNA/DNA hybrid duplexes

Transposase-assisted tagmentation of RNA/DNA hybrid duplexes

Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-throughput sequencing applications. Here, we report that the Tn5 transposase is also capable of direct tagmentation of RNA/DNA hybrids in vitro. As a proof-of-concept application, we utilized this activity to re...

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Bibliographic Details
Main Authors: Bo Lu, Liting Dong, Danyang Yi, Meiling Zhang, Chenxu Zhu, Xiaoyu Li, Chengqi Yi
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2020-07-01
Series:eLife
Subjects:
RNA/DNA hybrids
Tn5
tagmentation
transposition
RNA-seq
epitranscriptome
Online Access:https://elifesciences.org/articles/54919

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